LOXHD1 (NM_001145473) Human Untagged Clone
CAT＃: SC326588
LOXHD1 (untagged)-Human lipoxygenase homology domains 1 (LOXHD1), transcript variant 3, mRNA
CNY 8270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DFNB77; LH2D1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC326588 representing NM_001145473.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACGGTGTGGACAGGGGATGTGGTTGGCGGGGGCACTGACTCCAACATCTTCATGACCCTCTACGGC ATCAACGGGAGCACAGAGGAGATGCAGCTGGACAAAAAGAAAGCCAGGTTTGAGCGGGAGCAGAACGAC ACCTTCATCATGGAGATCCTAGACATTGCTCCATTCACCAAGATGCGGATCCGGATTGATGGCCTGGGC AGTCGGCCGGAGTGGTTCCTGGAGAGGATCCTACTGAAGAACATGAACACTGGAGACCTGACCATGTTC TACTATGGAGACTGGCTGTCCCAGCGGAAGGGCAAGAAGACCCTGGTGTGTGAAATGTGTGCCGTTATC GATGAGGAAGAAATGATGGAGTGGACCTCCTACACCGTCGCAGTTAAGACCAGCGACATCCTGGGAGCA GGCACTGATGCCAACGTGTTCATCATCATCTTCGGGGAGAACGGGGATAGTGGGACACTGGCCCTGAAG CAGTCGGCAAACTGGAACAAGTTTGAGCGGAACAACACGGACACATTCAACTTCCCTGACATGCTGAGC TTGGGCCACCTCTGCAAGCTGAGGGTCTGGCACGACAACAAAGGGATATTTCCTGGCTGGCATCTGAGC TATGTCGATGTGAAGGACAACTCCCGCGACGAGACCTTCCACTTCCAGTGTGACTGCTGGCTCTCCAAG AGTGAGGGTGACGGGCAGACGGTCCGCGACTTTGCCTGTGCCAACAACAAGATCTGTGATGAGCTGGAA GAGACCACCTACGAGATCGTCATAGAAACGGGCAACGGAGGCGAAACCAGGGAGAACGTCTGGCTCATC CTGGAGGGCAGGAAGAACCGATCCAAAGAGTTTCTCATGGAAAATTCTTCTAGGCAGCGGGCCTTTAGG AAGGGGACCACAGACACGTTTGAGTTTGACAGCATCTACTTGGGGGACATTGCCTCCCTCTGTGTGGGC CACCTTGCCAGGGAAGACCGGTTTATCCCCAAGAGAGAACTTGCCTGGCATGTCAAGACCATCACCATC ACCGAGATGGAGTACGGCAATGTGTACTTCTTTAACTGTGACTGCCTCATCCCCCTCAAGAGGAAGAGG AAGTACTTCAAGGTATTCGAGGTTACCAAGACGACAGAGAGCTTTGCCAGCAAGGTCCAGAGCCTGGTG CCCGTCAAGTACGAAGTCATCGTGACAACAGGCTATGAGCCAGGGGCAGGCACTGATGCCAACGTCTTC GTGACCATCTTTGGGGCCAACGGAGACACAGGCAAGCGGGAGCTGAAGCAGAAAATGCGCAACCTCTTC GAGCGGGGCAGCACAGACCGCTTCTTCCTGGAGACGCTGGAGCTGGGTGAGCTGCGCAAGGTGCGCCTG GAGCACGACAGCAGTGGCTACTGCTCAGGCTGGCTGGTGGAGAAGGTGGAGGTCACCAACACCAGCACC GGCGTGGCCACCATCTTCAACTGTGGCAGGTGGCTGGACAAGAAGCGGGGGGATGGACTCACCTGGAGA GACCTCTTCCCTTCTGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001145473 |
| Insert Size | 1539 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001145473.2 |
| RefSeq Size | 1977 bp |
| RefSeq ORF | 1539 bp |
| Locus ID | 125336 |
| UniProt ID | Q8IVV2 |
| MW | 58.7 kDa |
| Gene Summary | This gene encodes a highly conserved protein consisting entirely of PLAT (polycystin/lipoxygenase/alpha-toxin) domains, thought to be involved in targeting proteins to the plasma membrane. Studies in mice show that this gene is expressed in the mechanosensory hair cells in the inner ear, and mutations in this gene lead to auditory defects, indicating that this gene is essential for normal hair cell function. Screening of human families segregating deafness identified a mutation in this gene which causes DFNB77, a progressive form of autosomal-recessive nonsyndromic hearing loss (ARNSHL). Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2010] Transcript Variant: This variant (3) is missing many exons from the 5' end, and contains an alternate 5' terminal exon compared to variant 1. This results in translation initiation from an in-frame downstream AUG, and a shorter isoform (3) compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227228 | LOXHD1 (Myc-DDK-tagged)-Human lipoxygenase homology domains 1 (LOXHD1), transcript variant 3 |
CNY 3816.00 |
|
| RC227228L3 | Lenti ORF clone of Human lipoxygenase homology domains 1 (LOXHD1), transcript variant 3, Myc-DDK-tagged |
CNY 6370.00 |
|
| RC227228L4 | Lenti ORF clone of Human lipoxygenase homology domains 1 (LOXHD1), transcript variant 3, mGFP tagged |
CNY 6370.00 |
|
| RG227228 | LOXHD1 (tGFP-tagged) - Human lipoxygenase homology domains 1 (LOXHD1), transcript variant 3 |
CNY 4940.00 |
