Acyloxyacyl Hydrolase (AOAH) (NM_001177507) Human Untagged Clone
CAT＃: SC328891
AOAH (untagged)-Human acyloxyacyl hydrolase (neutrophil) (AOAH) transcript variant 3
CNY 8740.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328891 representing NM_001177507.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAGTCCCCCTGGAAAATCCTTACGGTGGCGCCTCTATTCTTGCTCCTGTCTCTTCAGTCCTCGGCC TCTCCAGCCAACGATGACCAGTCCAGGCCCAGCCTCTCGAATGGGCACACCTGTGTAGAAAAACTGTTC TTGAAAACCACCTGCTATTTAGTCATTGACAAGTTTGGATCAGACATCATAAAACTGCTTAGCGCAGAT ATGAATGCTGATGTGGTATGTCACACTCTGGAGTTTTGTAAACAGAACACTGGCCAACCATTGTGTCAT CTCTACCCTCTTCCCAAGGAGACATGGAAATTTACACTACAGAAGGCAAGACAAATTGTCAAGAAGTCC CCGATTCTGAAATATTCTAGAAGTGGTTCTGACATTTGTTCACTCCCGGTTTTGGCCAAGATCTGCCAG AAAATTAAATTAGCTATGGAACAGTCTGTGCCATTCAAAGATGTGGATTCAGACAAATACAGCGTTTTC CCAACACTGCGGGGCTATCACTGGCGGGGGAGAGACTGTAATGACAGCGACGAGTCAGTGTACCCAGGT AGAAGGCCGAACAACTGGGATGTCCATCAGGATTCAAACTGTAATGGCATTTGGGGTGTCGATCCAAAA GATGGAGTTCCATATGAGAAGAAATTCTGTGAAGGTTCACAGCCCAGGGGAATCATTTTGCTGGGAGAC TCAGCTGGGGCTCATTTTCACATCTCTCCTGAATGGATCACAGCGTCGCAGATGTCTTTGAACTCTTTC ATCAATCTACCAACAGCCCTTACCAACGAGCTTGACTGGCCCCAACTCTCTGGTGCTACAGGATTTCTG GACTCCACTGTTGGAATTAAAGAAAAATCTATTTACCTTCGCTTATGGAAAAGAAACCACTGTAATCAC AGGGACTACCAGAATATTTCAAGAAATGGTGCATCTTCCCGAAACCTGAAGAAATTTATAGAAAGCTTG TCTAGAAACAAGGTGTTGGACTATCCCGCCATCGTTATATATGCCATGATTGGAAATGATGTCTGCAGT GGGAAGAGTGACCCAGTCCCAGCCATGACCACTCCTGAGAAACTCTACTCCAACGTCATGCAGACTCTG AAGCATCTAAATTCCCACCTGCCCAATGGCAGCCATGTTATTTTGTATGGCTTACCAGATGGAACCTTT CTCTGGGATAATTTGCACAACAGATATCATCCTCTCGGCCAGCTAAATAAAGACATGACCTATGCGCAG TTGTACTCCTTCCTGAACTGCCTCCAGGTCAGCCCCTGCCACGGCTGGATGTCTTCCAACAAGACGTTG CGGACTCTCACTTCAGAGAGAGCAGAGCAACTCTCCAACACACTGAAAAAAATTGCAGCCAGTGAGAAA TTTACAAACTTCAATCTTTTCTACATGGATTTTGCCTTCCATGAAATCATACAGGAGTGGCAGAAGAGA GGCGGACAGCCCTGGCAGCTCATCGAGCCCGTGGATGGATTCCACCCCAACGAGGTGGCTTTGCTGTTG TTGGCGGATCATTTCTGGAAAAAGGTGCAGCTCCAGTGGCCCCAAATCCTGGGAAAGGAGAATCCGTTC AACCCCCAGATTAAACAGGTGTTTGGAGACCAAGGCGGGCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001177507 |
| Insert Size | 1632 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001177507.1 |
| RefSeq Size | 2344 bp |
| RefSeq ORF | 1632 bp |
| Locus ID | 313 |
| UniProt ID | P28039 |
| MW | 61.6 kDa |
| Gene Summary | This locus encodes both the light and heavy subunits of acyloxyacyl hydrolase. The encoded enzyme catalyzes the hydrolysis of acyloxylacyl-linked fatty acyl chains from bacterial lipopolysaccharides, effectively detoxifying these molecules. The encoded protein may play a role in modulating host inflammatory response to gram-negative bacteria. Alternatively spliced transcript variants have been described.[provided by RefSeq, Apr 2010] Transcript Variant: This variant (3) lacks an in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230253 | AOAH (Myc-DDK-tagged)-Human acyloxyacyl hydrolase (neutrophil) (AOAH), transcript variant 3 |
CNY 4040.00 |
|
| RC230253L1 | Lenti-ORF clone of AOAH (Myc-DDK-tagged)-Human acyloxyacyl hydrolase (neutrophil) (AOAH), transcript variant 3 |
CNY 6440.00 |
|
| RC230253L2 | Lenti-ORF clone of AOAH (mGFP-tagged)-Human acyloxyacyl hydrolase (neutrophil) (AOAH), transcript variant 3 |
CNY 6650.00 |
|
| RC230253L3 | Lenti-ORF clone of AOAH (Myc-DDK-tagged)-Human acyloxyacyl hydrolase (neutrophil) (AOAH), transcript variant 3 |
CNY 6650.00 |
|
| RC230253L4 | Lenti-ORF clone of AOAH (mGFP-tagged)-Human acyloxyacyl hydrolase (neutrophil) (AOAH), transcript variant 3 |
CNY 6650.00 |
|
| RG230253 | AOAH (tGFP-tagged) - Human acyloxyacyl hydrolase (neutrophil) (AOAH), transcript variant 3 |
CNY 5230.00 |
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