GRAMD1A (NM_001136199) Human Untagged Clone
CAT＃: SC326198
GRAMD1A (untagged)-Human GRAM domain containing 1A (GRAMD1A), transcript variant 2
CNY 13780.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KIAA1533 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC326198 representing NM_001136199.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTCGACACCACACCCCACTCTGGCCGGAGCACGCCAAGCAGCTCCCCATCGCTCCGGAAACGGCTG CAGCTCCTGCCCCCAAGCCGGCCCCCACCTGAGCCAGAACCAGGCACCATGGTGGAGAAGGGATCAGAT AGCTCCTCAGAGAAGGGTGGGGTGCCTGGGACCCCCAGCACCCAGAGCCTAGGCAGCCGGAACTTCATC CGCAACAGCAAGATGCTGAGCCCCACTTATAAGCAGCGTAATGAGGACTTCCGGAAACTGTTCAGCAAA CTCCCCGAAGCAGAACGCCTCATTGTGGATTACTCCTGCGCCCTGCAGCGTGAGATCCTGCTCCAGGGC CGCCTCTACCTCTCTGAGAACTGGATCTGCTTCTACAGCAACATCTTCCGCTGGGAGACCACGATCTCC ATCCAGCTGAAGGAAGTGACATGTCTGAAGAAGGAAAAGACGGCCAAGCTGATCCCCAACGCCATCCAG ATCTGCACGGAGAGCGAGAAGCATTTCTTCACTTCCTTTGGGGCCCGTGACCGCTGCTTCCTCCTCATC TTCCGCCTCTGGCAGAATGCACTGCTTGAAAAGACGCTGAGTCCCCGCGAGCTCTGGCACCTGGTGCAT CAGTGCTACGGCTCAGAGCTGGGCCTCACCAGTGAGGATGAGGACTATGTCTCCCCCTTGCAGCTGAAC GGTCTGGGGACCCCCAAGGAAGTGGGAGATGTGATCGCCCTGAGCGACATCACCTCCTCGGGGGCAGCT GACCGCAGCCAGGAGCCAAGCCCAGTGGGTTCGCGCCGTGGCCATGTCACGCCCAACCTTTCCCGAGCC AGCAGCGACGCAGACCATGGGGCAGAGGAGGACAAGGAGGAGCAGGTAGACAGCCAGCCAGACGCCTCC TCCAGCCAGACAGTGACCCCGGTGGCTGAACCCCCGAGCACAGAGCCCACCCAGCCTGACGGGCCCACC ACCCTGGGCCCCTTGGATCTGCTGCCCAGTGAGGAGCTATTGACAGACACAAGTAACTCCTCTTCATCC ACTGGGGAGGAAGCGGACTTGGCTGCCCTGCTTCCCGACCTCTCCGGCCGCCTCCTCATCAACTCTGTC TTCCATGTGGGCGCTGAGCGGCTCCAGCAGATGCTCTTCTCGGACTCGCCCTTCCTCCAGGGCTTCCTA CAGCAGTGCAAGTTCACAGACGTGACGCTGAGCCCCTGGAGTGGGGACAGCAAGTGCCACCAGCGCCGG GTGCTGACGTACACCATCCCCATCAGCAACCCACTGGGCCCCAAGAGCGCCTCCGTGGTGGAGACACAG ACGCTGTTCCGGCGCGGCCCCCAGGCCGGCGGGTGTGTGGTGGACTCCGAGGTGCTGACGCAGGGCATC CCCTACCAGGACTACTTCTACACTGCCCACCGCTACTGCATCCTGGGTCTGGCCCGGAACAAGGCGCGG CTCCGAGTGTCTTCTGAGATCCGCTACCGAAAGCAGCCGTGGAGCCTGGTGAAGTCGCTCATTGAGAAG AACTCGTGGAGCGGCATTGAAGACTATTTCCACCATCTGGAGCGAGAGCTCGCCAAGGCTGAGAAGCTG TCTCTGGAGGAAGGCGGGAAGGATGCCCGGGGCTTGCTATCCGGCCTGCGGCGGCGGAAGCGGCCCCTG AGCTGGCGGGCTCACGGGGACGGGCCCCAGCACCCAGATCCTGACCCCTGTGCCCGGGCCGGCATTCAC ACCTCGGGCTCCCTCAGCTCCCGCTTCTCCGAACCATCTGTGGACCAGGGCCCCGGGGCAGGCATCCCC AGTGCCCTGGTTCTCATCAGCATTGTCCTTATCATCCTCATCGCCCTCAACGTCCTGCTCTTCTACCGC CTCTGGTCCCTGGAAAGGACAGCCCACACCTTTGAGTCCTGGCACAGCCTGGCCCTGGCCAAGGGCAAG TTCCCCCAGACGGCCACAGAGTGGGCCGAGATCCTGGCGCTGCAGAAGCAATTCCACAGCGTGGAGGTG CACAAGTGGAGGCAGATCCTGCGGGCCTCCGTGGAGCTCCTGGATGAGATGAAGTTCTCGCTGGAGAAG CTGCACCAAGGCATCACAGTCTCAGACCCTCCCTTTGACACCCAGCCCCGGCCCGATGACAGCTTTTCC TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001136199 |
| Insert Size | 2142 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001136199.2 |
| RefSeq Size | 2649 bp |
| RefSeq ORF | 2142 bp |
| Locus ID | 57655 |
| UniProt ID | Q96CP6 |
| Protein Families | Transmembrane |
| MW | 79.4 kDa |
| Gene Summary | Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (By similarity). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (By similarity). Plays a crucial role in cholesterol homeostasis and has the unique ability to localize to the PM based on the level of membrane cholesterol (By similarity). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (By similarity). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (By similarity). May play a role in tumor progression (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks two alternate in-frame exons, compared to variant 1. The encoded isoform (2) has the same N- and C-termini, but is 11 amino acids shorter than isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226692 | GRAMD1A (Myc-DDK-tagged)-Human GRAM domain containing 1A (GRAMD1A), transcript variant 2 |
CNY 5224.00 |
|
| RC226692L3 | Lenti ORF clone of Human GRAM domain containing 1A (GRAMD1A), transcript variant 2, Myc-DDK-tagged |
CNY 9410.00 |
|
| RC226692L4 | Lenti ORF clone of Human GRAM domain containing 1A (GRAMD1A), transcript variant 2, mGFP tagged |
CNY 9410.00 |
|
| RG226692 | GRAMD1A (tGFP-tagged) - Human GRAM domain containing 1A (GRAMD1A), transcript variant 2 |
CNY 8270.00 |
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