RGNEF (ARHGEF28) (NM_001177693) Human Untagged Clone
CAT＃: SC329369
ARHGEF28 (untagged)-Human 190 kDa guanine nucleotide exchange factor (RGNEF) transcript variant 2
CNY 26790.00
货期*
9周
规格
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | p190RHOGEF; RGNEF; RIP2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001177693, the custom clone sequence may differ by one or more nucleotides
ATGGAGTTGAGCTGCAGCGAAGCACCTCTTTACGGGCAGATGATGATCTATGCGAAGTTT GACAAAAATGTGTATCTTCCTGAAGATGCTGAGTTTTACTTTACTTATGACGGATCTCAT CAGCGACATGTCATGATTGCAGAGCGCATCGAGGATAACGTTCTCCAGTCCAGCGTCCCA GGCCATGGGCTTCAGGAGACGGTGACGGTATCTGTGTGCCTCTGCTCGGAAGGTTACTCT CCGGTGACCATGGGCTCTGGCTCAGTGACCTACGTGGACAACATGGCTTGCAGGCTGGCT CGTCTGCTGGTGACGCAGGCCAATCGCCTCACAGCCTGCAGCCACCAGACCCTGCTGACC CCATTTGCCTTGACGGCAGGAGCACTGCCTGCCTTGGATGAGGAGCTCGTGCTGGCTCTG ACCCATCTGGAATTGCCTCTAGAGTGGACTGTGTTGGGAAGTTCTTCACTTGAAGTATCT TCTCACAGAGAATCTCTTCTACACCTGGCTATGAGATGGGGCCTGGCTAAACTTTCCCAG TTCTTCTTGTGTCTCCCGGGGGGAGTCCAGGCCTTGGCTTTACCCAACGAAGAGGGTGCC ACACCATTAGACTTAGCTTTACGTGAAGGACACTCCAAGCTGGTGGAAGACGTCACAAAT TTTCAGGGCAGATGGTCCCCAAGCTTCTCCCGAGTGCAGCTCAGTGAAGAAGCCTCCTTG CATTACATTCACTCATCGGAAACGCTGACCCTGACCCTGAACCACACAGCCGAGCATTTG TTGGAGGCAGATATTAAACTCTTCCGGAAATACTTTTGGGATAGAGCCTTTCTTGTCAAG GCCTTTGAGCCAGAAGCCAGGCCAGAGGAAAGAACAGCTATGCCCTCCAGCGGTGCAGAA ACTGAAGAAGAGATTAAGAATTCAGTGTCCAGCAGATCAGCAGCTGAAAAGGAAGATATA AAGCGTGTCAAAAGCCTGGTGGTTCAACACAATGAACATGAAGACCAGCACAGCCTAGAT TTGGATCGCTCCTTCGATATCCTAAAAAAATCCAAGCCGCCCTCGACATTGCTTGCTGCA GGCCGGCTTTCAGACATGCTGAATGGAGGTGATGAAGTCTACGCTAACTGTATGGTGATT GATCAGGTTGGTGATTTGGATATCAGCTATATTAATATAGAGGGAATCACTGCCACTACC AGCCCTGAATCCAGAGGTTGCACTCTGTGGCCTCAGAGCAGCAAACACACCCTTCCTACA GAAACCAGTCCCAGTGTGTACCCACTTAGTGAAAATGTCGAAGGGACAGCACACACTGAA GCCCAGCAGTCCTTCATGTCACCATCAAGTTCGTGTGCTTCCAACTTGAATCTTTCTTTT GGTTGGCATGGATTTGAAAAGGAACAAAGTCATCTAAAGAAAAGAAGTTCTAGCCTTGAT GCCTTGGACGCCGACAGTGAAGGGGAAGGGCATTCTGAGCCATCCCACATCTGTTACACT CCAGGGTCTCAGAGCTCCTCAAGAACTGGGATTCCTAGTGGGGATGAATTGGACTCTTTT GAGACTAACACTGAACCGGATTTTAATATCTCCAGGGCTGAATCCCTTCCTCTATCAAGT AATCTACAGTCGAAGGAATCACTGCTTTCTGGAGTTCGCTCACGTTCTTATTCTTGCTCA TCACCCAAAATTTCTTTAGGAAAAACTCGTTTGGTGCGTGAATTAACAGTATGCAGTTCA AGTGAAGAGCAAAGAGCTTACAGCTTATCGGAGCCACCAAGAGAAAACAGAATTCAGGAA GAAGAATGGGATAAATACATCATACCTGCCAAATCAGAGTCTGAAAAATATAAAGTGAGT CGAACTTTCAGTTTCCTCATGAATAGGATGACTAGCCCTCGGAATAAATCAAAGACAAAA AGCAAGGATGCCAAAGATAAAGAGAAGCTGAATCGACATCAGTTTGCCCCAGGAACATTC TCTGGGGTTCTGCAGTGTTTGGTTTGTGATAAAACACTCCTGGGGAAAGAGTCACTGCAG TGTTCTAACTGTAATGCAAATGTGCACAAAGGTTGTAAAGATGCTGCGCCTGCATGCACC AAGAAATTCCAAGAGAAATATAACAAGAACAAACCACAGACCATCCTTGGAAATTCTTCA TTTAGAGACATCCCACAGCCTGGTCTCTCCTTGCACCCTTCTTCCTCCGTGCCTGTTGGA TTGCCGACTGGAAGGAGGGAGACTGTGGGACAGGTCCATCCATTGTCCAGAAGTGTTCCA GGCACCACCTTGGAAAGCTTCAGGAGGTCAGCCACATCCTTGGAGTCTGAGAGTGACCAT AACAGCTGCAGAAGCAGGTCTCATTCTGATGAGCTGCTACAGTCCATGGGCTCTTCTCCC TCTACAGAGTCTTTCATAATGGAAGATGTTGTGGATTCTTCTCTGTGGAGTGACCTCAGC AGTGATGCCCAGGAGTTTGAAGCAGAATCTTGGAGTCTTGTGGTGGATCCCTCATTTTGT AATAGGCAGGAGAAGGATGTCATCAAAAGACAGGATGTCATTTTTGAGCTAATGCAAACA GAGATGCATCACATCCAGACCCTGTTCATCATGTCTGAGATCTTCAGGAAAGGCATGAAA GAGGAGCTGCAGCTGGACCACAGCACCGTGGATAAAATTTTCCCCTGTTTAGATGAGTTG CTTGAAATCCACAGGCATTTCTTCTACAGTATGAAGGAACGAAGGCAGGAATCCTGTGCT GGCAGCGACAGGAATTTTGTGATCGACCGAATTGGAGATATTTTGGTACAACAGTTTTCA GAAGAAAATGCAAGTAAAATGAAGAAAATATATGGAGAATTCTGTTGCCATCATAAAGAA GCTGTTAACCTCTTTAAAGAACTCCAGCAGAATAAAAAGTTTCAGAATTTTATTAAGCTC CGAAATAGTAATCTTTTGGCTCGACGCCGAGGAATTCCAGAATGCATTCTGTTGGTCACT CAGCGTATTACAAAATACCCTGTCTTGGTGGAAAGGATATTGCAGTACACAAAGGAAAGA ACTGAGGAACATAAAGACTTACGCAAAGCGCTTTGCTTAATTAAAGACATGATTGCAACA GTGGATTTAAAAGTCAATGAATATGAGAAAAACCAAAAATGGCTTGAGATCCTAAATAAG ATTGAAAACAAAACATACACGAAGCTCAAAAATGGACATGTGTTTAGGAAGCAGGCACTG ATGAGTGAAGAAAGGACTCTGTTATATGATGGCCTTGTTTACTGGAAAACTGCTACAGGT CGTTTCAAAGATATCCTAGCTCTACTTCTAACTGATGTGCTGCTCTTTTTACAAGAAAAA GACCAGAAATACATCTTTGCAGCCGTTGATCAGAAGCCATCAGTTATTTCCCTTCAAAAG CTTATTGCTAGAGAAGTTGCTAATGAGGAGAGAGGAATGTTTCTGATCAGTGCTTCATCT GCTGGTCCTGAGATGTATGAAATTCACACCAATTCCAAGGAGGAACGCAATAACTGGATG AGACGGATCCAGCAGGCTGTAGAAAGTTGTCCTGAAGAAAAAGGGGGAAGGACAAGTGAA TCTGATGAAGACAAGAGGAAAGCTGAAGCCAGAGTGGCCAAAATTCAGCAATGTCAAGAA ATACTCACTAACCAAGACCAACAAATTTGTGCGTATTTGGAGGAGAAGCTGCATATCTAT GCTGAACTTGGAGAACTGAGCGGATTTGAGGACGTCCATCTAGAGCCCCACCTCCTTATT AAACCTGACCCAGGCGAGCCTCCCCAGGCAGCCTCATTACTGGCAGCAGCACTGAAAGAA GCTGAGAGCCTACAAGTTGCAGTGAAGGCCTCACAGATGGGCGCCGTGAGTCAATCATGT GAGGACAGTTGTGGAGACTCTGTCTTGGCGGACACACTCAGTTCTCATGATGTACCAGGA TCACCGACTGCCTCATTAGTCACAGGAGGGAGAGAAGGAAGAGGCTGTTCGGATGTGGAT CCCGGGATCCAGGGTGTGGTAACCGACTTGGCCGTCTCTGATGCAGGGGAGAAGGTGGAA TGTAGAAATTTTCCAGGTTCTTCACAATCAGAGATTATACAAGCCATACAGAATTTAACC CGTCTCTTATACAGCCTTCAGGCCGCCTTGACCATTCAGGACAGCCACATTGAGATCCAC AGGCTGGTTCTCCAGCAGCAGGAGGGCCTGTCTCTCGGCCACTCTATCCTCCGAGGCGGC CCCTTGCAGGACCAGAAGTCTCGCGACGCGGACAGGCAGCATGAGGAGCTGGCCAATGTG CACCAGCTTCAGCACCAGCTCCAGCAGGAGCAGCGGCGCTGGCTGCGCAGGTGTGAGCAG CAGCAGCGGGCGCAGGCGACCAGGGAGAGCTGGCTGCAGGAGCGGGAGCGGGAGTGCCAG TCGCAGGAGGAGCTGCTGCTGCGGAGCCGGGGCGAGCTGGACCTCCAGCTCCAGGAGTAC CAGCACAGCCTGGAGCGGCTGAGGGAGGGCCAGCGCCTGGTGGAGAGGGAGCAGGCGAGG ATGCGGGCCCAGCAGAGCCTGCTGGGCCACTGGAAGCACGGCCGGCAGAGGAGCCTGCCC GCGGTGCTCCTTCCGGGTGGCCCCGAGGTAATGGAACTTAATCGATCTGAGAGTTTATGT CATGAAAACTCATTCTTCATCAATGAAGCTTTAGTACAAATGTCATTTAACACTTTCAAC AAACTGAATCCATCAGTTATCCATCAGGATGCCACTTACCCTACAACTCAATCTCATTCT GACTTGGTGAGGACTAGTGAACATCAAGTAGACCTCAAGGTGGACCCTTCTCAGCCTTCG AATGTCAGTCACAAACTGTGGACAGCCGCTGGTTCCGGCCATCAGATACTTCCTTTCCAT GAAAGCAGCAAGGATTCTTGTAAAAATGATTTGGACACCTCCCACACTGAGTCCCCAACC CCCCATGACTCAAATTCACACCGCCCTCAACTGCAGGCGTTTATAACAGAAGCAAAGCTA AATCTACCGACAAGGACAATGACCAGACAAGATGGGGAAACTGGAGATGGAGCCAAAGAA AATATTGTTTACCTCTAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001177693 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001177693.1, NP_001171164.1 |
| RefSeq Size | 6291 bp |
| RefSeq ORF | 5118 bp |
| Locus ID | 64283 |
| UniProt ID | Q8N1W1 |
| Gene Summary | This gene encodes a member of the Rho guanine nucleotide exchange factor family. The encoded protein interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. |
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