OTOA (NM_001161683) Human Untagged Clone
CAT＃: SC327270
OTOA (untagged)-Human otoancorin (OTOA) transcript variant 3
CNY 20430.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CT108; DFNB22 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC327270 representing NM_001161683.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGAAACTCAATCACCTACAGGGACCAGGCAGCCGTGGAAAACCACCTGGAGCAGCGTCTGCACCAG CCCCAGAAGCTGCTGGAGGACCTGAGGAAGACAGACGCCCAGCAGTTCCGCACTGCCATGAAATGCCTC TTAGAAGACAAGAAGGACGGCTTGGACCTGAAAGACATCATCATCGACTTAGGAGAGATTCGAGAACGA GCCTTGCAGAGCCCTGGCGTGAACCGCAGCCTGTTTCTCATCACACTGGAGAGGTGTTTCCAGATGCTG AACTCCCTGGAGTGTGTGGAGATCCTGGGCAAGGTGCTGAGGGGGTCCTCAGGGAGCTTTCTCCAGCCA GACATCACAGAGCGGCTCCCTCGGGACCTGCGCGAGGATGCCTTTAAGAACCTATCTGCAGTGTTCAAA GATCTCTACGACAAAACCTCGGCTCATTCCCAGAGAGCTCTCTATTCCTGGATGACTGGAATACTGCAG ACATCCTCCAATGCCACTGATGACTCTGCTTCATGGGTCAGTGCGGAACACTTATGGGTTTTGGGCAGA TACATGGTTCACCTATCGTTTGAAGAAATTACGAAAATTAGTCCTATAGAAATTGGGCTGTTTATCAGC TATGACAACGCCACCAAGCAGCTGGACATGGTCTATGACATCACACCTGAGCTGGCCCAGGCGTTTCTG GAGAGGATCAGCTCCTCCAACTTTAACATGAGGAATACCTCCACCATCCACAGGCTGGGGCTGCTGGTT TGTTTCTACAATGACCTGGAATTGCTGGATGCCACTGTGGCTCAAGTCCTGCTTTACCAGATGATCAAG TGCAGCCACCTGAGGGGCTTCCAGGCTGGCGTCCAGAAGCTCAAAGCAGAACTCCTGGACATTGCCATG GAGAACCAGACCCTCAATGAGACCCTGGGTTCTTTGTCGGATGCAGTTGTAGGTTTGACCTACAGCCAA CTGGAATCCCTCTCCCCCGAGGCTGTGCACGGAGCCATCTCCACCCTCAACCAGGTCTCAGGTTGGGCC AAGAGCCAGGTCATCATCTTGTCTGCCAAATACTTGGCCCATGAGAAGGTGCTGTCTTTCTACAATGTC AGCCAGATGGGCGCACTGCTGGCTGGGGTCAGCACCCAGGCCTTCTGCAGCATGAAACGCAAGGACATC TCGCAGGTCCTGAGAAGTGCCGTCTCCCAGTATGTATCCGACTTGTCACCTGCCCAGCAGCAAGGTATC CTCAGCAAGATGGTCCAAGCGGAAGACACTGCCCCAGGCATCGTGGAGATACAAGGGGCTTTCTTTAAG GAAGTGTCTCTCTTTGATTTAAGGAGGCAACCTGGATTCAACTCTACAGTCCTGAAGGATAAGGAACTT GGAAGGAGCCAGGCTCTGTTCCTGTATGAGCTTCTGTTAAAGACCACCAGAAGGCCTGAGGAGCTTTTG AGTGCTGGGCAGCTGGTCAAAGGCGTGACCTGCTCACACATTGATGCCATGAGCACTGACTTCTTTCTG GCCCATTTCCAGGATTTTCAGAACAACTTCGCCCTGCTTTCACCCTATCAGGTTAATTGTTTGGCGTGG AAATACTGGGAAGTTTCCAGATTGTCTATGCCACCTTTCCTCTTGGCTGCACTCCCGGCCCGCTACCTG GCTTCTGTCCCAGCCTCCCAGTGTGTGCCCTTTCTGATCAGCCTGGGGAAGAGCTGGTTGGACTCCTTG GTTTTAGATTCCCACAAAAAGACTTCAGTCCTCAGGAAAGTGCAGCAGTGCCTGGACGACTCCATTGCT GATGAGTACACTGTGGACATCATGGGGAACCTGCTGTGTCACTTGCCGGCAGCCATCATCGACAGGGGG ATCTCCCCCAGGGCTTGGGCGACTGCTCTACACGGCCTCAGAGACTGCCCAGACCTCAACCCTGAGCAA AAGGCTGCAGTGAGGCTCAAGCTCCTGGGACAGTATGGACTCCCTCAGCACTGGACAGCCGAGACCACG AAGGACTTGGGACCCTTTCTAGTACTTTTCTCAGGAGATGAATTAAGCTCTATAGCCACAAAGTTTCCT GAGATCCTTCTGCAAGCAGCTTCCAAGATGGCCAGGACCCTGCCCACTAAAGAATTCCTCTGGGCTGTC TTTCAGTCTGTTCGGAACAGCAGTGATAAGATCCCCAGCTATGACCCTATGCCTGGTTGCCATGGAGTC GTGGCCCCCTCTTCTGATGACATCTTCAAGTTGGCCGAAGCCAACGCCTGCTGGGCCCTGGAGGACCTG CGGTGCATGGAGGAAGACACATTCATCAGGACCGTGGAACTGCTGGGAGCTGTCCAGGGTTTCAGCCGG CCTCAGCTGATGACCCTGAAGGAGAAAGCAATACAGGTTTGGGACATGCCATCTTACTGGAGAGAACAC CATATCGTCTCCCTGGGGCGCATTGCTCTGGCTCTTAATGAGAGTGAGCTGGAGCAGCTGGACCTCAGC TCCATAGACACTGTGGCTTCCCTAAGCTGGCAAACAGAATGGACCCCGGGACAGGCTGAATCCATTTTG CAAGGGTACCTGGATGATTCAGGATACAGTATCCAGGACCTGAAGAGCTTTCATTTGGTAGGACTTGGT GCAACCCTGTGTGCTATAAACATCACTGAAATCCCACTTATAAAGATCTCAGAATTCAGGGTGGTAGTG GCCAGAATTGGGACCCTGCTCTGCAGCACACATGTCTTAGCCGAGTTTAAGAGGAAGGCTGAAGTTGTG TTTGGGGATCCCACTGAGTGGACCAGTTCTGTCTTGCAGGAGCTTGGGACCATTGCAGCTGGATTAACT AAGGCAGAGCTCCGGATGCTTGACAAGGATTTGATGCCATATTTCCAGCCATCAGCAATAAAATGCCTT CCTGATGAGATATTCAAAGAGCTGTCCGCGGAGCAGATCGCCTCCCTGGGTCCGGAGAACGCCGCGGCG GTGACCCACGCCCAGCGCCGGCGGCTCAGTCCACTGCAGCTGCAGAGCCTCCAGCAGGCGCTAGATGGC GCCAAGACTCACTCCTGGCAGGACGCGCCCGCTAGCGCCGGTCCCACTAGAACCTCATCCTCGCGTTCT CCCGCAGGAGCTCTCCAGTCGTGGGGTCTTTGGCTTGGTTGTCCCCTGCTGGTTCTAATGGCCAAGCTC CTGTGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001161683 |
| Insert Size | 3183 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001161683.1 |
| RefSeq Size | 3480 bp |
| RefSeq ORF | 3183 bp |
| Locus ID | 146183 |
| UniProt ID | Q7RTW8 |
| MW | 117.9 kDa |
| Gene Summary | The protein encoded by this gene is specifically expressed in the inner ear, and is located at the interface between the apical surface of the inner ear sensory epithelia and their overlying acellular gels. It is prposed that this protein is involved in the attachment of the inner ear acellular gels to the apical surface of the underlying nonsensory cells. Mutations in this gene are associated with autosomal recessive deafness type 22 (DFNB22). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] Transcript Variant: This variant (3) is missing several coding exons, and contains an alternative exon at its 5' end compared to variant 1. This results in translation initiation from a different start codon, and a shorter isoform (3) with a distinct N-terminus compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228635 | OTOA (Myc-DDK-tagged)-Human otoancorin (OTOA), transcript variant 3 |
CNY 7384.00 |
|
| RC228635L3 | Lenti ORF clone of Human otoancorin (OTOA), transcript variant 3, Myc-DDK-tagged |
CNY 12450.00 |
|
| RC228635L4 | Lenti ORF clone of Human otoancorin (OTOA), transcript variant 3, mGFP tagged |
CNY 12450.00 |
|
| RG228635 | OTOA (tGFP-tagged) - Human otoancorin (OTOA), transcript variant 3 |
CNY 11590.00 |
