Kaiso (ZBTB33) (NM_001184742) Human Untagged Clone
CAT＃: SC329054
ZBTB33 (untagged)-Human zinc finger and BTB domain containing 33 (ZBTB33) transcript variant 1
CNY 13020.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ZNF-kaiso; ZNF348 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC329054 representing NM_001184742.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGAGTAGAAAACTGATTTCTGCTACAGACATTCAGTACTCTGGCAGTCTGCTGAACTCCTTGAAT GAGCAACGTGGCCATGGACTCTTCTGTGATGTTACCGTTATTGTGGAAGACCGAAAATTCCGGGCTCAC AAGAATATTCTTTCAGCTTCTAGTACCTACTTCCATCAGCTCTTCTCTGTTGCTGGGCAAGTTGTTGAA CTGAGCTTTATAAGAGCAGAGATCTTTGCAGAAATTCTCAATTATATCTATAGTTCTAAAATTGTTCGT GTTAGATCAGATTTGCTTGATGAGTTAATTAAATCAGGGCAGTTATTAGGAGTGAAATTTATAGCAGAG CTTGGTGTCCCATTGTCACAGGTTAAAAGCATCTCAGGTACAGCGCAGGATGGTAATACTGAGCCTTTA CCTCCTGATTCTGGTGACAAGAACCTTGTAATACAGAAATCAAAAGATGAAGCCCAAGATAATGGGGCT ACTATAATGCCTATTATAACAGAGTCTTTTTCATTATCTGCCGAAGATTATGAAATGAAAAAGATCATT GTTACCGATTCTGATGATGATGATGATGATGTCATTTTTTGCTCCGAGATTCTGCCCACAAAGGAGACT TTGCCGAGTAATAACACAGTGGCACAGGTCCAATCTAACCCAGGCCCTGTTGCTATTTCAGATGTTGCA CCTAGTGCTAGCAATAACTCGCCCCCTTTAACAAATATCACACCTACTCAGAAACTTCCTACTCCTGTG AATCAGGCAACTTTGAGCCAAACACAAGGAAGTGAAAAATTGTTGGTATCTTCAGCTCCAACACATCTG ACTCCCAATATTATTTTGTTAAATCAGACACCACTTTCTACACCACCAAATGTCAGTTCTTCACTTCCA AATCATATGCCCTCTTCAATCAATTTACTTGTGCAGAATCAGCAGACACCAAACAGTGCTATTTTAACA GGAAACAAGGCCAATGAAGAGGAGGAGGAGGAAATAATAGATGATGATGATGACACTATTAGCTCCAGT CCTGACTCGGCCGTCAGTAATACATCTTTGGTCCCACAGGCTGATACCTCCCAAAATACCAGTTTTGAT GGATCATTAATACAGAAGATGCAGATTCCTACACTTCTTCAAGAACCACTTTCCAATTCCTTAAAAATT TCAGATATAATTACTAGAAATACTAATGATCCAGGCGTAGGATCAAAACATCTAATGGAGGGTCAGAAG ATCATTACTTTAGATACAGCTACTGAAATTGAAGGCTTATCGACTGGTTGCAAGGTTTATGCAAATATC GGTGAAGATACTTATGATATAGTGATCCCTGTCAAAGATGACCCTGATGAAGGGGAGGCCAGACTTGAG AATGAAATACCAAAAACGTCTGGCAGCGAGATGGCAAACAAACGTATGAAAGTAAAACATGATGATCAC TATGAGTTAATAGTAGATGGAAGGGTCTATTATATCTGTATTGTATGCAAAAGGTCATATGTCTGTCTG ACAAGCTTGCGGAGACATTTTAACATTCATTCTTGGGAGAAGAAGTATCCGTGCCGTTACTGTGAGAAG GTATTTCCTCTTGCAGAATATCGCACAAAGCATGAAATTCATCACACAGGGGAGCGAAGGTATCAGTGT TTGGCCTGTGGCAAATCTTTCATCAACTATCAGTTTATGTCTTCACATATAAAGTCAGTTCATAGTCAA GATCCTTCTGGGGACTCAAAGCTTTATCGTTTACATCCATGCAGGTCTTTACAAATCAGACAATATGCA TATCTTTCCGATAGATCAAGCACTATTCCTGCAATGAAGGATGATGGTATTGGGTATAAGGTTGACACT GGAAAAGAACCTCCAGTAGGGACCACTACATCTACTCAGAACAAGCCAATGACCTGGGAAGATATTTTT ATTCAGCAGGAAAATGATTCAATTTTTAAACAAAATGTAACAGATGGCAGTACTGAGTTTGAATTTATA ATACCAGAGTCTTACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001184742 |
| Insert Size | 2019 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001184742.1 |
| RefSeq Size | 5324 bp |
| RefSeq ORF | 2019 bp |
| Locus ID | 10009 |
| UniProt ID | Q86T24 |
| MW | 74.5 kDa |
| Gene Summary | This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-α2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, May 2010] Transcript Variant: This variant (1) is the longer transcript. Variants 1 and 2 encode the same protein. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230416 | ZBTB33 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 33 (ZBTB33), transcript variant 1 |
CNY 4920.00 |
|
| RC230416L3 | Lenti ORF clone of Human zinc finger and BTB domain containing 33 (ZBTB33), transcript variant 1, Myc-DDK-tagged |
CNY 7320.00 |
|
| RC230416L4 | Lenti ORF clone of Human zinc finger and BTB domain containing 33 (ZBTB33), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG230416 | ZBTB33 (tGFP-tagged) - Human zinc finger and BTB domain containing 33 (ZBTB33), transcript variant 1 |
CNY 4370.00 |
