BRUNOL5 (CELF5) (NM_001172673) Human Untagged Clone
CAT#: SC328662
CELF5 (untagged)-Human CUGBP Elav-like family member 5 (CELF5) transcript variant 2
CNY 6650.00
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | BRUNOL-5; BRUNOL5; CELF-5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC328662 representing NM_001172673.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCCGCCTGACGGAGAGCGAGGCGCGCCGGCAGCAGCAGCAGCTCCTGCAGCCGCGGCCCTCGCCC GTGGGCAGCAGCGGGCCCGAGCCCCCCGGGGGGCAGCCCGACGGCATGAAGGACCTGGACGCCATCAAA CTCTTCGTGGGCCAGATCCCGCGGCACCTGGACGAGAAGGACCTCAAGCCGCTCTTCGAGCAGTTCGGC CGCATCTACGAGCTCACGGTGCTCAAAGACCCCTACACGGGGATGCACAAAGGCTGTGCCTTCCTCACC TACTGTGCCAGGGATTCCGCCATCAAAGCTCAGACTGCCCTGCACGAGCAGAAGACCTTGCCCGGAATG GCGCGGCCAATCCAGGTGAAGCCTGCGGACAGTGAAAGCCGCGGAGGTAGGGACCGGAAGCTGTTCGTG GGGATGCTGAACAAGCAGCAGTCGGAGGAGGACGTGCTGCGGCTGTTCCAGCCCTTCGGGGTCATTGAC GAGTGCACCGTGCTCCGGGGGCCTGACGGCAGCAGCAAAGGCTGTGCTTTCGTGAAGTTCTCCTCCCAC ACGGAGGCGCAGGCGGCCATCCACGCCTTGCATGGGAGCCAGACCATGCCGGGAGCCTCCTCCAGCCTG GTGGTCAAGTTCGCCGACACGGACAAGGAGCGGACGCTCCGGCGCATGCAGCAGATGGTGGGCCAGCTG GGCATCCTGACGCCGTCCCTCACATTGCCCTTCAGCCCCTACAGTGCCTACGCCCAGGCTCTCATGCAA CAGCAGACAACAGTCCTGTCCACCTCGGGCAGCTACCTGAGTCCCGGCGTGGCCTTCTCACCCTGTCAC ATCCAGCAGATAGGCGCCGTCAGCCTCAACGGGCTGCCTGCCACACCCATCGCTCCTGCCTCTGGTGTC GTGCCCTTTCCAGGTGGGCACCCTGCCCTGGAAACCGTCTATGCCAATGGCCTTGTGCCCTACCCAGCT CAGAGCCCGACTGTGGCCGAGACACTGCATCCTGCCTTCTCCGGAGTCCAGCAGTACACAGCCATGTAC CCCACCGCGGCCATCACGCCCATCGCGCACAGCGTCCCCCAGCCGCCGCCCCTCCTGCAGCAGCAGCAG CGAGAAGGAGTTTGGAGACACGGAGCTGACGCAGATGTTCCTACCCTTCGGCAATATCATTTCCTCCAA GGTGTTTATGGATCGAGCTACCAACCAGAGCAAGTGTTTCGGCTTCGTGAGCTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_001172673 |
Insert Size | 1230 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001172673.1 |
RefSeq Size | 5008 bp |
RefSeq ORF | 1230 bp |
Locus ID | 60680 |
UniProt ID | Q8N6W0 |
MW | 44.5 kDa |
Gene Summary | This gene encodes a member of the the CELF/BRUNOL protein family, which contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing and translation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (2) contains an alternate in-frame splice site in the central coding region, an alternate splice site that results in a frameshift in the 3' coding region, and it includes an additional segment in the 3' UTR, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC230024 | CELF5 (Myc-DDK-tagged)-Human CUGBP, Elav-like family member 5 (CELF5), transcript variant 2 |
CNY 3656.00 |
|
RC230024L3 | Lenti ORF clone of Human CUGBP, Elav-like family member 5 (CELF5), transcript variant 2, Myc-DDK-tagged |
CNY 5890.00 |
|
RC230024L4 | Lenti ORF clone of Human CUGBP, Elav-like family member 5 (CELF5), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
RG230024 | CELF5 (tGFP-tagged) - Human CUGBP, Elav-like family member 5 (CELF5), transcript variant 2 |
CNY 4370.00 |
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