TLE6 (NM_001143986) Human Untagged Clone
CAT＃: SC325202
TLE6 (untagged)-Human transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (TLE6), transcript variant 1
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GRG6; PREMBL |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_001143986 edited
AACTTGGCTGACCTCCGCAGCTTCCGCCCGACTCTGGCTAAAGTCTTGGAGGCTACTGCC TTGAAGATGACCTCTAGGGACCAGCCCAGACCCAAGGGCCCCCCGAAAAGCACTTCGCCT TGTCCTGGGATCTCGAACTCTGAGAGCTCTCCGACGCTGAATTATCAGGGCATTCTAAAT CGGCTCAAGCAGTTCCCCAGGTTTTCTCCTCATTTTGCTGCGGAGTTGGAGAGCATTTAC TACTCGCTGCACAAGATCCAGCAGGATGTGGCAGAACATCACAAGCAGATAGGAAACGTC TTACAGATTGTGGAGAGCTGCAGCCAACTCCAGGGTTTCCAGTCTGAGGAGGTCTCACCT GCTGAACCAGCCAGCCCTGGGACGCCCCAGCAGGTGAAGGACAAGACCCTGCAGGAGTCG AGCTTTGAGGACATCATGGCCACCAGGTCCTCCGACTGGCTCCGGCGGCCTTTGGGGGAG GACAATCAGCCGGAGACCCAGCTGTTCTGGGACAAGGAGCCTTGGTTTTGGCACGACACT CTGACCGAGCAACTCTGGCGGATTTTTGCCGGCGTCCACGATGAGAAGGCAAAGCCCAGA GACAGACAGCAGGCACCAGGCCTGGGGCAGGAAAGCAAGGCACCAGGATCCTGTGACCCA GGAACAGACCCATGTCCTGAAGATGCCTCCACCCCCAGGCCACCTGAGGCCTCCTCCAGT CCCCCTGAGGGTTCCCAAGACAGGAACACAAGTTGGGGTGTGGTCCAGGAGCCTCCTGGA AGAGCCTCTCGGTTTCTACAGTCCATATCCTGGGACCCTGAGGACTTTGAAGATGCATGG AAGAGGCCAGATGCCTTGCCCGGGCAGTCAAAGAGACTCGCCGTCCCGTGCAAACTGGAA AAGATGCGGATCTTGGCACACGGGGAGCTCGTGCTCGCCACGGCCATCAGCAGCTTCACG CGGCACGTGTTCACCTGTGGCAGAAGAGGCATCAAGGTGTGGAGCCTGACTGGACAGGTG GCTGAGGACAGGTTCCCTGAGAGCCACCTGCCTATACAGACCCCTGGGGCCTTCCTGCGC ACCTGCCTGCTGTCCTCAAACAGCAGGAGCCTGCTCACCGGTGGCTACAACCTGGCCAGC GTGAGCGTGTGGGACCTGGCGGCGCCCTCCCTGCATGTGAAGGAGCAGTTGCCCTGTGCA GGTCTCAACTGCCAGGCCCTGGATGCCAACCTGGATGCCAACCTGGCCTTCGCCAGCTTC ACCAGTGGTGTGGTCAGGATCTGGGACCTGCGGGATCAGAGTGTGGTCAGGGACCTCAAG GGTTATCCTGATGGAGTCAAGAGTATCGTGGTCAAGGGCTACAACATCTGGACTGGGGGT CCGGATGCCTGTCTGCGGTGCTGGGACCAGAGGACCATCATGAAACCTCTGGAGTACCAA TTCAAGTCTCAGATAATGAGCCTGTCCCACAGCCCCCAGGAGGACTGGGTGCTGCTGGGC ATGGCCAATGGCCAGCAGTGGCTGCAAAGCACCAGCGGGAGCCAGCGGCACATGGTGGGG CAAAAAGACAGCGTCATCCTGAGCGTCAAGTTCTCCCCCTTTGGCCAGTGGTGGGCAAGC GTTGGAATGGACGACTTCCTTGGCGTCTACAGCATGCCGGCGGGGACAAAAGTGTTCGAG GTGCCTGAGATGTCTCCAGTCACGTGCTGTGACGTCTCTTCCAACAACCGCCTCGTTGTC ACAGGCTCCGGGGAGCACGCCTCCGTGTACCAGATCACCTACTGAGGGGCCTCGCTGCTG TCATCCCACTCCGGCTCCTCTTTTCATCCCCCCCCTTCCCCCCCCCCAACAAGGGGGACA TGGTGGAGGGAAGCGGGAAGGCTCTTCTGTGGCATCGCACGATCTAGTCTGTGGTGTAGA CTGGTCGCCATCACGTGTAATAAAGCACCCGGGAAAGGCAGAGTGAAAAAAAAAAAAAAA AAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001143986 |
| Insert Size | 2000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001143986.1. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001143986.1, NP_001137458.1 |
| RefSeq Size | 2033 bp |
| RefSeq ORF | 1719 bp |
| Locus ID | 79816 |
| UniProt ID | Q9H808 |
| Protein Families | Transcription Factors |
| Gene Summary | This gene encodes a member of the Groucho/ transducin-like Enhancer of split family of transcriptional co-repressors. The encoded protein is a component of the mammalian subcortical maternal complex, which is required for preimplantation development. In mouse, knock out of this gene results in cleavage-stage embryonic arrest resulting from defective cytoplasmic F-actin meshwork formation and asymmetric cell division. In human, an allelic variant in this gene is associated with preimplantation embryonic lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016] Transcript Variant: This variant (1) represents the longer variant and it encodes the longer protein (isoform 1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227706 | TLE6 (Myc-DDK-tagged)-Human transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (TLE6), transcript variant 1 |
CNY 6816.00 |
|
| RC227706L3 | Lenti-ORF clone of TLE6 (Myc-DDK-tagged)-Human transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (TLE6), transcript variant 1 |
CNY 5890.00 |
|
| RC227706L4 | Lenti-ORF clone of TLE6 (mGFP-tagged)-Human transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (TLE6), transcript variant 1 |
CNY 5890.00 |
|
| RG227706 | TLE6 (tGFP-tagged) - Human transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila) (TLE6), transcript variant 1 |
CNY 4370.00 |
