IFT80 (NM_001190242) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ATD2; FAP167; SRTD2; WDR56
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329415 representing NM_001190242. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCTTAGATCAACTTTAGCTCAGCAAGGAACACCAGTGTATTCAGTAGCGTGGGGCCCTGATTCAGAA AAGGTTCTTTATACAGCAGGCAAGCAGCTAATCATTAAACCTCTTCAACCAAATGCTAAAGTTTTGCAG TGGAAAGCTCATGATGGCATTATTTTAAAAGTAGATTGGAACTCGGTCAATGATCTTATTTTATCTGCT GGTGAAGACTGTAAATATAAGGTATGGGATAGTTACGGCCGCCCACTGTACAATTCACAACCTCATGAG CATCCCATTACTTCAGTTGCCTGGGCTCCAGATGGAGAATTATTTGCTGTTGGATCGTTTCATACTTTA CGCTTGTGTGATAAAACTGGGTGGTCATATGCATTAGAAAAACCCAACACTGGCAGCATATTTAATATT GCATGGTCTATCGATGGCACTCAGATTGCTGGAGCCTGTGGAAATGGACATGTCGTTTTTGCACATGTG GTGGAACAACATTGGGAGTGGAAAAATTTTCAAGTAACATTAACGAAAAGAAGAGCCATGCAGGTTCGT AATGTTCTTAATGATGCAGTGGATTTACTGGAATTCCGTGATAGAGTCATTAAAGCATCTTTGAACTAT GCACACTTAGTTGTTTCAACGTCTCTTCAATGTTACGTGTTCTCCACGAAGAACTGGAACACACCAATT ATATTTGATCTCAAAGAAGGAACTGTTAGTTTGATTCTGCAGGCAGAAAGACATTTTCTTCTTGTAGAT GGTAGTAGTATCTATTTATATTCATATGAAGGGCGCTTTATTTCATCTCCAAAATTTCCTGGAATGAGA ACAGATATTCTGAATGCACAGACTGTGTCTTTGAGTAATGATACCATAGCAATAAGAGACAAAGCTGAT GAAAAAATAATCTTCCTCTTTGAGGCATCAACCGGAAAGCCGTTAGGTGATGGAAAGTTTCTTTCTCAT AAGAATGAAATCTTGGAAATTGCTCTGGATCAAAAAGGACTTACCAATGATAGAAAAATTGCTTTCATT GATAAAAATAGAGATCTCTGTATCACTTCTGTGAAACGATTTGGGAAGGAAGAACAAATTATCAAGCTT GGAACAATGGTGCATACTTTGGCATGGAACGATACATGCAATATCCTTTGTGGACTTCAAGATACTCGA TTTATAGTGTGGTATTACCCCAATACAGTTTATGTGGACAGAGACATTTTGCCTAAAACATTATATGAA AGGGATGCAAGTGAATTTAGTAAAAATCCCCATATTGTGAGTTTTGTTGGAAATCAAGTAACTATTAGA AGAGCTGATGGCTCCCTGGTTCACATCAGCATAACACCATATCCTGCTATTCTCCATGAATATGTAAGC AGTTCAAAATGGGAAGATGCTGTGAGACTTTGTCGCTTTGTTAAGGAGCAAACCATGTGGGCTTGTCTA GCTGCTATGGCAGTTGCTAATCGAGATATGACTACTGCAGAAATAGCCTATGCAGCAATTGGTGAAATT GATAAGGTTCAGTACATCAATTCTATAAAAAATCTTCCATCTAAAGAATCAAAAATGGCCCACATACTA CTGTTTAGTGGGAACATACAGGAGGCTGAAATAGTACTTCTTCAGGCTGGCCTTGTTTATCAAGCAATC CAGATCAATATTAATCTCTACAACTGGGAAAGGGCACTGGAATTGGCTGTAAAATACAAAACACATGTT GATACAGTTCTTGCTTACCGTCAAAAGTTTTTGGAGACATTTGGTAAACAGGAAACTAATAAACGATAC TTGCATTATGCAGAAGGTCTCCAAATAGATTGGGAGAAAATCAAAGCCAAAATTGAGATGGAAATTACA AAAGAAAGAGAGCAATCATCAAGCAGCCAATCCAGCAAGAGTATAGGTTTAAAGCCCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001190242
Insert Size	1923 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001190242.1
RefSeq Size	4176 bp
RefSeq ORF	1923 bp
Locus ID	57560
UniProt ID	Q9P2H3
MW	72.6 kDa
Gene Summary	The protein encoded by this gene is part of the intraflagellar transport complex B and is necessary for the function of motile and sensory cilia. Defects in this gene are a cause of asphyxiating thoracic dystrophy 2 (ATD2). Three transcript variants encoding two different isoforms have been found for this gene.[provided by RefSeq, Jun 2010] Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2 and 3 both encode the same isoform.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.