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TMEM16A (ANO1) (NM_018043) Human Untagged Clone
CAT＃: SC328035
ANO1 (untagged)-Human anoctamin 1 calcium activated chloride channel (ANO1)
CNY 19,000.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DOG1; ORAOV2; TAOS2; TMEM16A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC328035 representing NM_018043.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGGGTCAACGAGAAGTACTCGACGCTCCCGGCCGAGGACCGCAGCGTCCACATCATCAACATCTGC GCCATCGAGGACATCGGCTACCTGCCGTCCGAGGGCACGCTGCTGAACTCCTTATCTGTGGACCCTGAT GCCGAGTGCAAGTATGGCCTGTACTTCAGGGACGGCCGGCGCAAGGTGGACTACATCCTGGTGTACCAT CACAAGAGGCCCTCGGGCAACCGGACCCTGGTCAGGAGGGTGCAGCACAGCGACACCCCCTCTGGGGCT CGCAGCGTCAAGCAGGACCACCCCCTGCCGGGCAAGGGGGCGTCGCTGGATGCAGGCTCGGGGGAGCCC CCGATGGACTACCACGAGGATGACAAGCGCTTCCGCAGGGAGGAGTACGAGGGCAACCTCCTGGAGGCG GGCCTGGAGCTGGAGCGGGACGAGGACACTAAAATCCACGGAGTCGGGTTTGTGAAAATCCATGCCCCC TGGAACGTGCTGTGCAGAGAGGCCGAGTTTCTGAAACTGAAGATGCCGACGAAGAAGATGTACCACATT AATGAGACCCGTGGCCTCCTGAAAAAAATCAACTCTGTGCTCCAGAAAATCACAGATCCCATCCAGCCC AAAGTGGCTGAGCACAGGCCCCAGACCATGAAGAGACTCTCCTATCCCTTCTCCCGGGAGAAGCAGCAT CTATTTGACTTGTCTGATAAGGATTCCTTTTTCGACAGCAAAACCCGGAGCACGATTGTCTATGAGATC TTGAAGAGAACGACGTGTACAAAGGCCAAGTACAGCATGGGCATCACGAGCCTGCTGGCCAATGGTGTG TACGCGGCTGCATACCCACTGCACGATGGAGACTACAACGGTGAAAACGTCGAGTTCAACGACAGAAAA CTCCTGTACGAAGAGTGGGCACGCTATGGAGTTTTCTATAAGTACCAGCCCATCGACCTGGTCAGGAAG TATTTTGGGGAGAAGATCGGCCTGTACTTCGCCTGGCTGGGCGTGTACACCCAGATGCTCATCCCTGCC TCCATCGTGGGAATCATTGTCTTCCTGTACGGATGCGCCACCATGGATGAAAACATCCCCAGCATGGAG ATGTGTGACCAGAGACACAATATCACCATGTGCCCGCTTTGCGACAAGACCTGCAGCTACTGGAAGATG AGCTCAGCCTGCGCCACGGCCCGCGCCAGCCACCTCTTCGACAACCCCGCCACGGTCTTCTTCTCTGTC TTCATGGCCCTCTGGGCTGCCACCTTCATGGAGCACTGGAAGCGGAAACAGATGCGACTCAACTACCGC TGGGACCTCACGGGCTTTGAAGAGGAAGAGGAGGCTGTCAAGGATCATCCTAGAGCTGAATACGAAGCC AGAGTCTTGGAGAAGTCTCTGAAGAAAGAGTCCAGAAACAAAGAGAAGCGCCGGCATATTCCAGAGGAG TCAACAAACAAATGGAAGCAGAGGGTTAAGACAGCCATGGCGGGGGTGAAATTGACTGACAAAGTGAAG CTGACATGGAGAGATCGGTTCCCAGCCTACCTCACTAACTTGGTCTCCATCATCTTCATGATTGCAGTG ACGTTTGCCATCGTCCTCGGCGTCATCATCTACAGAATCTCCATGGCCGCCGCCTTGGCCATGAACTCC TCCCCCTCCGTGCGGTCCAACATCCGGGTCACAGTCACAGCCACCGCAGTCATCATCAACCTAGTGGTC ATCATCCTCCTGGACGAGGTGTATGGCTGCATAGCCCGATGGCTCACCAAGATCGAGGTCCCAAAGACG GAGAAAAGCTTTGAGGAGAGGCTGATCTTCAAGGCTTTCCTGCTGAAGTTTGTGAATTCCTACACCCCC ATCTTTTACGTGGCGTTCTTCAAAGGCCGGTTTGTTGGACGCCCGGGCGACTACGTGTACATTTTCCGT TCCTTCCGAATGGAAGAGTGTGCGCCAGGGGGCTGCCTGATGGAGCTATGCATCCAGCTCAGCATCATC ATGCTGGGGAAACAGCTGATCCAGAACAACCTGTTCGAGATCGGCATCCCGAAGATGAAGAAGCTCATC CGCTACCTGAAGCTGAAGCAGCAGAGCCCCCCTGACCACGAGGAGTGTGTGAAGAGGAAACAGCGGTAC GAGGTGGATTACAACCTGGAGCCCTTCGCGGGCCTCACCCCAGAGTACATGGAAATGATCATCCAGTTT GGCTTCGTCACCCTGTTTGTCGCCTCCTTCCCCCTGGCCCCACTGTTTGCGCTGCTGAACAACATCATC GAGATCCGCCTGGACGCCAAAAAGTTTGTCACTGAGCTCCGAAGGCCGGTAGCTGTCAGAGCCAAAGAC ATCGGAATCTGGTACAATATCCTCAGAGGCATTGGGAAGCTTGCTGTCATCATCAATGCCTTCGTGATC TCCTTCACGTCTGACTTCATCCCGCGCCTGGTGTACCTCTACATGTACAGTAAGAACGGGACCATGCAC GGCTTCGTCAACCACACCCTCTCCTCCTTCAACGTCAGTGACTTCCAGAACGGCACGGCCCCCAATGAC CCCCTGGACCTGGGCTACGAGGTGCAGATCTGCAGGTATAAAGACTACCGAGAGCCGCCGTGGTCGGAA AACAAGTACGACATCTCCAAGGACTTCTGGGCCGTCCTGGCAGCCCGGCTGGCGTTTGTCATCGTCTTC CAGAACCTGGTCATGTTCATGAGCGACTTTGTGGACTGGGTCATCCCGGACATCCCCAAGGACATCAGC CAGCAGATCCACAAGGAGAAGGTGCTCATGGTGGAGCTGTTCATGCGGGAGGAGCAAGACAAGCAGCAG CTGCTGGAAACCTGGATGGAGAAGGAGCGGCAGAAGGACGAGCCGCCGTGCAACCACCACAACACCAAA GCCTGCCCAGACAGCCTCGGCAGCCCAGCCCCCAGCCATGCCTACCACGGGGGCGTCCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_018043 |
| Insert Size | 2961 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018043.5 |
| RefSeq Size | 4811 bp |
| RefSeq ORF | 2961 bp |
| Locus ID | 55107 |
| UniProt ID | Q5XXA6 |
| Domains | DUF590 |
| Protein Families | Transmembrane |
| MW | 114.1 kDa |
| Gene Summary | Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
TMEM16A, A Membrane Protein Associated With Calcium-Dependent Chloride Channel Activity
,Caputo A, Caci E, Ferrera L, Pedemonte N, Barsanti C, Sondo E, Pfeffer U, Ravazzolo R, Zegarra-Moran O, Galietta LJ.,
Science. 2008 Sep 4. [Epub ahead of print]
[ANO1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213098 | ANO1 (Myc-DDK-tagged)-Human anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1 |
CNY 10,544.00 |
|
| RC213098L3 | Lenti-ORF clone of ANO1 (Myc-DDK-tagged)-Human anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1 |
CNY 11,690.00 |
|
| RC213098L4 | Lenti-ORF clone of ANO1 (mGFP-tagged)-Human anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1 |
CNY 11,690.00 |
|
| RC229400 | ANO1 (Myc-DDK-tagged)-Human anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1 |
CNY 7,224.00 |
|
| RC229400L1 | Lenti-ORF clone of ANO1 (Myc-DDK-tagged)-Human anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1 |
CNY 9,624.00 |
|
| RC229400L2 | Lenti-ORF clone of ANO1 (mGFP-tagged)-Human anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1 |
CNY 9,624.00 |
|
| RC229400L3 | Lenti-ORF clone of ANO1 (Myc-DDK-tagged)-Human anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1 |
CNY 9,624.00 |
|
| RC229400L4 | Lenti-ORF clone of ANO1 (mGFP-tagged)-Human anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1 |
CNY 9,624.00 |
|
| RG213098 | ANO1 (tGFP-tagged) - Human anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1 |
CNY 12,144.00 |
|
| RG229400 | ANO1 (tGFP-tagged) - Human anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1 |
CNY 10,740.00 |
|
| SC316851 | ANO1 (untagged)-Human anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1 |
CNY 10,552.00 |
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