LAS1L (NM_001170650) Human Untagged Clone
CAT＃: SC329056
LAS1L (untagged)-Human LAS1-like (S. cerevisiae) (LAS1L) transcript variant 3
CNY 13110.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | dJ475B7.2; Las1; Las1-like; WTS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC329056 representing NM_001170650.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGTGGGAATCCGGGGCCGGGCCAGGTCTAGGTTCCCAGGGGATGGATCTCGTGTGGAGTGCGTGG TACGGAAAGTGCGTTAAAGGGAAAGGGTCGTTGCCACTCTCGGCCCACGGCATCGTGGTCGCCTGGCTC AGCAGGGCCGAGTGGGACCAGGTGACGGTTTATCTGTTCTGTGACGACCATAAGTTGCAGCGGTACGCG CTTAACCGCATCACGGTGTGGAGGAGCAGGTTTGTGAATCTTATCTCAGAGAGGAAGACAAAGTTTGCC AAGGTCCCCCTCAAGTGTCTGGCTCAAGAGGTAAATATTCCGGATTGGATTGTTGACCTTCGCCATGAG TTGACCCACAAGAAAATGCCCCATATAAATGACTGCCGCAGAGGCTGCTACTTTGTCCTGGATTGGCTC CAGAAGACCTATTGGTGCCGCCAACTGGAGAACAGCCTGAGAGAGACCTGGGAGTTGGAGGAGTTCAGG GAAGGGATAGAGGAAGAGGATCAAGAGGAAGATAAGAACATTGTTGTTGATGACATCACAGAACAGAAA CCAGAGCCTCAGGATGATGGGAAAAGTACGGAGTCAGATGTAAAGGCCGATGGAGACAGCAAAGGCAGC GAAGAGGTGGATTCTCATTGCAAAAAGGCCCTGAGTCATAAAGAGCTATATGAAAGAGCCCGAGAACTG CTGGTATCATACGAAGAGGAGCAGTTTACGGTGCTGGAGAAATTTAGGTATTTACCTAAGGCCATTAAG GCGTGGAATAACCCGTCCCCACGTGTAGAATGTGTCCTGGCAGAGCTCAAGGGCGTTACATGCGAGAAC AGGGAGGCTGTGCTGGATGCTTTTCTGGATGATGGCTTCCTTGTCCCCACATTTGAACAGTTGGCAGCT TTGCAGATAGAATATGAAGAAAACGTGGACTTGAATGACGTCCTGGTGCCAAAGCCGTTCTCTCAGTTC TGGCAGCCCCTGCTCAGGGGCCTGCACTCCCAGAACTTCACGCAGGCCCTATTGGAGAGGATGCTCTCT GAACTGCCAGCCTTGGGGATCAGCGGGATCCGGCCTACCTACATCCTCAGATGGACCGTTGAACTGATC GTGGCCAACACCAAGACTGGACGGAATGCTCGCCGATTTTCTGCAGGCCAGTGGGAAGCAAGAAGGGGC TGGAGGCTGTTCAACTGCTCCGCCTCCCTTGACTGGCCCCGGATGGTTGAGTCCTGCTTGGGCTCACCT TGCTGGGCCAGCCCCCAACTCCTTCGGATCATCTTCAAAGCCATGGGGCAGGGCCTGCCAGACGAGGAG CAGGAGAAGCTGCTGCGCATCTGTTCCATTTATACCCAGAGTGGAGAAAACAGCCTGGTGCAGGAGGGC TCTGAGGCCTCCCCCATTGGGAAGTCTCCATATACACTAGACAGCCTGTATTGGAGCGTCAAGCCAGCC AGCTCCAGCTTCGGGTCTGAAGCAAAGGCCCAGCAACAGGAGGAGCAGGGCAGTGTTAATGATGTCAAG GAAGAGGAGAAGGAGGAGAAAGAGGTCTTGCCAGACCAGGTAGAGGAGGAGGAAGAAAATGATGACCAA GAGGAGGAAGAGGAGGATGAAGATGATGAAGATGATGAAGAGGAAGACAGAATGGAGGTGGGGCCTTTC TCTACAGGGCAAGAGTCCCCCACTGCCGAGAATGCTAGGCTTCTGGCCCAGAAAAGAGGAGCTTTGCAG GGCTCTGCATGGCAGGTTAGCTCAGAAGACGTGCGATGGGACACATTTCCCCTAGGCCGAATGCCAGGT CAGACCGAGGACCCAGCAGAGCTCATGCTGGAGAATTATGACACCATGTATCTTTTGGACCAGCCTGTG CTAGAGCAGCGGCTGGAACCCTCAACATGCAAGACTGACACCTTGGGCCTGAGCTGTGGTGTCGGCAGT GGCAACTGCAGCAACAGCAGCAGCAGCAACTTCGAGGGCCTTCTCTGGAGCCAGGGGCAGCTGCATGGG CTCAAAACTGGCCTGCAGCTCTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001170650 |
| Insert Size | 2028 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001170650.1 |
| RefSeq Size | 2322 bp |
| RefSeq ORF | 2028 bp |
| Locus ID | 81887 |
| UniProt ID | Q9Y4W2 |
| MW | 76.8 kDa |
| Gene Summary | Involved in the biogenesis of the 60S ribosomal subunit. Required for maturation of the 28S rRNA. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks two alternate in-frame exons in the coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230418 | LAS1L (Myc-DDK-tagged)-Human LAS1-like (S. cerevisiae) (LAS1L), transcript variant 3 |
CNY 4944.00 |
|
| RC230418L3 | Lenti ORF clone of Human LAS1-like (S. cerevisiae) (LAS1L), transcript variant 3, Myc-DDK-tagged |
CNY 9120.00 |
|
| RC230418L4 | Lenti ORF clone of Human LAS1-like (S. cerevisiae) (LAS1L), transcript variant 3, mGFP tagged |
CNY 9120.00 |
|
| RG230418 | LAS1L (tGFP-tagged) - Human LAS1-like (S. cerevisiae) (LAS1L), transcript variant 3 |
CNY 7980.00 |
