KIAA1549 (NM_001164665) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RP86
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC327338 representing NM_001164665. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCGGGGGCGCGGCGCCGACGCCGAGGCGCGGCCATGGAGGGGAAGCCCCGCGCCGGGGTCGCGCTG GCCCCGGGGCCGAGCGGCCGACGGCCTTCCGCCCGCTGCGCCCGCCGCCGCCGCCCGGGGCTGCTGCTT CCTGGCCTCTGGCTGCTGCTGCTGGCCCGGCCGGCCTCGTGCGCCCCAGATGAGCTCTCTCCGGAACAG CACAACCTTTCTTTATACTCCATGGAGCTCGTGCTGAAGAAAAGCACTGGGCACAGCGCTGCACAAGTG GCCTTAACAGAAACTGCTCCCGGCTCCCAGCACAGCAGTCCTCTCCATGTCACAGCCCCGCCGTCTGCC ACTACTTTTGATACAGCCTTTTTTAACCAAGGAAAACAGACCAAAAGTACAGCAGATCCCAGCATCTTT GTGGCAACTTACGTGTCAGTGACGAGTAAAGAGGTGGCCGTCAATGACGATGAGATGGATAACTTTCTG CCAGATACTCACTGGACCACTCCACGGATGGTTTCTCCAATACAGTATATCACAGTCAGCCCACCAGGG CTGCCCAGGGAAGCATTAGAACCTATGCTCACTCCATCATTACCCATGGTTTCTTTACAAGATGAAGAA GTGACATCGGGCTGGCAGAACACAACGCGACAACCAGCGGCATATGCTGAGTCCGCCAGTCATTTCCAC ACCTTTCGGTCAGCTTTTCGCACCTCTGAGGGCATCGTTCCAACTCCTGGCAGGAATTTGGTGCTTTAT CCTACTGATGCTTACAGTCATTTATCAAGCAGGACTCTGCCAGAGATTGTGGCTTCCCTAACAGAGGGT GTGGAAACCACCCTTTTTTTAAGCTCCCGGTCTTTAATGCCACAGCCGTTAGGCGACGGCATTACTATA CCGTTGCCCTCCTTGGGGGAGGTCTCACAGCCTCCAGAGGAGGTTTGGGCCACAAGTGCAGACAGATAC ACTGATGTGACCACTGTGTTGAGTCAAAGCCTAGAAGAAACCATCTCTCCAAGAACATACCCCACTGTG ACTGCATCGCACGCAGCCCTTGCATTCAGCAGGACACATTCTCCATTGCTTTCAACTCCTCTTGCATTT GCGTCCTCTGCTTCACCAACTGATGTTTCATCTAACCCCTTTCTCCCTAGCGACTCCAGCAAAACATCC GAATTGCATAGCAATTCAGCCCTCCCCGGTCCTGTGGACAACACTCATATCCTGAGCCCGGTGTCCTCA TTCAGACCATACACTTGGTGTGCGGCCTGCACTGTGCCTTCACCTCAGCAAGTTCTGGCCACGAGCCTC ATGGAGAAAGACGTGGGATCAGGGGATGGTGCCGAGACTCTGTGCATGACCGTGCTGGAAGAAAGCAGC ATCTCTCTAATGAGTAGCGTCGTAGCAGACTTCTCTGAATTTGAGGAAGATCCTCAAGTATTTAATACG CTTTTCCCCTCCAGACCTATCGTCCCACTTTCTTCTAGATCCATGGAAATCTCAGAGACGAGTGTTGGC ATTTCTGCCGAGGTGGATATGAGTAGTGTTACAACCACACAGGTTCCCCCTGCCCACGGCCGCCTCTCT GTGCCGGCGTCACTTGATCCTACTGCTGGCTCCTTGTCTGTTGCTGAAACCCAAGTGACGCCATCCAGC GTGACCACTGCATTTTTCTCGGTCATCACCAGCATTCTCCTTGACTCATCTTTCTCTGTCATAGCAAAC AAAAACACACCGTCGCTTGCCGTCAGAGACCCGAGTGTTTTTACGCCTTATAGTCTGGTTCCTTCAGTG GAGTCTTCACTTTTCTCTGACCAAGAACGTTCCAGTTTTTCTGAGCATAAACCCAGAGGTGCTTTGGAT TTTGCATCCAGCTTTTTCTCAACACCCCCGCTGGAACTCAGCGGCTCCATCTCTTCGCCTTCGGAAGCA CCTGCGTCTCTGTCTCTGATGCCGAGTGACTTGTCCCCCTTCACATCTCAGTCTTTTTCTCCCTTGGTT GAGACATTTACATTGTTTGACTCTAGTGATCTGCAGTCATCTCAGCTGTCTCTTCCCAGTTCCACAAAT CTTGAGTTTTCGCAGCTCCAGCCAAGTTCCGAGCTGCCTTTAAACACCATCATGTTGCTACCTAGCCGT TCTGAGGTGTCACCATGGTCAAGCTTCCCTTCTGATTCTCTCGAGTTTGTTGAAGCGTCTACGGTTTCA CTGACGGATTCAGAAGCTCATTTTACCTCAGCTTTCATTGAAACTACCTCCTATCTTGAGTCTTCACTC ATTTCCCATGAATCCGCAGTCACTGCACTGGTGCCCCCCGGCTCTGAGTCTTTTGACATTTTGACTGCC GGGATTCAAGCAACATCACCATTGACCACTGTCCACACAACGCCCATTTTAACTGAGTCTTCTTTGTTC TCAACTCTGACACCTCCTGACGACCAAATCAGTGCTCTAGACGGTCACGTGTCTGTCCTGGCCTCTTTC TCCAAAGCCATTCCCACTGGTACGGTGTTGATCACTGACGCGTACCTGCCATCAGGATCCTCGTTTGTT TCTGAAGCAACCCCCTTCCCTCTGCCCACAGAGCTGACCGTCGTGGGCCCATCACTCACACCCACAGAG GTGCCACTGAACACCTCCACGGAAGTGAGCACAACCAGCACCGGTGCTGCCACTGGTGGTCCCCTCGAC TCCACCCTGATGGGTGACGCCGCAAGTCAGAGCCCCCCAGAGAGTAGTGCTGCTCCTCCCCTGCCATCC CTGCGTCCCGTGACTGCCTTCACTCTCGAAGCAACAGTCGACACACCAACACTGGCTACTGCCAAGCCG CCATATGTTTGTGATATCACAGTCCCCGATGCCTATCTGATCACAACTGTGCTGGCCAGAAGAGCTGTG CAGGAGTACATCATTACAGCAATCAAAGAAGTACTGAGGATTCACTTCAACCGTGCAGTGGAACTGAAG GTTTACGAACTATTTACTGACTTCACTTTTCTGGTAACATCCGGTCCTTTCGTTTACACGGCAATATCC GTCATAAATGTGCTTATAAACAGTAAGCTTGTCCGTGACCAGACTCCTTTAATCCTGTCTGTGAAACCT TCTTTCCTTGTGCCAGAGTCCAGGTTCCAAGTTCAAACAGTACTTCAGTTTGTGCCTCCGAGTGTGGAT ACTGGCTTCTGCAACTTCACCCAGCGCATTGAGAAAGGCCTAATGACAGCTCTCTTTGAAGTGAGAAAA CACCACCAGGGAACGTATAACCTCACGGTGCAGATCTTGAATATCACCATCAGTTCCTCAAGGGTGACT CCTCGGCGGGGCCCGGTGAATATCATCTTTGCGGTTAAAAGCACACAGGGATTTTTGAATGGGTCGGAA GTGAGCGAGCTGCTCAGAAACTTGAGTGTGGTGGAGTTCAGTTTCTATCTGGGATACCCAGTGCTGCAG ATCGCAGAGCCCTTCCAGTATCCACAGCTCAACTTATCTCAGTTGCTGAAGTCCTCTTGGGTCAGAACA GTTCTCCTGGGCGTCATGGAGAAGCAACTCCAGAATGAAGTGTTTCAAGCCGAGATGGAACGCAAGCTG GCCCAGCTGCTCAGCGAGGTTTCCACCAGAAGGCGGATGTGGAGAAGGGCCACTGTAGCTGCAGGGAAC AGTGTGGTGCAGGTGGTAAATGTGTCGAGGCTGGAGGGAGATGACAATCCGGTACAGCTCATCTACTTT GTGGAGGATCAAGATGGAGAAAGACTCAGTGCAGTCAAGTCTTCGGACCTGATTAACAAAATGGACCTC CAGAGAGCAGCCATCATCTTGGGTTACCGAATTCAAGGTGTCATTGCCCAGCCTGTCGACAGGGTGAAG AGGCCGTCTCCGGAATCCCAGAGCAACAACTTGTGGGTCATTGTTGGCGTGGTCATCCCAGTGCTGGTG GTGATGGTGATTGTTGTCATCCTCTACTGGAAACTATGCCGCACAGACAAGCTAGACTTTCAGCCTGAC ACTGTGGCCAACATTCAGCAGCGTCAGAAGCTGCAGATCCCTAGTGTGAAGGGCTTCGATTTTGCTAAG CAGCATCTGGGTCAGCACAATAAAGACGACATATTGATTATTCATGAGCCAGCGCCACTGCCAGGACCT CTGAAGGACCACACCACGCCCTCGGAAAATGGAGACGTGCCAAGCCCCAAGTCAAAGATCCCTTCCAAG AATGTTCGTCACAGAGGAAGAGTTTCTCCCTCAGATGCTGACTCTACGGTCAGTGAAGAGTCCAGCGAG AGGGACGCAGGAGATAAGACGCCGGGAGCCGTCAACGATGGCAGGTCCCACAGAGCTCCGCAGAGCGGG CCACCACTGCCCAGTTCGGGAAATGAGCAGCACTCATCAGCCTCCATCTTCGAGCACGTGGACAGGATC TCCCGCCCCCCGGAGGCTAGCCGGCGGGTCCCCAGTAAGATCCAGCTTATCGCCATGCAGCCGATCCCG GCACCTCCCGTCCAGCGCCCCTCCCCAGCCGACCGAGTGGCGGAAAGCAATAAAATCAACAAAGAGATT CAGACCGCGCTGCGGCACAAGTCTGAGATCGAGCACCATCGCAACAAGATCCGCCTGCGCGCCAAGCGC CGCGGGCACTACGAGTTCCCGGTGGTAGACGACCTGTCCTCGGGCGACACTAAGGAGCGACACCGGGTG TACCGCAGGGCACAGATGCAGATCGACAAGATCCTGGACCCCACGGCCAGCGTGCCCTCCGTGTTCATA GAGCCCAGGAAGAGCTCACGGATAAAACGTTCTCCCAAGCCTCGCCGGAAACACCAGGTCAACGGCTGT CCTGCCGACGCTGAGAAGGACCGGCTCATCACCACAGACAGCGATGGCACCTACAGGAGGCCCCCCGGC GTCCACAACTCAGCCTACATCGGATGCCCATCGGATCCTGACCTCCCAGCCGATGTGCAGACACCATCC TCGGTGGAACTGGGGAGGTATCCAGCCCTTCCCTTCCCGGCCTCCCAGTACATCCCACCCCAGCCGTCC ATCGAGGAGGCACGCCAGACCATGCACTCCCTCCTGGACGACGCCTTTGCCCTCGTGGCCCCCAGCAGC CAGCCTGCCAGCACCGCAGGTGTAGGCCCCGGAGTCCCACCCGGCCTGCCCGCAAACAGCACCCCTTCC CAGGAAGAGAGGCGAGCCACCCAGTGGGGGTCCTTCTACAGCCCAGCCCAGACGGCCAACAATCCCTGC AGTAGATACGAAGACTATGGAATGACTCCCCCGACGGGTCCATTGCCAAGACCAGGTTTTGGCCCCGGT TTGCTGCAGTCTACAGAGCTGGTGCCCCCTGACCCTCAGCAGCCACAGGCCTCCGCCGAAGCCCCATTT GCTGCCAGAGGGATCTACTCGGAGGAGATGCCGTCGGTGGCCCGGCCTCGGCCTGTCGGGGGTACCACA GGCTCCCAGATCCAGCACCTGACACAGGTGGGGATTGCCAGCAGAATTGGAGCTCAGCCAGTGGAAATC CCGCCAAGCAGAGGCAGCCAGTATGGGGGGCCAGGCTGGCCTTCGTACGGGGAGGACGAAGCGGGGCGA AGAGAGGCCACACACATGCTCGGACATCAAGAGTATTCTTCTTCACCGCTATTTCAGGTGCCAAGGACT TCAGGCAGGGAGCCCTCAGCTCCTTCCGGGAACCTCCCCCACCGGGGACTGCAGGGCCCTGGGCTGGGT TACCCCACCAGCTCCACGGAAGACCTCCAGCCTGGCCACTCCTCGGCCTCTCTCATCAAAGCAATCCGC GAGGAGCTCCTCCGGCTCTCCCAGAAACAGAGCACCGTGCAGAACTTCCACAGCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001164665
Insert Size	5853 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001164665.1
RefSeq Size	12427 bp
RefSeq ORF	5853 bp
Locus ID	57670
UniProt ID	Q9HCM3
Protein Families	Transmembrane
MW	210.8 kDa
Gene Summary	The protein encoded by this gene belongs to the UPF0606 family. This gene has been found to be fused to the BRAF oncogene in many cases of pilocytic astrocytoma. The fusion results from 2Mb tandem duplications at 7q34. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012] Transcript Variant: This variant (2), also known as the long-form, v2, represents the longer transcript and encodes the longer isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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