JMY (NM_152405) Human Untagged Clone
CAT＃: SC328036
JMY (untagged)-Human junction mediating and regulatory protein p53 cofactor (JMY)
CNY 19000.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | WHAMM2; WHDC1L3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328036 representing NM_152405.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGTTCGCGCTGGAGGAGACGCTCGAGTCGGACTGGGTGGCTGTGCGGCCCCATGTGTTCGACGAG CGCGAGAAACACAAATTCGTCTTTATTGTGGCCTGGAACGAGATTGAGGGCAAGTTTGCCATAACCTGC CACAACCGGACGGCCCAGAGGCAGAGGAGCGGCTCCCGGGAGCAAGCGGGGGCGCGAGGGGGCGCCGAG GCCGGCGGAGCTGCGTCCGACGGGAGCCGCGGGCCCGGCAGCCCGGCGGGCAGGGGTCGGCCCGAGGCC ACTGCCTCTGCAACTCTGGTTAGGAGCCCCGGGCCCCGGCGGAGCTCGGCCTGGGCGGAGGGCGGCTCT CCTCGGAGCACTCGCAGCCTTCTGGGGGACCCGCGGCTGCGGAGTCCTGGCAGCAAAGGGGCGGAGAGT CGTCTTAGGAGCCCAGTGCGGGCCAAACCCATCCCGGGTCAGAAAACATCTGAAGCCGACGATGCGGCG GGGGCAGCCGCTGCAGCAGCCCGGCCGGCGCCCAGAGAGGCCCAGGTGTCCTCTGTACGGATAGTGAGC GCCTCTGGGACGGTCTCCGAGGAGATAGAGGTGCTGGAAATGGTGAAGGAGGACGAGGCACCTCTGGCG CTCTCGGACGCGGAGCAGCCGCCGCCCGCCACCGAGCTGGAGTCTCCGGCCGAAGAGTGCAGCTGGGCC GGACTGTTTTCTTTCCAGGACCTGCGCGCCGTGCACCAGCAGCTGTGCTCGGTGAACTCGCAGTTGGAG CCGTGCCTGCCGGTGTTCCCCGAGGAACCTTCGGGCATGTGGACTGTGCTGTTTGGGGGCGCCCCCGAG ATGACCGAGCAGGAAATCGACACTCTGTGTTACCAGCTCCAGGTCTACCTGGGCCACGGCCTGGACACC TGCGGCTGGAAGATCCTCTCCCAGGTGCTCTTCACCGAGACCGATGATCCCGAGGAGTATTACGAAAGC CTCAGCGAGCTGCGGCAGAAGGGCTACGAAGAAGTGCTTCAGCGGGCCAGGAAGCGCATCCAGGAGCTC TTGGATAAGCACAAGAATACAGAGAGCATGGTGGAGCTTCTGGACTTGTATCAGATGGAGGATGAAGCC TACAGCAGCCTTGCAGAAGCTACAACCGAACTCTATCAGTATTTACTACAGCCATTCCGAGACATGAGA GAACTTGCCATGCTACGAAGACAGCAGATCAAGATTTCCATGGAGAATGATTATCTGGGACCTCGAAGA ATTGAGAGTCTACAAAAAGAAGATGCTGATTGGCAGCGGAAAGCTCACATGGCTGTACTGTCTATTCAA GATCTTACTGTCAAGTACTTTGAAATAACAGCTAAAGCTCAAAAAGCTGTGTATGATCGAATGCGAGCT GATCAGAAGAAATTTGGTAAAGCATCATGGGCAGCGGCTGCTGAACGGATGGAAAAACTCCAGTATGCA GTTTCTAAGGAAACTTTGCAGATGATGAGAGCTAAAGAGATATGCTTGGAACAGCGGAAACATGCACTA AAGGAAGAGATGCAGAGTTTGCGGGGTGGTACAGAAGCGATAGCACGATTGGATCAGTTAGAAGCTGAT TATTATGATCTGCAACTTCAGTTGTATGAAGTACAGTTTGAAATCTTGAAGTGTGAAGAGTTACTATTG ACAGCGCAACTAGAAAGCATCAAAAGACTTATATCAGAAAAAAGAGATGAAGTGGTATACTATGACACT TACGAAAGCATGGAGGCCATGCTGGAGAAGGAAGAGATGGCAGCATCTGCGTACTTACAGAGAGAAGAG CTGCAGAAACTTCAGCAGAAAGCACGCCAGCTGGAAGCAAGACGTGGACGGGTTTCTGCCAAGAAATCC TACCTCAGAAATAAAAAGGAAATATGTATTGCAAAACACAATGAAAAAATCCAACAGCGCACTCGGATT GAAGATGAATATAGAACCCATCACACAGTACAACTAAAGAGAGAAAAATTACATGATGAAGAAGAAAGA AAAAGTGCCTGGGTTAGCCAAGAGAGACAGAGAACACTGGATAGACTTCGAACATTTAAACAGAGGTAT CCTGGGCAAGTCATACTTAAATCAACCAGATTACGACTAGCTCATGCAAGAAGAAAAGGTGCAGCAAGT CCTGTTCTCCAAGAGGATCATTGTGACTCTTTACCAAGTGTGTTACAGGTAGAAGAGAAAACTGAAGAG GTGGGAGAAGGAAGAGTCAAGCGTGGGCCATCACAGACAACAGAACCCCAGAGCCTTGTGCAACTTGAA GATACTTCATTAACACAACTTGAAGCCACCTCATTACCTCTCAGTGGTGTTACCTCTGAACTGCCTCCC ACTATATCTCTTCCACTTTTGAATAACAACCTCGAACCATGTTCTGTTACCATAAATCCACTCCCATCC CCTCTTCCTCCAACACCACCACCTCCCCCACCTCCTCCCCCTCCCCCACCACCACCACCTCTGCCTGTT GCTAAGGACAGTGGCCCAGAGACACTGGAGAAAGATCTGCCTAGAAAGGAGGGGAATGAGAAGAGGATC CCAAAGTCAGCCAGTGCCCCCTCAGCACACCTCTTTGACAGCAGCCAGCTGGTCAGTGCACGGAAGAAG CTCAGAAAGACTGCTGAAGGTTTGCAGAGGAGGAGAGTGAGCTCACCCATGGATGAGGTGCTAGCCTCC TTGAAGCGTGGTAGTTTTCATCTGAAAAAGGTTGAACAGCGAACTCTGCCTCCTTTTCCTGATGAAGAT GATAGTAATAATATCTTGGCACAAATAAGGAAAGGGGTAAAATTGAAGAAGGTACAGAAGGATGTTTTG AGAGAATCCTTCACACTTCTACCCGATACAGACCCTCTAACACGGAGCATCCATGAAGCTCTTAGAAGA ATTAAAGAAGCATCCCCAGAGTCAGAGGACGAAGAGGAGGCTTTACCTTGCACAGACTGGGAGAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_152405 |
| Insert Size | 2967 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_152405.4 |
| RefSeq Size | 9145 bp |
| RefSeq ORF | 2967 bp |
| Locus ID | 133746 |
| UniProt ID | Q8N9B5 |
| MW | 111.4 kDa |
| Gene Summary | Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229401 | JMY (Myc-DDK-tagged)-Human junction mediating and regulatory protein, p53 cofactor (JMY) |
CNY 7240.00 |
|
| RC229401L3 | Lenti ORF clone of Human junction mediating and regulatory protein, p53 cofactor (JMY), Myc-DDK-tagged |
CNY 10640.00 |
|
| RC229401L4 | Lenti ORF clone of Human junction mediating and regulatory protein, p53 cofactor (JMY), mGFP tagged |
CNY 10640.00 |
|
| RG229401 | JMY (tGFP-tagged) - Human junction mediating and regulatory protein, p53 cofactor (JMY) |
CNY 9600.00 |
|
| SC318196 | JMY (untagged)-Human junction mediating and regulatory protein, p53 cofactor (JMY) |
CNY 19000.00 |
