MyoGEF (PLEKHG6) (NM_001144857) Human Untagged Clone
CAT＃: SC326224
PLEKHG6 (untagged)-Human pleckstrin homology domain containing, family G (with RhoGef domain) member 6 (PLEKHG6), transcript variant 3
CNY 8336.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MyoGEF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC326224 representing NM_001144857
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGATGCAGGCTGCACGCCCCTGGGGAGAAGGCTGCACATGATCCCAGTCGCCGACGCCTCCAGCAGT ATGTCCCCTTTGCCAGGGGTTCTGGCCAGGCCCGAGGCCTGTCACCCATGAGACTGCGAGATCCAGAGCC CGAGAAGAGGCACGGGGGCCATGTGGGGGCTGGCCTGCTTCACTCCCCCAAACTCAAGGAACTCACCAAG GCCCATGAGCTGGAGGTGAGGCTGCACACTTTCAGCATGTTTGGGATGCCCCGGCTGCCCCCTGAGGACC GGCGGCACTGGGAGATAGGAGAGGGTGGCGACAGTGGCCTGACCATCGAGAAGTCCTGGAGGGAGCTGGT GCCTGGGCACAAGGAGATGAGCCAGGAGCTCTGCCACCAACAGGAGGCCCTGTGGGAGCTCCTGACCACC GAGCTGATCTACGTGAGAAAGCTCAAGATCATGACTGATCTGCTAGCCGCCGGCCTGCTGAACCTGCAGC GAGTGGGACTGCTGATGGAAGTGTCAGCTGAGACCCTGTTTGGAAATGTCCCCAGCCTGATTCGAACCCA CCGGAGCTTTTGGGATGAGGTGCTGGGGCCCACCCTGGAGGAGACTCGGGCCTCGGGCCAGCCTCTGGAC CCCATTGGTCTGCAAAGTGGCTTCCTGACGTTTGGCCAGCGGTTCCACCCCTATGTCCAGTACTGCCTCC GAGTGAAGCAGACCATGGCTTACGCCCGAGAACAGCAAGAAACTAACCCTCTCTTCCATGCCTTCGTGCA GTGGTGTGAGAAGCACAAGCGCTCTGGGAGGCAGATGCTCTGTGACTTGCTTATCAAGCCCCACCAGCGC ATCACCAAGTACCCACTGCTGCTCCATGCTGTGCTCAAGAGGAGCCCCGAGGCACGAGCCCAAGAGGCCC TGAATGCCATGATTGAAGCCGTGGAGTCATTCCTGCGACACATCAATGGGCAGGTCCGCCAGGGCGAAGA GCAAGAGAGCTTGGCGGCTGCAGCACAACGCATCGGGCCCTACGAGGTGCTGGAGCCACCCAGTGATGAG GTGGAGAAGAACCTGCGCCCATTCTCCACCCTGGACCTGACGTCCCCCATGCTGGGGGTTGCATCTGAGC ACACCAGACAGCTGCTGCTGGAGGGGCCTGTGCGAGTGAAGGAGGGACGAGAAGGGAAGCTGGACGTGTA CCTGTTCCTCTTCTCTGATGTGCTCCTTGTGACCAAGCCCCAGCGCAAGGCGGACAAAGCCAAGGTCATC CGACCCCCTCTCATGCTGGAGAAGCTCGTGTGCCAACCCCTGCGAGACCCCAACAGCTTCCTGCTGATCC ACCTCACTGAATTCCAGTGTGTCTCCAGCGCCCTCCTTGTGCACTGTCCCAGTCCTACAGACCGTGCCCA GTGGCTGGAGAAGACCCAGCAGGCCCAGGCCGCCCTACAGAAGCTGAAGGCAGAGGAGTATGTTCAACAG AAGAGGGAGCTCCTGACCCTCTATCGGGACCAGGACAGGGAGTCCCCCAGCACCAGGCCCTCCACGCCTT CCCTGGAGGGCTCTCAGAGCAGCGCAGAGGGGAGGACTCCTGAGTTCTCGACCATTATCCCCCACCTGGT GGTGACAGAAGACACAGATGAAGATGCTCCCCTTGTGCCAGATGATACCTCAGACTCTGGCTACGGCACT TTGATCCCAGGCACCCCCACGGGGTCCCGCTCCCCACTGAGCCGTCTACGCCAAAGAGCCCTTCGGCGGG ACCCTCGCCTCACCTTCTCCACCCTGGAACTCCGGGACATCCCTCTGCGTCCCCACCCTCCCGACCCCCA AGCTCCTCAACGCCGAAGCGCCCCCGAACTGCCGGAAGGAATCCTAAAAGGAGGCAGTCTTCCCCAGGAA GACCCACCAACCTGGTCTGAGGAAGAAGATGGGGCCTCCGAGCGAGGGAATGTGGTGGTGGAAACACTCC ACAGGGCCCGGCTTCGGGGCCAGCTTCCCTCCTCCCCAACCCATGCTGACTCTGCCGGGGAAAGCCCCTG GGAGTCCTCAGGGGAGGAGGAAGAAGAGGGGCCTCTGTTCCTGAAAGCTGGCCACACATCCCTGCGCCCA ATGCGGGCTGAGGACATGCTCAGAGAGATCCGGGAGGAGCTGGCCAGCCAAAGGATTGAGGGGGCCGAGG AGCCCCGGGACAGCAGGCCACGGAAGCTGACTCGGGCCCAGCTGCAGAGGATGCGGGGGCCCCACATCAT TCAGCTGGACACCCCTCTGTCCGCATCAGAGGTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001144857 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001144857.1, NP_001138329.1 |
| RefSeq Size | 3149 bp |
| RefSeq ORF | 2277 bp |
| Locus ID | 55200 |
| UniProt ID | Q3KR16 |
| Gene Summary | Guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation. Also activates RHOG. Does not activate RAC1, or to a much lower extent than RHOA and RHOG. Part of a functional unit, involving PLEKHG6, MYH10 and RHOA, at the cleavage furrow to advance furrow ingression during cytokinesis. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with EZR, required for normal macropinocytosis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting protein (isoform b) has a shorter and distinct N-terminus, compared to isoform a. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227365 | PLEKHG6 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family G (with RhoGef domain) member 6 (PLEKHG6), transcript variant 3 |
CNY 8328.00 |
|
| RC227365L3 | Lenti ORF clone of Human pleckstrin homology domain containing, family G (with RhoGef domain) member 6 (PLEKHG6), transcript variant 3, Myc-DDK-tagged |
CNY 9980.00 |
|
| RC227365L4 | Lenti ORF clone of Human pleckstrin homology domain containing, family G (with RhoGef domain) member 6 (PLEKHG6), transcript variant 3, mGFP tagged |
CNY 9980.00 |
|
| RG227365 | PLEKHG6 (tGFP-tagged) - Human pleckstrin homology domain containing, family G (with RhoGef domain) member 6 (PLEKHG6), transcript variant 3 |
CNY 8930.00 |
