BRIT1 (MCPH1) (NM_001172575) Human Untagged Clone
CAT＃: SC328918
MCPH1 (untagged)-Human microcephalin 1 (MCPH1) transcript variant 3
CNY 9030.00
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BRIT1; MCT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328918 representing NM_001172575.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGCCCCCATCCTGAAAGATGTAGTGGCCTATGTTGAAGTGTGGTCATCCAATGGAACAGAAAAT TATTCAAAGACATTTACAACACAGCTTGTGGATATGGGGGCAAAGGTTTCAAAAACTTTTAACAAACAA GTAACTCACGTTATCTTCAAAGATGGCTACCAGAGCACTTGGGACAAAGCTCAGAAGAGAGGCGTAAAG CTCGTTTCGGTGCTCTGGGTGGAAAAATGCAGGACAGCTGGAGCACACATTGATGAATCATTGTTCCCT GCAGCTAATATGAATGAACACTTATCAAGCCTAATTAAAAAAAAACGTAAATGTATGCAGCCCAAAGAT TTTAATTTTAAAACACCAGAAAATGATAAGAGATTTCAGAAGAAATTTGAGAAAATGGCTAAAGAGCTA CAAAGGCAAAAAACAAATCTAGCTTCCCAAATGATTCAGCAGTCTCATGATAATCCAAGTAACTCTCTG TGTGAAGCACCTTTGAACATTTCACGTGATACTTTGTGTTCAGATGAATACTTTGCTGGTGGCTTACAC TCATCTTTTGATGATCTTTGTGGAAACTCAGGATGTGGAAATCAGGAAAGGAAGTTGGAAGGATCCATT AATGACATTAAAAGTGATGTGTGTATTTCTTCACTTGTATTGAAAGCAAATAATATTCATTCATCACCA TCTTTCACTCACCTCGATAAATCAAGTCCTCAGAAATTTCTGAGTAATCTTTCAAAGGAAGAAATAAAC TTGCAAAGAAATATTGCAGGTAAAGTAGTCACCCCTGACCAAAAGCAGGCTGCAGGTATGTCTCAGGAG ACGTTTGAAGAGAAGTATCGTTTGTCTCCTACCTTATCTTCAACAAAAGGCCACCTTTTGATACATTCA AGACCCAGGAGTTCCTCAGTAAAGAGAAAAAGAGTATCACATGGCTCCCATTCACCTCCGAAGGAAAAA TGCAAGAGAAAGAGGAGCACCAGGAGATCTATCATGCCGAGGCTGCAGCTGTGCAGGTCGGAAGACAGG CTGCAGCACGTGGCGGGACCTGCCCTGGAGGCTCTTAGCTGTGGGGAGTCTTCATATGATGACTATTTT TCACCTGATAATCTTAAGGAAAGGTATTCAGAGAATCTTCCTCCTGAATCTCAGCTGCCATCAAGCCCT GCTCAGTTGAGCTGCAGAAGTCTTTCTAAGAAGGAGAGAACAAGCATATTTGAAATGTCTGATTTTTCC TGCGTTGGCAAAAAAACCAGAACAGTTGACATTACCAATTTCACAGCAAAAACCATCTCCAGTCCTCGG AAAACTGGAAATGGTGAAGGCCGTGCAACTTCGAGTTGCGTGACTTCTGCCCCTGAAGAAGCCCTAAGG TGTTGTAGACAGGCTGGGAAAGAAGACGCATGCCCAGAGGGAAATGGCTTTTCTTACACCATTGAGGAC CCTGCTCTTCCAAAAGGACATGATGATGATTTAACTCCTTTGGAAGGAAGCCTTGAAGAAATGAAAGAA GCGGTTGGTCTGAAAAGCACACAGAACAAAGGTACCACTTCCAAAATATCAAACTCCTCTGAAGGCGAA GCCCAGAGTGAACATGAGCCATGTTTTATAGTTGACTGTAACATGGAGACGTCTACAGAAGAGAAGGAA AACTTACCCGGAGGATACAGTGGAAGTATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001172575 |
| Insert Size | 1689 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001172575.1 |
| RefSeq Size | 3629 bp |
| RefSeq ORF | 1689 bp |
| Locus ID | 79648 |
| UniProt ID | Q8NEM0 |
| MW | 62.3 kDa |
| Gene Summary | This gene encodes a DNA damage response protein. The encoded protein may play a role in G2/M checkpoint arrest via maintenance of inhibitory phosphorylation of cyclin-dependent kinase 1. Mutations in this gene have been associated with primary autosomal recessive microcephaly 1 and premature chromosome condensation syndrome. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010] Transcript Variant: This variant (3) lacks an in-frame exon in the 5' coding region, and differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (3) has a distinct C-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230280 | MCPH1 (Myc-DDK-tagged)-Human microcephalin 1 (MCPH1), transcript variant 3 |
CNY 4184.00 |
|
| RC230280L3 | Lenti-ORF clone of MCPH1 (Myc-DDK-tagged)-Human microcephalin 1 (MCPH1), transcript variant 3 |
CNY 6840.00 |
|
| RC230280L4 | Lenti-ORF clone of MCPH1 (mGFP-tagged)-Human microcephalin 1 (MCPH1), transcript variant 3 |
CNY 6840.00 |
|
| RG230280 | MCPH1 (tGFP-tagged) - Human microcephalin 1 (MCPH1), transcript variant 3 |
CNY 5420.00 |
