PPP1R3F (NM_001184745) Human Untagged Clone
CAT＃: SC328748
PPP1R3F (untagged)-Human protein phosphatase 1 regulatory (inhibitor) subunit 3F (PPP1R3F) transcript variant 2
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HB2E; LL0XNC01-7P3.1; R3F |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328748 representing NM_001184745.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGATGATAACACCTTTGCCATGGAGCATCCTGATGTCCAGGAGTCAGTGGGTCCACTGGTAGCCCCC ACCCCTCTCCGTCCATGGCCCCAGATGACACTTCAGGTTTCTGACGTTCCGATGACTGGCAACCCCGCA GAAGAAGGTGATGTCCCCAGAAGCAGTCCACCTGTGGCTTTTACAGAGGTCCTCCAGGCACCGGCCATC AGGATTCCCCCCTCCTCCCCTCTCTGTGGCCTGGGTGGCTCCCCCAGAGACCAGGCCTCAGGGCCCGAT GCGAGCGAGGGGGCCACCGGGCCTTTCCTGGAGCCCAGTCAGCAGCAGGCAGAGGCCACATGGGGAGTA TCGAGTGAGAATGGAGGGGGGCTGGAGGCTGTGAGTGGGTCAGAGGAGCTGCTCGGTGAGGACACCATC GACCAGGAGCTGGAGCAGCTCTACCTGTCTCACCTGAGCCGCCTACGGGCTGCTGTGGCTGCGGGTGGG GCAGGGGGTGGTGGGGAGGGCTCCACAGATGGAGGGATGTCCCCCAGCCATCCCCTGGGCATACTGACG GACCGCGACCTGATCTTGAAGTGGCCTGGCCCTGAGCGGGCCCTGAACAGCGCCCTGGCTGAGGAGATC ACGCTGCACTATGCCCGGCTGGGGCGTGGCGTGGAGCTCATCAAGGACACCGAAGACCCTGATGATGAA GGGGAGGGTGAAGAGGGGCTCTCTGTCACACCCTCCAGCCCAGAAGGGGACAGCCCCAAGGAATCGCCT CCAGAAATCCTCTCCGGGGCCCGTTCTGTGGTAGCCACGATGGGAGATGTGTGGCTCCCATGGGCAGAG GGCTCAGGATGTGACGGCCCTGTGGTTCTGGGTACAGAGGGTCAGTTCATTGGGGATCCTGAGAAAGGG ATGGGCAAGGACACCAGCTCTTTGCACATGAATAGGGTGATAGCTGGGGTGACTGAGTCCCTGGGGGAG GCCGGGACAGAAGCCCAGATAGAGGTCACCAGTGAGTGGGCAGGCAGCTTGGATCCCATATCTGGCAAG GAGCCAGCCTCTCCCGTCCTTCTGCAGGGGCAAAATCCCACCCTCCTCAGTCCCTTGGGGGCCGAAGTC TGTCTCTCTAGTGTAGCCAGGCCTCATGTGAGCTCCCAGGATGAAAAGGATGCAGGCCCAAGCCTTGAA CCCCCAAAGAAGTCTCCCACCCTAGCAGTCCCTGCAGAATGTGTGTGTGCACTGCCTCCTCAGCTCCGG GGGCCCTTGACCCAGACTCTGGGGGTCCTGGCCGGGCTAGTGGTGGTCCCTGTGGCTCTGAACAGCGGT GTGTCCCTCCTGGTGCTTGCGCTGTGCCTCTCTCTGGCTTGGTTCTCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001184745 |
| Insert Size | 1362 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001184745.1 |
| RefSeq Size | 2505 bp |
| RefSeq ORF | 1362 bp |
| Locus ID | 89801 |
| UniProt ID | Q6ZSY5 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 46.6 kDa |
| Gene Summary | This gene encodes a protein that has been identified as one of several type-1 protein phosphatase (PP1) regulatory subunits. One or two of these subunits, together with the well-conserved catalytic subunit, can form the PP1 holoenzyme, where the regulatory subunit functions to regulate substrate specificity and/or targeting to a particular cellular compartment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010] Transcript Variant: This variant (2) lacks a portion of the 5' coding region, uses a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (2) is shorter at the N-terminus and lacks an internal residue, compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230110 | PPP1R3F (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3F (PPP1R3F), transcript variant 2 |
CNY 3656.00 |
|
| RC230110L3 | Lenti-ORF clone of PPP1R3F (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3F (PPP1R3F), transcript variant 2 |
CNY 5890.00 |
|
| RC230110L4 | Lenti-ORF clone of PPP1R3F (mGFP-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3F (PPP1R3F), transcript variant 2 |
CNY 5890.00 |
|
| RG230110 | PPP1R3F (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 3F (PPP1R3F), transcript variant 2 |
CNY 4370.00 |
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