BTBD18 (NM_001145101) Human Untagged Clone
CAT＃: SC325299
BTBD18 (untagged)-Human BTB (POZ) domain containing 18 (BTBD18)
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC325299 representing NM_001145101.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGCTCTCCTGCCAGTCCCAAAATCCTATACAGGAACCCCCGGTTCCTCAGGTTAGCTTTTCTGCAG CTTCATCACCAGCAGCAGAGTGATGTGTTCTGTGATGTCCTTCTGCAGGCAGAAGGTGAGGCAGTTCCA GCTCACTGCTGCATCCTGTCAGCTTGTAGCCCCTTCTTCACAGAGCGCCTGGAGCGGGAGAGGCCAGCT CAGGGTGGGAAGGTGGTGCTAGAGCTGGGTGGCCTGAAGATCAGCACACTTAGGAAGCTGGTGGACTTC TTGTATACCTCAGAAATGGAAGTATCTCAAGAAGAAGCCCAGGATGTGCTATCTGCTGCCCGTCAGCTC CGTGTGTCTGAGCTGGAATCCCTTCAGCTTGAGGGTGGAAAGTTGGTGAAGGCCCCACAGGGCCGAAGG CTGAACCGAGAGTGCTTACAACCAACAAGTGCTGCACCAATCTCTGCCAGAGTGGTGACACCCAGCCAC CACCCTCACACCCCACTGCCCACTAATCAAACTCCTTGTCCTCTTGGGGCAATAAGATTGAAGTCCTTG GGGAAGGAAGAGGGGCCCCAGGAAAACAACCGACAGAATGCAGACAATTTGTCTGGCACTCTTCTGCTC AAGAGGAAGGCCAGAGCCTGCCCAACTCCACAAGAAAAAAACTCTTCACCATCAAGCCATAGTCAAGAG CCTAGAGAGAACAAGAATGACACTGCCCTTGATCCTACAGTGCTCTCCCCACCCAGCTTGTACCCCTCT GTGGACAAACACCTGTTGCCCAGAAAGATCAGGCTCAGTCGCTCAAAGCCATCTCCTGGTATCTGTACA TCAAAGCCTTCCAGCATTTTAAGTGGATCTAGCTCAGTGCCTGCAACCCCTGGCCGGCGTCTTTGGCGG CAGAGGAGTGTAAATAAAGAAACACCAGAGGACAAGCCAAAACCAGGCAGAGCTAGTCCTCTGCAGAGC ACCCCAAACCCATCTGGTCTGGGAAAGACAGGTGGGAGCAGGAAGCGGAGCCCTGAAGTCAGGGCACCT AACTCAGACTCTGCAGAGGAGGGGCAGGTTGGGAGAGTTAAACTTAGGAAGATTGTCAATGGGACTTGC TGGGAAGTGGTACAAGAGACTCCTCTCAAAAACACTCAAGATAGCCCCCAGATCCCAGATCCCGGAGGA GACTTCCAAGAGCCTTCAGGAACTCAGCCATTCTCCAGTAATGAGCAGGAAATGTCACCTACTAGAACA GAACTGTGTCAGGACTCCCCCATGTGCACTAAGCTACAAGACATTCTGGTCTCTGCTAGCCACTCCCCA GACCACCCAGTGGTGAAGTCAGAGTTTGAGTCCAGTCCAGAACTGGTAGAGAAGGAACCTATGTTGGCT ATTGACTGCAGAGAACCCTATGCATTTGACACAGCTCTGCTGGAGCAGCCCTGTGAGGCTGAGGAGTAC CGAATCACAAGTGCTGCTGCCACCAGTGAGCTGGAGGAGATCCTGGACTTCATGCTCTGTGGCTCAGAC ATTGAACCACCTATAGGGTCTCTGGAGAGTCCAGGGGCTGAGGGCTGCAGAACGCCTACCTACCATCTG ACAGAAACAGGAAAGAACTGGATTGAAGGGGAAGAATGGTGTCTACCAGACATGGAACTCTGGCCCAGG GAGCTCACAGAATTGGAAAAGGAACCTGCTGGTGAGAACAGAGGGCCAACTGAGCTCCTTAGCCCCCTT GTCATGCCCTCTGAGGTGAGTGAAGTGCTTTCAGTAGGAGGCCGTTGGACACCAGACCTTGAAATTACC AGCTCCCAGCCACTGGATGGTCAGGAAGACAAACTTCTCCATGTCAGCTCCCTTGATACTCCCCAGAGG TCTTATGGGGACCTCTCACCTCCCTGCTCAAACTGGGTGGAGACTGGGCTGGAAGTCTCCTTGACTACA GATGAGTTATTATACCCTTCTCCCAAGGCAGGCAAGGAGGTATCTGGTCACTCTGAACTACTAGGCTCA CTTCCTGCCAGCTCTGAAGAGGAAGAGATTGATGTGGTGGACTGGACAGCAGAGGGGAGGCTGGTACCC ACTACTGTTCCCTCCGTGTGGCCTGACCCTTCCTCAGAGTCAGAAACAGAGGTAGATATACTAACATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001145101 |
| Insert Size | 2139 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001145101.1 |
| RefSeq Size | 3061 bp |
| RefSeq ORF | 2139 bp |
| Locus ID | 643376 |
| UniProt ID | B2RXH4 |
| MW | 77.9 kDa |
| Gene Summary | Specifically required during spermatogenesis to promote expression of piRNA precursors. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons, which is essential for the germline integrity. Acts by facilitating transcription elongation at piRNA loci during pachytene.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226575 | BTBD18 (Myc-DDK-tagged)-Human BTB (POZ) domain containing 18 (BTBD18) |
CNY 5216.00 |
|
| RC226575L3 | Lenti-ORF clone of BTBD18 (Myc-DDK-tagged)-Human BTB (POZ) domain containing 18 (BTBD18) |
CNY 7700.00 |
|
| RC226575L4 | Lenti-ORF clone of BTBD18 (mGFP-tagged)-Human BTB (POZ) domain containing 18 (BTBD18) |
CNY 7700.00 |
|
| RG226575 | BTBD18 (tGFP-tagged) - Human BTB (POZ) domain containing 18 (BTBD18) |
CNY 6370.00 |
