Slap (SLA) (NM_001045556) Human Untagged Clone
CAT＃: SC324569
SLA (untagged)-Human Src-like-adaptor (SLA), transcript variant 1
CNY 2400.00
CNY 6270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SLA1; SLAP |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_001045556.1
GCCAGAAAAATGATGCACGATGCTCTCTAAACTGGGTCATTCTCCACTTGGAGGGCTCAG
GGCACGGTTGACTTTCCCCGTCTGTCTCCTATACCACAGGCTCTGGGCATCACCAGCGGC CCCAGGGAAAAAGAAAGAAATGGGAAACAGCATGAAATCCACCCCTGCGCCTGCCGAGAG GCCCCTGCCCAACCCGGAGGGACTGGATAGCGACTTCCTTGCCGTGCTAAGTGACTACCC GTCTCCTGACATCAGCCCCCCGATATTCCGCCGAGGGGAGAAACTGCGTGTGATTTCTGA TGAAGGGGGCTGGTGGAAAGCTATTTCTCTTAGCACTGGTCGAGAGAGTTACATCCCTGG AATATGTGTGGCCAGAGTTTACCATGGCTGGCTGTTTGAGGGCCTGGGCAGAGACAAGGC CGAGGAGCTGCTGCAGCTGCCAGACACAAAGGTCGGCTCCTTTATGATCAGAGAGAGTGA GACCAAGAAAGGGTTTTACTCACTGTCGGTGAGACACAGGCAGGTAAAGCATTACCGCAT TTTCCGTCTGCCCAACAACTGGTACTACATTTCCCCGAGGCTCACCTTCCAGTGCCTGGA GGACCTGGTGAACCACTATTCTGAGGTGGCTGATGGCCTGTGCTGTGTGCTCACCACGCC CTGCCTGACACAAAGCACGGCTGCCCCAGCAGTGAGGGCCTCCAGCTCACCTGTCACCTT GCGTCAGAAGACTGTGGACTGGAGGAGAGTGTCCAGACTGCAGGAGGACCCCGAGGGAAC AGAGAACCCGCTTGGGGTAGACGAGTCCCTTTTCAGCTATGGCCTTCGAGAGAGCATTGC CTCTTACCTGTCCCTGACCAGTGAGGACAACACCTCCTTTGATCGAAAGAAGAAAAGCAT CTCCCTGATGTATGGTGGCAGCAAGAGAAAGAGCTCATTCTTCTCATCACCACCTTACTT TGAGGACTAGCCAAGAACAGACACAATGGTTCATGCCCAAAAGGAACAGAAGTTCCAACT ATTGCCTGGGATCTTGCGAAAAGCGAGGTTCCCTGATCCCTGGGAGCCTCACGTATTTTA GAAGCCAAGAGAAGCCACATGGAGACTCAAATTCGCATCTTCTCTATCCACATCATGACC AAAGGAACCCCTCCCTGGTGTCTGATCAGGGCTGTGGCATCACGAAACATTGGATCATGA CATGTCGGGCGATGCTTGGAAGAGCCCAGCATGTATGTATGCACACATTGTGTGTGTGGG AAGGACAAAGCCACTCTCACAAGAAAGGGCACCAGGACTGCTCTCCAAGGAACTGGACCT GTCCAGACAGTTACACTCCAAGGTCATTGGAGAGAACTTCTGTATGGGCAAGCCTGAGAG GGAGAGGAAACAAAAGCTGTGTCCTGGCAGAAGGTCTGGGTTTGCAGATGGGTGCCCTGA ATGGAACTACTTTAACTAATCCATAGGGACTTCTGGTATGCTTTCCTCTCTTTTTAAAGG AACTTCGTGACACTAAACATTAGCCCAAAGGACTTCTTAGCCTTCAATTGGGAGATACCT TTGGTCTGCTCCTGCACCAAAGCCATATGGGTGGAAGTCAGTTGGCCTCCCTGGTTCTGC AGAGGGCCAGAAGAATGAGAGAGAGGAAGACTGCTGGCAGGGAAATCGAGGAGGCGAGAC TAGAACTGCACCAGCTTCCCTGATGTCTGCAGCCATGGCTTTGCAGCGCAGACAGAGCTT CTCTGGGATGCTGGGATTCTTGCCTGTATGAATGCATCAAGTATTCATTTATTGCCCGAA TAGGCATTGCATTAAGTCCTCTGTAAGGTGTCAGGCAAGCAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001045556 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001045556.1, NP_001039021.1 |
| RefSeq Size | 3446 bp |
| RefSeq ORF | 831 bp |
| Locus ID | 6503 |
| UniProt ID | Q13239 |
| Protein Families | Druggable Genome |
| Gene Summary | Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. Involved in the negative regulation of positive selection and mitosis of T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes isoform (a). CCDS Note: This CCDS represents a 276 aa protein that is encoded by NM_001045556.2, which is based on BX648382.1 and several locally supporting ESTs. However, it should be noted that NM_001045556.2 contains an upstream translation start codon at nts 504-506, as used in the NM_001045557.2 variant which encodes a 293 aa protein. The use of this upstream start codon in NM_001045556.2 would result in only a 15 aa ORF with a stop codon in the second exon. The short length of this upstream ORF may allow escape from nonsense-mediated mRNA decay (NMD) and translation reinitiation at the downstream start codon (nts 833-835) to encode the represented 276 aa protein, or alternatively, the weak Kozak signal at the upstream start codon may enable leaky scanning by the translation machinery and subsequent use of the downstream start codon. The 276 aa protein is consistent with reports in the literature, including PMIDs:7543898, 8825655 and 9660183. Variants encoding both the longer and shorter isoforms are also present in mouse (NM_001029841.3 and NM_009192.2, respectively). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202767 | SLA (Myc-DDK-tagged)-Human Src-like-adaptor (SLA), transcript variant 1 |
CNY 3990.00 |
|
| RC202767L3 | Lenti-ORF clone of SLA (Myc-DDK-tagged)-Human Src-like-adaptor (SLA), transcript variant 1 |
CNY 5890.00 |
|
| RC202767L4 | Lenti-ORF clone of SLA (mGFP-tagged)-Human Src-like-adaptor (SLA), transcript variant 1 |
CNY 5890.00 |
|
| RG202767 | SLA (tGFP-tagged) - Human Src-like-adaptor (SLA), transcript variant 1 |
CNY 4370.00 |
