PDE2A (NM_001146209) Human Untagged Clone
CAT＃: SC327253
PDE2A (untagged)-Human phosphodiesterase 2A cGMP-stimulated (PDE2A) transcript variant 4
CNY 18050.00
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CGS-PDE; cGSPDE; PDE2A1; PED2A4 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001146209, the custom clone sequence may differ by one or more nucleotides
ATGAAGAAACAGAGGATCCAGGAGGGGAAATCGCTTGCCCACAGGCGGGGCCAGCAGGTC TTCCTCAAGCCGGACGAGCCGCCGCCGCCGCCGCAGCCATGCGCCGACAGCCTGCAGGAC GCCTTGCTGAGTCTGGGCTCTGTCATCGACATTTCAGGCCTGCAACGTGCTGTCAAGGAG GCCCTGTCAGCTGTGCTCCCCCGAGTGGAAACTGTCTACACCTACCTACTGGATGGTGAG TCCCAGCTGGTGTGTGAGGACCCCCCACATGAGCTGCCCCAGGAGGGGAAAGTCCGGGAG GCTATCATCTCCCAGAAGCGGCTGGGCTGCAATGGGCTGGGCTTCTCAGACCTGCCAGGG AAGCCCTTGGCCAGGCTGGTGGCTCCACTGGCTCCTGATACCCAAGTGCTGGTCATGCCG CTAGCGGACAAGGAGGCTGGGGCCGTGGCAGCTGTCATCTTGGTGCACTGTGGCCAGCTG AGTGATAATGAGGAATGGAGCCTGCAGGCGGTGGAGAAGCATACCCTGGTCGCCCTGCGG AGGGTGCAGGTCCTGCAGCAGCGCGGGCCCAGGGAGGCTCCCCGAGCCGTCCAGAACCCC CCGGAGGGGACGGCGGAAGACCAGAAGGGCGGGGCGGCGTACACCGACCGCGACCGCAAG ATCCTCCAACTGTGCGGGGAACTCTACGACCTGGATGCCTCTTCCCTGCAGCTCAAAGTG CTCCAATACCTGCAGCAGGAGACCCGGGCATCCCGCTGCTGCCTCCTGCTGGTGTCGGAG GACAATCTCCAGCTTTCTTGCAAGGTCATCGGAGACAAAGTGCTCGGGGAAGAGGTCAGC TTTCCCTTGACAGGATGCCTGGGCCAGGTGGTGGAAGACAAGAAGTCCATCCAGCTGAAG GACCTCACCTCCGAGGATGTACAACAGCTGCAGAGCATGTTGGGCTGTGAGCTGCAGGCC ATGCTCTGTGTCCCTGTCATCAGCCGGGCCACTGACCAGGTGGTGGCCTTGGCCTGCGCC TTCAACAAGCTAGAAGGAGACTTGTTCACCGACGAGGACGAGCATGTGATCCAGCACTGC TTCCACTACACCAGCACCGTGCTCACCAGCACCCTGGCCTTCCAGAAGGAACAGAAACTC AAGTGTGAGTGCCAGGCTCTTCTCCAAGTGGCAAAGAACCTCTTCACCCACCTGGATGAC GTCTCTGTCCTGCTCCAGGAGATCATCACGGAGGCCAGAAACCTCAGCAACGCAGAGATC TGCTCTGTGTTCCTGCTGGATCAGAATGAGCTGGTGGCCAAGGTGTTCGACGGGGGCGTG GTGGATGATGAGAGCTATGAGATCCGCATCCCGGCCGATCAGGGCATCGCGGGACACGTG GCGACCACGGGCCAGATCCTGAACATCCCTGACGCATATGCCCATCCGCTTTTCTACCGC GGCGTGGACGACAGCACCGGCTTCCGCACGCGCAACATCCTCTGCTTCCCCATCAAGAAC GAGAACCAGGAGGTCATCGGTGTGGCCGAGCTGGTGAACAAGATCAATGGGCCATGGTTC AGCAAGTTCGACGAGGACCTGGCGACGGCCTTCTCCATCTACTGCGGCATCAGCATCGCC CATTCTCTCCTATACAAAAAAGTGAATGAGGCTCAGTATCGCAGCCACCTGGCCAATGAG ATGATGATGTACCACATGAAGGTCTCCGATGATGAGTATACCAAACTTCTCCATGATGGG ATCCAGCCTGTGGCTGCCATTGACTCCAATTTTGCAAGTTTCACCTATACCCCTCGTTCC CTGCCCGAGGATGACACGTCCATGGCCATCCTGAGCATGCTGCAGGACATGAATTTCATC AACAACTACAAAATTGACTGCCCGACCCTGGCCCGGTTCTGTTTGATGGTGAAGAAGGGC TACCGGGATCCCCCCTACCACAACTGGATGCACGCCTTTTCTGTCTCCCACTTCTGCTAC CTGCTCTACAAGAACCTGGAGCTCACCAACTACCTCGAGGACATCGAGATCTTTGCCTTG TTTATTTCCTGCATGTGTCATGACCTGGACCACAGAGGCACAAACAACTCTTTCCAGGTG GCCTCGAAATCTGTGCTGGCTGCGCTCTACAGCTCTGAGGGCTCCGTCATGGAGAGGCAC CACTTTGCTCAGGCCATCGCCATCCTCAACACCCACGGCTGCAACATCTTTGATCATTTC TCCCGGAAGGACTATCAGCGCATGCTGGATCTGATGCGGGACATCATCTTGGCCACAGAC CTGGCCCACCATCTCCGCATCTTCAAGGACCTCCAGAAGATGGCTGAGGTGGGCTACGAC CGAAACAACAAGCAGCACCACAGACTTCTCCTCTGCCTCCTCATGACCTCCTGTGACCTC TCTGACCAGACCAAGGGCTGGAAGACTACGAGAAAGATCGCGGAGCTGATCTACAAAGAA TTCTTCTCCCAGGGAGACCTGGAGAAGGCCATGGGCAACAGGCCGATGGAGATGATGGAC CGGGAGAAGGCCTATATCCCTGAGCTGCAAATCAGCTTCATGGAGCACATTGCAATGCCC ATCTACAAGCTGTTGCAGGACCTGTTCCCCAAAGCGGCAGAGCTGTACGAGCGCGTGGCC TCCAACCGTGAGCACTGGACCAAGGTGTCCCACAAGTTCACCATCCGCGGCCTCCCAAGT AACAACTCGCTGGACTTCCTGGATGAGGAGTACGAGGTGCCTGATCTGGATGGCACTAGG GCCCCCATCAATGGCTGCTGCAGCCTTGATGCTGAG |
| Restriction Sites | Please inquire |
| ACCN | NM_001146209 |
| Insert Size | 4196 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001146209.1, NP_001139681.1 |
| RefSeq Size | 4196 bp |
| RefSeq ORF | 2799 bp |
| Locus ID | 5138 |
| UniProt ID | O00408 |
| Protein Families | Druggable Genome |
| Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
| Gene Summary | Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Plays an important role in growth and invasion of malignant melanoma cells (e.g. pseudomyxoma peritonei (PMP) cell line) (PubMed:24705027).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate start codon, compared to variant 1. The encoded isoform, PDE2A4 (AY495087.1), is shorter and has a distinct N-terminus, compared to isoform PDE2A3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228618 | PDE2A (Myc-DDK-tagged)-Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 4 |
CNY 7130.00 |
|
| RC228618L3 | Lenti-ORF clone of PDE2A (Myc-DDK-tagged)-Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 4 |
CNY 9030.00 |
|
| RC228618L4 | Lenti-ORF clone of PDE2A (mGFP-tagged)-Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 4 |
CNY 9030.00 |
|
| RG228618 | PDE2A (tGFP-tagged) - Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 4 |
CNY 7890.00 |
