MPP1 (NM_001166462) Human Untagged Clone
CAT#: SC328722
MPP1 (untagged)-Human membrane protein palmitoylated 1 55kDa (MPP1) transcript variant 4
CNY 7030.00
Product images

Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | AAG12; DXS552E; EMP55; MRG1; PEMP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC328722 representing NM_001166462.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGTCCTGGGCTGTATCGCATCCATTGAATACTGTGACCGAGGACATGTACACCAACGGGTCTCCT GCCCCAGGTAGCCCTGCCCAGGTCAAGGGACAGGAGGTGCGGAAAGTGCGACTCATACAGTTTGAGAAG GTCACAGAAGAGCCCATGGGAATCACGCTGAAGCTGAATGAAAAACAGTCCTGTACGGTGGCCAGAATT CTTCATGGTGGCATGATCCATAGACAAGGCTCCCTTCACGTGGGGGATGAGATCCTAGAAATCAATGGC ACAAATGTGACAAATCATTCAGTGGATCAGCTGCAGAAGGCGATGAAAGAAACCAAAGGAATGATCTCA TTAAAAGTAATTCCCAACCAGCAAAGCCGTCTTCCTGCACTACAGATGTTCATGAGAGCGCAGTTTGAC TATGATCCCAAAAAGGACAATCTGATCCCTTGCAAGGAGGCGGGACTGAAGTTTGCTACTGGGGACATT ATCCAGATTATCAACAAGGATGACAGCAATTGGTGGCAGGGACGGGTGGAAGGCTCCTCCAAGGAGTCA GCAGGATTGATCCCTTCCCCTGAGCTGCAGGAATGGCGAGTGGCAAGTATGGCTCAGTCAGCTCCTAGC GAAGCCCCGAGCTGCAGTCCCTTTGGGAAGAAGAAGAAGTACAAAGACAAATATCTGGCCAAGCACAGC TCGATTTTTGATCAGTTGGATGTTGTTTCCTACGAGGAAGTCGTTCGGCTCCCTGCATTCAAGAGGAAG ACCCTGGTGCTGATCGGAGCCAGTGGGGTGGGTCGCAGCCACATTAAGAATGCCCTGCTCAGCCAGAAT CCGGAGAAGTTTGTGTACCCTGTCCCATATACAACACGGCCGCCAAGGAAGAGTGAGGAAGATGGGAAG GAGTACCACTTTATCTCAACGGAGGAGATGACGAGGAACATCTCTGCCAATGAGTTCTTGGAGTTTGGC AGCTACCAAGGCAACATGTTTGGCACCAAATTTGAAACAGTGCACCAGATCCATAAGCAGAACAAGATT GCCATCCTTGACATTGAGCCCCAGACCCTGAAAATTGTTCGGACAGCAGAACTTTCGCCTTTCATTGTG TTCATTGCACCTACTGACCAGGGCACTCAGACAGAAGCCCTGCAGCAGCTGCAGAAGGACTCTGAGGCC ATCCGCAGCCAGTACGCTCACTACTTTGACCTCTCACTGGTCAATAATGGTGTTGATGAAACCCTTAAG AAATTACAAGAAGCCTTCGACCAAGCGTGCAGTTCTCCACAGTGGGTGCCTGTCTCCTGGGTTTACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_001166462 |
Insert Size | 1311 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001166462.1 |
RefSeq Size | 2216 bp |
RefSeq ORF | 1311 bp |
Locus ID | 4354 |
UniProt ID | Q00013 |
Protein Families | Druggable Genome |
MW | 49.1 kDa |
Gene Summary | This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] Transcript Variant: This variant (4) contains an alternate exon in the 5' coding region that results in a distinct 5' UTR and translation initiation at an alternate start codon, compared to variant 1. The encoded protein (isoform 4) has a shorter and distinct N-terminus, compared to isoform 1. |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC230084 | MPP1 (Myc-DDK-tagged)-Human membrane protein, palmitoylated 1, 55kDa (MPP1), transcript variant 4 |
CNY 3656.00 |
|
RC230084L3 | Lenti-ORF clone of MPP1 (Myc-DDK-tagged)-Human membrane protein, palmitoylated 1, 55kDa (MPP1), transcript variant 4 |
CNY 5890.00 |
|
RC230084L4 | Lenti-ORF clone of MPP1 (mGFP-tagged)-Human membrane protein, palmitoylated 1, 55kDa (MPP1), transcript variant 4 |
CNY 5890.00 |
|
RG230084 | MPP1 (tGFP-tagged) - Human membrane protein, palmitoylated 1, 55kDa (MPP1), transcript variant 4 |
CNY 4370.00 |