PNPLA1 (NM_001145717) Human Untagged Clone
CAT＃: SC326582
PNPLA1 (untagged)-Human patatin-like phospholipase domain containing 1 (PNPLA1), transcript variant 3, mRNA
CNY 8550.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARCI10; dJ50J22.1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC326582 representing NM_001145717.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAAGAACAGGTGTTCAAGGGGGACCCGGACACCCCTCACTCCATCTCCTTCTCGGGCAGTGGATTC CTCTCCTTCTACCAGGCGGGGGCTGTGGACGCCCTGCGGGACCTGGCCCCCCGGATGCTGGAAACAGCC CACCGCTTTGCGGGGACATCGGCAGGTGCTGTGATCGCCGCCCTGGCCATCTGCGGGATTGAAATGGAT GAGTATCTCAGAGTCCTCAACGTGGGTGTGGCCGAGGTGAAGAAATCCTTCCTGGGGCCCTTGTCCCCG TCCTGTAAGATGGTGCAGATGATGAGGCAGTTTCTGTACCGGGTCCTGCCCGAGGACTCCTACAAGGTC ACCACGGGGAAGCTCCATGTGAGCCTCACCCGCTTAACGGACGGGGAGAATGTGGTGGTTTCAGAGTTC ACGTCCAAGGAGGAGCTCATTGAGGCCCTATACTGCAGCTGCTTCGTCCCGGTGTACTGTGGCCTCATC CCCCCGACTTACCGCGGTGTGAGGTACATCGATGGGGGCTTCACGGGCATGCAGCCCTGTGCCTTCTGG ACCGACGCCATCACCATCTCCACCTTCAGTGGGCAGCAGGACATCTGTCCCCGGGACTGCCCGGCCATC TTCCACGACTTCCGCATGTTCAACTGCTCCTTCCAGTTCTCCCTGGAGAACATCGCCAGGATGACCCAC GCATTGTTCCCCCCGGACCTGGTGATCCTGCACGATTACTACTACCGAGGGTACGAGGATGCAGTTTTG TACTTGAGGCGGCTGAATGCTGTTTATCTTAATTCTTCCTCCAAGAGAGTGATTTTCCCCCGGGTGGAA GTGTACTGCCAGATAGAACTCGCCCTTGGCAATGAGTGCCCTGAACGCAGTCAACCAAGCCTTCGAGCA CGGCAGGCCAGTCTGGAAGGAGCCACACAACCTCACAAGGAGTGGGTTCCCAAAGGGGATGGAAGGGGC AGCCATGGTCCGCCTGTGTCCCAACCTGTGCAGACACTTGAATTCACATGCGAGTCACCTGTTTCAGCA CCAGTCTCTCCACTTGAGCAGCCACCTGCACAGCCACTGGCCTCTTCAACTCCACTTTCTCTAAGTGGC ATGCCACCTGTATCATTCCCAGCTGTGCACAAGCCACCCAGCTCCACACCTGGTTCATCACTGCCCACC CCACCACCTGGACTGTCACCTCTGTCACCTCAGCAGCAGGTACAACCGTCTGGATCACCAGCCAGATCC CTACACTCTCAGGCACCCACTTCACCCAGGCCATCCCTGGGGCCTTCAACTGTGGGGGCACCTCAAACA CTGCCCCGAAGTTCTCTTTCAGCCTTCCCTGCTCAGCCACCTGTGGAGGAACTAGGCCAAGAACAGCCC CAAGCTGTAGCTCTTCTTGTCTCTTCAAAACCAAAAAGCGCCGTGCCTCTGGTTCATGTGAAGGAAACC GTCAGCAAGCCTTATGTAACGGAGAGCCCTGCTGAAGACTCAAACTGGGTGAATAAGGTCTTCAAGAAG AACAAGCAAAAGACAAGTGGCACCAGAAAAGGCTTCCCAAGACATTCGGGATCCAAAAAACCAAGCAGC AAAGTGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001145717 |
| Insert Size | 1599 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001145717.1 |
| RefSeq Size | 2478 bp |
| RefSeq ORF | 1599 bp |
| Locus ID | 285848 |
| UniProt ID | Q8N8W4 |
| MW | 57.9 kDa |
| Gene Summary | The protein encoded by this gene belongs to the patatin-like phospholipase (PNPLA) family, which is characterized by the presence of a highly conserved patatin domain. PNPLA family members have diverse lipolytic and acyltransferase activities, and are key elements in lipid metabolism. While other members of this family have been well characterized, the function of this gene remained an enigma. However, recent studies show that this gene is expressed in the skin epidermal keratinocytes, and has a role in glycerophospholipid metabolism in the cutaneous barrier. Consistent with these observations, mutations in this gene are associated with ichthyosis in human (autosomal recessive congenital ichthyoses, ARCI) and dog. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012] Transcript Variant: This variant (3) contains an alternate 5' terminal exon compared to variant 1, resulting in translation initiation from an in-frame upstream start codon, and a longer isoform (3) with a distinct N-terminus compared to isoform 1. Isoform 3 contains the N-terminal catalytic patatin domain, that is conserved in other mammalian orthologs (mouse, rat, cow), and essential for lipase activity (PMIDS:16799181, 22246504). |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227055 | PNPLA1 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 1 (PNPLA1), transcript variant 3 |
CNY 3960.00 |
|
| RC227055L3 | Lenti ORF clone of Human patatin-like phospholipase domain containing 1 (PNPLA1), transcript variant 3, Myc-DDK-tagged |
CNY 6460.00 |
|
| RC227055L4 | Lenti ORF clone of Human patatin-like phospholipase domain containing 1 (PNPLA1), transcript variant 3, mGFP tagged |
CNY 6460.00 |
|
| RG227055 | PNPLA1 (tGFP-tagged) - Human patatin-like phospholipase domain containing 1 (PNPLA1), transcript variant 3 |
CNY 5040.00 |
