PPP2R1B (NM_181700) Human Untagged Clone
CAT＃: SC328972
PPP2R1B (untagged)-Human protein phosphatase 2 regulatory subunit A beta (PPP2R1B) transcript variant 3
CNY 9790.00
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CNY 1999.00
CNY 2700.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PP2A-Abeta; PR65B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328972 representing NM_181700.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGGCGCATCAGAGCTCGGGACCGGCCCAGGAGCAGCGGGTGGAGATGGAGATGATTCGCTATAC CCGATCGCGGTTTTAATCGACGAGCTCCGCAATGAAGACGTGCAGCCTCCTTTGGAAAATCTGGCAACT GTGGAAGAGACTGTTGTTCGTGACAAGGCTGTGGAGTCCCTGAGACAGATCTCCCAGGAGCATACTCCT GTTGCTCTGGAAGCTTATTTTGTACCTCTGGTGAAACGCTTAGCAAGTGGGGATTGGTTCACCTCTCGC ACATCTGCATGTGGTTTGTTCAGCGTTTGCTATCCCAGGGCATCAAATGCTGTTAAAGCAGAAATCAGA CAGCAATTCCGTTCCTTGTGCTCAGATGACACACCAATGGTACGACGTGCTGCTGCTTCCAAATTGGGT GAATTTGCAAAAGTTTTGGAATTAGACAGTGTGAAAAGTGAAATTGTTCCACTGTTCACTAGTCTAGCT TCAGATGAACAGGATTCAGTGCGCCTCCTTGCTGTGGAAGCTTGTGTCAGTATTGCCCAGTTATTGTCT CAGGATGACCTTGAGACTTTGGTGATGCCTACACTTCGACAAGCAGCAGAAGATAAATCTTGGCGCGTT CGCTATATGGTGGCTGACAGATTTTCAGAGCTCCAGAAAGCCATGGGTCCTAAAATCACCCTAAATGAC CTCATCCCCGCCTTTCAGAACCTACTTAAAGACTGTGAAGCTGAAGTCCGGGCAGCTGCTGCCCACAAA GTAAAAGAACTTGGTGAGAACTTGCCCATTGAAGATAGAGAGACCATAATTATGAATCAAATTCTGCCT TATATAAAGGAATTAGTATCCGATACCAATCAACATGTCAAATCGGCTCTAGCTTCTGTAATTATGGGA TTGTCTACTATTTTGGGCAAAGAAAATACCATTGAACATCTTCTACCTCTTTTCTTAGCTCAGTTAAAG GATGAGTGTCCTGACGTTCGTTTGAATATCATCTCCAATTTGGATTGTGTAAATGAAGTGATTGGAATC CGTCAGCTCTCTCAGTCTCTCCTTCCTGCCATAGTGGAGCTGGCAGAAGATGCCAAATGGAGGGTCCGC CTGGCCATCATTGAGTATATGCCGCTGCTGGCAGGCCAGCTGGGTGTGGAATTCTTTGATGAAAAGCTG AATTCTTTATGTATGGCTTGGCTCGTGGACCATGTATACGCCATCCGAGAAGCTGCCACCAACAACCTC ATGAAACTAGTTCAGAAGTTTGGTACAGAGTGGGCCCAAAATACTATTGTTCCCAAAGTGTTAGTAATG GCAAATGATCCTAATTACTTGCATAGAATGACCACTTTATTCTGCATTAATGCACTGTCTGAGGCCTGT GGTCAGGAAATAACTACTAAGCAAATGCTGCCCATCGTATTAAAAATGGCAGGAGACCAAGTAGCAAAT GTTCGCTTCAATGTGGCCAAATCTCTACAAAAGATTGGACCAATTCTAGATACCAATGCTTTACAGGGA GAAGTGAAGCCAGTACTACAGAAGTTAGGTCAAGATGAAGACATGGATGTCAAATACTTTGCACAGGAA GCTATAAGTGTGGTGGCACAAAGGCTGAGGAAGCTAGAATTTCCTGTGAAGGACAGTGGAGAACCCAGT GTCCCTCGGGCTGACAAGAACCACTTCCCAAGACCCACAGTGCCTGGAGAGGACATGGGGAAGGGACCA GTGTATCAGTTGCGTGGAGATACTAGAGACACACTTGCCCAGCTGGGAATTGCAGAGCTAGTGCATTTC TCCCAAAGCACAGACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_181700 |
| Insert Size | 1812 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_181700.1 |
| RefSeq Size | 1963 bp |
| RefSeq ORF | 1812 bp |
| Locus ID | 5519 |
| UniProt ID | P30154 |
| Protein Families | Druggable Genome, Phosphatase, Transcription Factors |
| Protein Pathways | Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway |
| MW | 66.6 kDa |
| Gene Summary | This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010] Transcript Variant: This variant (3) lacks two in-frame exons in the 5' coding region and differs in the 3' UTR and the 3' coding region, compared to variant 1. The resulting isoform (c) contains a longer and distinct C-terminus compared to isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230334 | PPP2R1B (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 3 |
CNY 4488.00 |
|
| RC230334L3 | Lenti-ORF clone of PPP2R1B (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 3 |
CNY 7220.00 |
|
| RC230334L4 | Lenti-ORF clone of PPP2R1B (mGFP-tagged)-Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 3 |
CNY 7220.00 |
|
| RG230334 | PPP2R1B (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 3 |
CNY 5890.00 |
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