OBSL1 (NM_001173431) Human Untagged Clone
CAT＃: SC329362
OBSL1 (untagged)-Human obscurin-like 1 (OBSL1) transcript variant 2
CNY 29640.00
货期*
详询
规格
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001173431, the custom clone sequence may differ by one or more nucleotides
ATGAAGGCGAGCTCGGGGGATCAGGGGAGCCCCCCGTGCTTCCTGCGCTTCCCGCGGCCT GTGCGGGTGGTAAGTGGCGCCGAGGCCGAGCTCAAGTGCGTGGTCCTGGGGGAGCCGCCG CCTGTAGTGGTGTGGGAGAAGGGCGGGCAGCAGCTGGCGGCCTCGGAACGCCTGAGCTTC CCGGCGGACGGCGCGGAGCACGGCCTGCTGCTGACCGCCGCACTGCCCACCGACGCGGGG GTCTACGTGTGCCGCGCCCGCAACGCGGCCGGCGAGGCCTACGCGGCGGCCGCCGTCACC GTGCTGGAGCCGCCGGCCTCCGACCCCGAGCTGCAGCCCGCCGAGCGCCCGCTGCCATCG CCGGGGTCCGGGGAGGGCGCCCCGGTCTTCCTCACGGGGCCTCGATCCCAGTGGGTGCTG CGGGGGGCGGAGGTGGTGCTGACGTGCCGGGCGGGGGGCCTCCCCGAGCCCACACTGTAC TGGGAGAAGGACGGGATGGCCCTGGACGAAGTGTGGGACAGCAGCCACTTCGCGCTCCAG CCGGGCCGCGCCGAGGACGGCCCCGGCGCGAGCCTGGCACTGCGCATCCTGGCGGCTCGG CTGCCGGATTCCGGCGTCTACGTGTGCCACGCCCGCAACGCGCACGGCCACGCGCAGGCG GGGGCGCTGCTCCAGGTGCACCAGCCCCCCGAGAGCCCGCCCGCGGACCCCGACGAGGCC CCCGCGCCGGTGGTGGAGCCGCTCAAGTGCGCGCCTAAGACCTTCTGGGTGAACGAGGGC AAGCACGCCAAGTTCCGCTGCTACGTGATGGGCAAGCCCGAGCCCGAGATCGAATGGCAC TGGGAGGGCCGCCCGCTGCTCCCGGACCGCCGCCGCCTCATGTACCGCGACCGCGACGGC GGCTTCGTGCTCAAGGTGCTTTACTGCCAGGCCAAGGATCGTGGGCTCTACGTCTGCGCC GCGCGCAACTCGGCGGGCCAGACGCTCAGTGCCGTGCAGCTGCACGTGAAAGAGCCCCGC CTCCGGTTCACACGGCCCCTGCAGGACGTGGAGGGCCGTGAGCACGGGATTGCCGTGCTG GAATGTAAAGTACCCAACTCCCGCATCCCCACGGCCTGGTTCCGTGAGGACCAGCGGCTG CTGCCCTGCCGCAAGTACGAGCAGATCGAAGAGGGCACTGTCCGGCGCCTCATCATCCAC AGGCTGAAGGCAGACGATGATGGTATCTACCTGTGCGAGATGCGGGGCCGGGTGCGCACC GTGGCCAACGTCACAGTCAAAGGGCCCATCCTGAAGCGCCTGCCCCGGAAGCTCGACGTC CTGGAAGGAGAGAATGCTGTGCTGCTAGTGGAAACTCTAGAGGCCGGGGTCGAGGGACGC TGGAGCCGTGATGGGGAGGAGCTGCCGGTCATCTGCCAGAGCAGCTCAGGCCACATGCAT GCCCTGGTCCTTCCAGGGGTCACCCGAGAGGATGCTGGCGAGGTCACCTTTAGCCTGGGC AACTCCCGTACCACTACGCTTCTCAGAGTAAAATGTGTCAAGCACAGTCCCCCAGGACCC CCCATATTGGCAGAGATGTTCAAGGGCCACAAGAACACGGTCCTGTTGACCTGGAAGCCT CCCGAGCCAGCTCCCGAGACCCCATTCATCTACCGGCTGGAGCGGCAGGAAGTGGGCTCT GAAGACTGGATTCAGTGCTTCAGCATCGAGAAAGCCGGAGCCGTGGAGGTGCCGGGCGAC TGTGTGCCCTCCGAGGGTGACTACCGCTTCCGCATCTGCACAGTCAGCGGACATGGCCGT AGTCCCCACGTGGTGTTCCACGGTTCTGCTCACCTTGTGCCCACAGCTCGCCTGGTGGCA GGTCTGGAGGATGTGCAGGTATACGACGGGGAAGATGCCGTCTTCTCCCTCGATCTCTCC ACCATCATCCAGGGTACCTGGTTCCTTAATGGGGAAGAGCTCAAGAGTAACGAGCCGGAG GGCCAGGTGGAACCTGGGGCCCTGCGGTACCGTATAGAGCAGAAGGGTCTGCAGCACAGA CTCATCCTGCATGCCGTCAAGCACCAGGACAGCGGTGCCCTGGTCGGCTTCAGCTGCCCC GGCGTGCAGGACTCAGCTGCCCTCACAATCCAAGAGAGCCCGGTGCACATCCTGAGCCCC CAGGACAGGGTGTCGTTGACCTTCACAACCTCAGAGCGGGTGGTGCTGACTTGTGAGCTC TCAAGGGTGGACTTCCCGGCAACCTGGTACAAGGATGGGCAGAAGGTGGAGGAGAGCGAG TTGCTGGTGGTGAAGATGGATGGGCGCAAACACCGTCTGATCCTGCCTGAGGCCAAAGTC CAGGACAGTGGCGAGTTTGAGTGCAGGACAGAAGGGGTCTCGGCCTTCTTCGGCGTCACT GTCCAAGATCCTCCCGTGCACATCGTGGACCCCCGAGAACATGTGTTCGTGCATGCCATA ACTTCCGAGTGTGTCATGCTGGCCTGTGAGGTGGACCGAGAGGACGCCCCTGTGCGTTGG TACAAGGACGGGCAGGAGGTGGAGGAGAGTGACTTCGTGGTGCTGGAGAATGAGGGGCCC CATCGCCGCCTGGTGCTGCCCGCCACCCAGCCCTCAGACGGGGGCGAGTTTCAGTGCGTC GCTGGAGATGAGTGTGCCTACTTCACTGTCACCATCACAGACGTCTCCTCGTGGATCGTG TATCCCAGCGGCAAGGTGTATGTGGCAGCCGTGCGCCTGGAGCGTGTGGTGCTGACCTGT GAGCTATGCCGGCCCTGGGCAGAGGTGCGCTGGACCAAGGATGGAGAGGAGGTGGTGGAG AGCCCCGCGCTGCTCCTGCAGAAGGAAGACACTGTCCGCCGCCTGGTGCTGCCCGCTGTC CAGCTCGAGGACTCCGGCGAGTACTTGTGTGAAATTGACGATGAGTCGGCCTCCTTCACT GTCACCGTCACAGAACCCCCAGTGCGGATCATATACCCTCGCGATGAGGTGACCTTGATC GCCGTGACCTTGGAGTGTGTGGTGCTGATGTGTGAACTGTCTCGGGAGGATGCCCCTGTG CGCTGGTACAAGGATGGGCTGGAAGTGGAGGAGAGCGAGGCCCTGGTGCTGGAGAGGGAT GGGCCACGCTGCCGCCTGGTGCTACCTGCTGCTCAGCCCGAGGACGGGGGCGAGTTTGTA TGTGATGCTGGAGATGACTCGGCCTTCTTCACTGTCACTGTCACAGCCCCACCAGAGAGG ATTGTGCACCCGGCAGCCCGCTCCCTGGATCTGCATTTTGGGGCTCCAGGGCGCGTGGAG CTGCGCTGTGAGGTGGCCCCAGCTGGGTCTCAGGTGCGCTGGTACAAGGACGGGCTGGAA GTGGAGGCATCAGATGCCCTGCAGCTGGGTGCCGAGGGGCCCACCCGCACCCTGACCCTG CCCCACGCCCAGCCTGAGGACGCCGGGGAGTATGTGTGTGAGACCCGGCATGAGGCCATC ACCTTCAATGTCATCCTGGCTGAGCCTCCAGTGCAGTTCCTTGCTCTAGAGACAACTCCA AGCCCGCTCTGTGTGGCCCCTGGGGAGCCAGTGGTGCTGAGCTGTGAACTGTCCCGGGCT GGCGCCCCCGTGGTCTGGAGCCACAATGGGAGGCCCGTGCAGGAGGGCGAGGGCCTAGAG CTCCATGCCGAGGGCCCCCGCCGAGTCCTCTGCATCCAGGCTGCAGGCCCAGCCCATGCA GGGCTCTACACCTGCCAGTCTGGAGCAGCCCCCGGAGCCCCAAGCCTCAGCTTCACCGTC CAGGTGGCTGAGCCCCCTGTGCGGGTGGTAGCTCCCGAGGCAGCCCAGACGAGGGTTCGG AGCACCCCAGGCGGGGACCTAGAGCTGGTGGTGCACCTCTCCGGGCCAGGGGGCCCTGTA CGCTGGTACAAGGACGGGGAGCGACTGGCAAGCCAGGGGCGGGTGCAGCTGGAGCAGGCC GGGGCCAGGCAGGTGCTGCGGGTGCAGGGGGCACGGAGCGGGGACGCTGGGGAGTACCTG TGCGATGCGCCCCAGGACAGCCGCATCTTCCTTGTCAGCGTGGAAGAGCCACTGCTGGTG AAGCTGGTCTCGGAGCTGACACCACTCACTGTCCACGAGGGCGATGATGCCACGTTCCGG TGTGAAGTCTCCCCACCAGATGCCGATGTCACCTGGCTGCGCAATGGGGCCGTCGTCACT CCAGGGCCCCAGGTGGAGATGGCCCAGAATGGTTCAAGCCGCATCTTAACCTTGCGAGGC TGCCAACTGGGGGATGCAGGGACCGTGACTTTGCGGGCAGGGAGCACGGCCACAAGTGCC CGGCTCCATGTTCGAGAGACAGAGCTGCTGTTCCTACGGCGGTTGCAGGATGTGCGGGCA GAGGAAGGCCAGGATGTGTGTCTCGAAGTGGAGACAGGCCGAGTGGGTGCAGCGGGGGCC GTGCGCTGGGTGCGAGGTGGGCAGCCCCTGCCCCACGACTCTCGCCTGTCCATGGCCCAG GATGGGCACATCCACCGCCTCTTCATCCATGGTGTCATACTGGCCGACCAGGGCACCTAC GGCTGCGAGAGCCACCACGATCGCACCCTGGCCAGGCTCAGCGTGAGGCGTGAGTGCCCC GTCCTCTCTTAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001173431 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001173431.1, NP_001166902.1 |
| RefSeq Size | 5793 bp |
| RefSeq ORF | 4632 bp |
| Locus ID | 23363 |
| UniProt ID | O75147 |
| Gene Summary | Cytoskeletal adaptor proteins function in linking the internal cytoskeleton of cells to the cell membrane. This gene encodes a cytoskeletal adaptor protein, which is a member of the Unc-89/obscurin family. The protein contains multiple N- and C-terminal immunoglobulin (Ig)-like domains and a central fibronectin type 3 domain. Mutations in this gene cause 3M syndrome type 2. Alternatively spliced transcript variants encoding different isoforms have been found in this gene. [provided by RefSeq, Mar 2010] Transcript Variant: This variant (2) lacks multiple 3' exons but has an alternate 3' sequence, as compared to variant 1. The resulting isoform (2, also known as isoform C) is shorter and has a different C-terminus, as compared to isoform 1. |
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