TSEN2 (NM_001145394) Human Untagged Clone
CAT＃: SC326571
TSEN2 (untagged)-Human tRNA splicing endonuclease 2 homolog (S. cerevisiae) (TSEN2), transcript variant 4, mRNA
CNY 6560.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PCH2B; SEN2; SEN2L |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC326571 representing NM_001145394.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAGAAGCAGTTTTCCATGCCCCAAAGAGGAAAAGAAGAGTGTATGAGACTTACGAGTCTCCATTG CCAATCCCTTTTGGTCAGGACCATGGTCCTCTGAAAGAATTCAAGATATTCCGTGCTGAAATGATTAAC AACAATGTGATTGTGAGGAATGCGGAGGACATTGAGCAGCTCTATGGGAAAGGTTATTTTGGAAAAGGT ATTCTTTCAAGAAGCCGTCCAAGCTTCACAATTTCAGATCCTAAACTGGTTGCTAAATGGAAAGATATG AAGACAAACATGCCTATCATCACATCAAAGAGGTATCAGCATAGTGTTGAGTGGGCAGCAGAGCTGATG CGTAGACAGGGGCAGGATGAGAGTACAGTGCGCAGAATCCTCAAGGATTACACGAAACCGCTTGAGCAT CCTCCTGTGAAAAGGAATGAAGAGGCTCAAGTGCATGACAAGCTTAACTCTGGAATGGTTTCCAACATG GAAGGCACAGCAGGGGGAGAGAGACCTTCTGTGCGTGGCCTTCATCATGAAGACGGCAGCCAGCACATC GGCCTCCTGCATCCTGGGGACAGAGGGCCTGACCATGAGTACGTGCTGGTCGAGGAAGCGGAGTGTGCC ATGAGCGAGAGGGAGGCTGCCCCAAATGAGGAATTGGTGCAAAGAAACAGGTTAATATGCAGAAGAAAT CCATATAGGATCTTTGAGTATTTGCAACTCAGCCTAGAAGAGGCCTTTTTCTTGGTCTATGCTCTGGGA TGTTTAAGTATTTACTATGAGAAGGAGCCTTTAACGATAGTGAAGCTCTGGAAAGCTTTCACTGTAGTT CAGCCCACGTTCAGAACCACCTACATGGCCTACCATTACTTTCGAAGCAAGGGCTGGGTGCCCAAAGTG GGACTCAAGTACGGGACAGATTTACTGCTATATCGGAAAGGCCCTCCATTTTACCATGCAAGTTATTCT GTCATTATCGAGCTAGTTGATGACCATTTTGAAGGCTCTCTCCGCAGGCCTCTCAGTTGGAAGTCCCTG GCTGCCTTGAGCAGAGTTTCCGTTAATGTCTCTAAGGAACTTATGCTGTGCTATTTGATTAAACCCTCT ACTATGACTGACAAGGAAATGGAGTCACCAGAATGTATGAAAAGGATTAAAGTTCAGGAGGTGATTCTG AGTCGATGGGTTTCTTCACGAGAGAGGAGTGACCAAGACGATCTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001145394 |
| Insert Size | 1221 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001145394.1 |
| RefSeq Size | 1941 bp |
| RefSeq ORF | 1221 bp |
| Locus ID | 80746 |
| UniProt ID | Q8NCE0 |
| MW | 47.1 kDa |
| Gene Summary | This gene encodes one of the subunits of the tRNA splicing endonuclease. This endonuclease catalyzes the first step in RNA splicing which is the removal of introns. Mutations in this gene have been associated with pontocerebellar hypoplasia type 2. A pseudogene has been identified on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009] Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate in-frame splice site in the coding region compared to variant 1. The resulting protein (isoform 3) is shorter than isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227271 | TSEN2 (Myc-DDK-tagged)-Human tRNA splicing endonuclease 2 homolog (S. cerevisiae) (TSEN2), transcript variant 4 |
CNY 3656.00 |
|
| RC227271L3 | Lenti-ORF clone of TSEN2 (Myc-DDK-tagged)-Human tRNA splicing endonuclease 2 homolog (S. cerevisiae) (TSEN2), transcript variant 4 |
CNY 5890.00 |
|
| RC227271L4 | Lenti-ORF clone of TSEN2 (mGFP-tagged)-Human tRNA splicing endonuclease 2 homolog (S. cerevisiae) (TSEN2), transcript variant 4 |
CNY 5890.00 |
|
| RG227271 | TSEN2 (tGFP-tagged) - Human tRNA splicing endonuclease 2 homolog (S. cerevisiae) (TSEN2), transcript variant 4 |
CNY 4370.00 |
