FLASH (CASP8AP2) (NM_001137668) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CED-4; FLASH; RIP25
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC325424 representing NM_001137668. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAGCAGATGATGACAATGGTGATGGAACAAGTTTATTTGATGTCTTTTCTGCTTCTCCTCTTAAG AACAATGATGAAGGCTCACTGGACATATACGCTGGGTTGGACAGTGCTGTTTCTGACAGCGCTTCCAAA TCCTGTGTACCATCAAGAAATTGTTTGGACTTATATGAAGAGATCCTGACTGAAGAAGGAACTGCAAAG GAGGCAACATATAATGATTTGCAAGTAGAATATGGAAAATGTCAACTACAAATGAAAGAGCTGATGAAA AAATTTAAAGAAATACAGACACAGAATTTCAGCTTAATAAACGAAAACCAGTCTCTTAAGAAGAATATT TCAGCACTTATCAAAACTGCCAGAGTGGAAATAAACCGCAAGGATGAAGAAATAAGTAATCTTCACCAA AGATTGTCTGAGTTTCCACATTTTCGAAATAATCATAAAACTGCAAGGACATTTGATACAGTTAAAACA AAAGATCTTAAATCTAGATCTCCACATTTGGATGATTGTTCAAAGACTGATCACAGAGCTAAAAGTGAT GTTTCTAAAGATGTACATCATAGCACTTCACTGCCAAATCTGGAAAAGGAAGGAAAACCACATTCTGAT AAAAGGAGTACTTCACATTTACCTACATCTGTTGAGAAACACTGCACTAATGGTGTTTGGTCACGTTCT CATTATCAGGTTGGCGAGGGTAGCTCAAATGAGGATAGTAGAAGAGGAAGAAAAGATATTAGACATAGC CAGTTTAACAGAGGAACTGAAAGAGTACGAAAAGACTTAAGTACTGGCTGTGGTGATGGTGAACCAAGG ATATTGGAGGCTAGTCAAAGGCTACAAGGACATCCTGAGAAATATGGTAAAGGTGAACCAAAGACTGAA AGCAAAAGTTCGAAGTTTAAAAGTAACTCAGATTCTGACTATAAAGGTGAACGCATTAACTCTTCTTGG GAGAAAGAGACCCCTGGAGAAAGGTCACACAGTCGAGTAGACTCTCAAAGTGACAAAAAACTAGAAAGA CAAAGTGAAAGATCACAAAATATAAATAGGAAAGAAGTTAAATCACAAGACAAAGAAGAAAGAAAAGTT GATCAAAAACCTAAATCAGTAGTAAAGGACCAAGATCACTGGAGAAGATCTGAACGAGCATCACTTCCT CATTCCAAGAATGAAATAACATTTTCTCATAATTCAAGTAAATACCATCTAGAAGAGAGAAGAGGATGG GAAGATTGTAAAAGAGACAAGAGTGTAAACAGTCATAGTTTTCAAGATGGAAGATGTCCATCTTCTCTT TCAAACAGTAGAACTCACAAAAACATTGACTCTAAGGAAGTTGATGCCATGCATCAGTGGGAAAATACA CCTTTAAAAGCAGAAAGACATAGAACTGAAGATAAGAGGAAAAGAGAACAAGAAAGCAAAGAAGAAAAT AGGCATATTAGAAATGAAAAAAGAGTACCTACAGAACATTTGCAGAAGACTAATAAGGAAACTAAGAAA ACCACTACTGATTTAAAGAAACAGAATGAACCAAAGACTGATAAGGGAGAAGTCCTTGATAATGGTGTT TCTGAAGGAGCAGATAATAAAGAGCTTGCAATGAAAGCTGAGAGTGGTCCAAATGAAACAAAAAACAAG GACCTAAAATTGAGTTTTATGAAAAAATTGAACTTAACTCTTTCTCCTGCTAAAAAGCAACCTGTTTCC CAGGATAATCAGCATAAAATAACTGATATTCCCAAGTCCAGTGGTGTATGTGATTCAGAGTCTTCAATG CAAGTTAAAACAGTGGCATATGTTCCCTCCATAAGTGAACATATCTTGGGGGAAGCAGCTGTCAGTGAA CATACCATGGGGGAAACCAAGTCAACGTTATTGGAACCAAAGGTTGCTCTTCTAGCAGTGACTGAACCC AGGATCGGTATCTCAGAAACCAACAAGGAAGACGAAAATAGTTTGTTAGTTAGGTCTGTTGACAATACT ATGCATTGTGAAGAGCCCATTTGTGGTACAGAGACTTCCTTCCCATCTCCTATGGAAATACAACAGACA GAATCCTTGTTTCCATCAACAGGAATGAAACAAACCATTAATAATGGAAGGGCAGCAGCTCCTGTGGTA ATGGATGTATTACAAACAGATGTGTCTCAAAACTTTGGCTTGGAATTGGATACCAAAAGAAATGATAAT TCAGATTATTGTGGTATTTCTGAAGGTATGGAGATGAAGGTGGCACTTTCAACAACAGTGAGTGAAACC ACTGAAAGCATTTTGCAGCCTTCAATTGAGGAAGCTGATATTTTGCCAATAATGCTTTCAGAAGATAAT AACCCAAAATTTGAGCCTTCTGTTATAGTTACACCACTGGTTGAGAGTAAGTCGTGTCATCTGGAGCCT TGCTTACCTAAAGAGACTCTAGATTCTTCACTTCAGCAGACTGAGTTAATGGACCACAGAATGGCAACT GGTGAAACAAACTCAGTATATCATGATGATGATAACTCGGTTTTGAGCATTGACCTTAATCACCTGAGA CCTATTCCAGAAGCCATCAGTCCTCTGAATAGTCCAGTGAGACCTGTAGCAAAAGTTCTTAGAAATGAA AGCCCACCTCAAGTTCCAGTGTATAATAACAGTCATAAAGATGTGTTTTTACCAAATTCAGCTCATTCT ACCTCTAAGAGTCAGTCTGATCTCAATAAGGAAAATCAAAAGCCAATTTACAAATCTGACAAATGTACA GAAGCAGACACATGTAAGAATTCACCATTAGATGAATTAGAAGAAGGAGAAATTAGAAGTGATAGTGAA ACATCTAAACCACAAGAAAGTTTTGAAAAAAATTCCAAACGTAGAGTGTCAGCTGATGTGCGGAAGTCA AAGACTATCCCACGACGTGGGAAAAGTACTGTGTGTTTAGATAAAGACAGTAGGAAAACACATGTAAGA ATCCATCAGACCAATAACAAATGGAATAAAAGACCTGATAAATCTAGCAGATCTTCAAAAACAGAGAAG AAAGATAAAGTGATGAGCACTTCCAGCTTGGAAAAAATAGTTCCAATTATTGCTGTACCCTCTTCTGAA CAAGAGATCATGCACATGTTACGAATGATAAGAAAACATGTAAGAAAAAATTATATGAAATTCAAGGCA AAATTTTCATTAATACAATTTCACAGAATTATTGAGTCAGCAATTTTGAGTTTTACATCTTTAATTAAA CATCTCAACTTACACAAAATCTCTAAGTCAGTGACTACCTTACAGAAGAATCTCTGTGATATTATAGAG TCTAAACTTAAGCAAGTTAAAAAGAATGGCATAGTTGATCGTTTATTTGAACAGCAACTACCAGATATG AAAAAAAAATTGTGGAAGTTTGTAGATGACCAACTTGATTATTTGTTTGCAAAGCTTAAGAAAATCTTA GTATGTGATTCCAAAAGCTTTGGAAGAGATAGTGATGAAGGCAAACTTGAAAAAACAAGTAAACAGAAT GCACAGTATTCAAATAGTCAGAAAAGGAGTGTGGACAACTCCAACAGAGAATTGCTGAAAGAAAAATTA TCAAAATCAGAAGACCCTGTTCATTATAAGTCTTTAGTGGGATGTAAAAAATCTGAGGAAAATTATCAA GACCAAAATAACTCCAGTATTAACACTGTAAAGCATGACATTAAAAAAAATTTTAACATCTGCTTTGAT AATATAAAGAACTCTCAATCCGAAGAGCGCTCCTTGGAAGTACACTGTCCAAGCACCCCAAAGTCAGAA AAAAACGAAGGAAGCAGCATAGAGGATGCACAGACATCCCAGCATGCAACTTTGAAGCCAGAACGAAGT TTCGAGATTCTTACCGAACAGCAAGCATCGAGCCTTACTTTTAATTTAGTGAGTGATGCACAAATGGGT GAAATATTTAAAAGTTTGTTGCAAGGTTCTGATCTTTTAGACAGCAGTGTTAACTGTACTGAAAAAAGT GAGTGGGAGTTAAAGACTCCAGAGAAGCAGCTGCTAGAGACTCTTAAGTGCGAGTCTATACCAGCTTGT ACAACAGAAGAGCTAGTTTCAGGGGTGGCTTCTCCATGTCCTAAAATGATTAGTGATGATAATTGGTCA TTATTATCATCTGAAAAAGGTCCATCTCTGTCTTCAGGGCTTTCATTGCCGGTTCATCCTGATGTGTTG GATGAAAGTTGTATGTTTGAAGTGTCTACTAACCTACCTTTAAGTAAAGATAATGTGTGTAGTGTAGAA AAGAGCAAGCCCTGCGTTTCTTCCATACTTCTTGAAGATCTAGCAGTCTCTTTAACAGTACCATCGCCT CTGAAGTCAGATGGTCATCTCAGTTTTTTAAAGCCTGATATGTCGTCCAGTTCAACTCCTGAAGAAGTC ATTAGTGCTCATTTTAGTGAAGATGCCTTACTTGAGGAAGAGGATGCATCTGAGCAAGATATTCATTTA GCTCTGGAGTCTGATAATTCAAGCAGTAAATCAAGTTGTTCTTCTTCCTGGACAAGCCGATCTGTTGCT CCAGGCTTTCAGTACCACCCTAATCTACCTATGCATGCCGTCATAATGGAAAAGTCCAATGATCATTTC ATTGTGAAAATACGACGTGCAACACCATCTACCTCTTCTGGCCTTAAACAGAGTATGATGCCTGATGAA TTATTGACATCTTTGCCCAGACATGGAAAGGAAGCTGATGAAGGACCAGAGAAAGAATATATTTCATGT CAGAACACAGTTTTTAAATCTGTGGAGGAATTGGAAAACTCCAACAAAAATGTTGATGGCAGCAAGTCA ACTCATGAAGAACAGAGCTCTATGATACAAACACAGGTTCCTGATATATATGAATTTCTTAAAGATGCT TCAGATAAGATGGGTCATAGTGATGAAGTGGCTGATGAATGTTTCAAATTGCATCAAGTATGGGAAACA AAAGTGCCTGAAAGCATTGAAGAATTGCCTTCAATGGAAGAAATCTCACACTCTGTTGGGGAACATCTT CCAAACACATACGTAGATCTAACGAAAGATCCAGTCACTGAAACCAAAAACTTGGGGGAATTCATAGAA GTAACAGTTTTACATATTGATCAGTTGGGATGTTCTGGAGGCAATTTAAATCAGAGTGCTCAAATATTA GACAATTCTTTGCAGGCTGATACTGTAGGTGCTTTTATTGATTTGACACAAGATGCTTCAAGTGAGGCT AAAAGTGAAGGTAATCATCCTGCATTAGCTGTGGAAGACTTGGGATGTGGGGTGATACAGGTAGATGAA GATAATTGTAAGGAAGAAAAGGCACAAGTGGCAAACAGGCCTTTAAAATGCATTGTTGAGGAAACCTAT ATCGACTTGACCACAGAATCTCCCAGTTCATGTGAAGTAAAAAAAGATGAGTTAAAATCAGAGCCAGGA TCAAATTGTGATAACTCGGAGTTGCCTGGGACTTTGCATAATTCTCACAAAAAGAGAAGAAACATTTCT GATCTAAATCATCCTCATAAAAAACAAAGAAAGGAAACAGACTTAACTAATAAGGAAAAGACCAAGAAA CCTACCCAAGATTCTTGTGAGAATACTGAAGCTCACCAAAAGAAAGCCAGTAAGAAGAAGGCCCCTCCT GTGACTAAAGATCCCTCATCATTAAAGGCAACCCCAGGGATTAAGGATTCATCAGCAGCACTTGCCACT TCTACAAGTCTTTCTGCAAAAAATGTTATTAAAAAGAAGGGAGAAATTATCATTTTATGGACAAGAAAT GATGACCGGGAAATTTTATTGGAGTGTCAGAAAAGAGGGCCATCATTTAAAACATTTGCATATTTAGCC GCCAAGTTGGATAAAAATCCAAATCAGGTCTCAGAAAGATTCCAGCAGCTAATGAAGCTCTTTGAAAAG TCAAAATGCAGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001137668
Insert Size	5949 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001137668.1
RefSeq Size	6649 bp
RefSeq ORF	5949 bp
Locus ID	9994
UniProt ID	Q9UKL3
Protein Families	Druggable Genome
MW	222.7 kDa
Gene Summary	This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Nov 2008] Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.