GALE (NM_001008216) Human Untagged Clone
CAT＃: SC323855
GALE (untagged)-Human UDP-galactose-4-epimerase (GALE), transcript variant 2
CNY 3656.00
CNY 7220.00
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CNY 1999.00
CNY 2700.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SDR1E1 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_001008216.1
CGCGGGAACCCGAGCAGGACTCTCCAGTCCTCAGTCACCTTGGACAAAGAAGTGTGGATC
CTCAGATTCCATCTTTTCCAACTCCAAGGTGCCATGGCAGAGAAGGTGCTGGTAACAGGT GGGGCTGGCTACATTGGCAGCCACACGGTGCTGGAGCTGCTGGAGGCTGGCTACTTGCCT GTGGTCATCGATAACTTCCATAATGCCTTCCGTGGAGGGGGCTCCCTGCCTGAGAGCCTG CGGCGGGTCCAGGAGCTGACAGGCCGCTCTGTGGAGTTTGAGGAGATGGACATTTTGGAC CAGGGAGCCCTACAGCGTCTCTTCAAAAAGTACAGCTTTATGGCGGTCATCCACTTTGCG GGGCTCAAGGCCGTGGGCGAGTCGGTGCAGAAGCCTCTGGATTATTACAGAGTTAACCTG ACCGGGACCATCCAGCTTCTGGAGATCATGAAGGCCCACGGGGTGAAGAACCTGGTGTTC AGCAGCTCAGCCACTGTGTACGGGAACCCCCAGTACCTGCCCCTTGATGAGGCCCACCCC ACGGGTGGTTGTACCAACCCTTACGGCAAGTCCAAGTTCTTCATCGAGGAAATGATCCGG GACCTGTGCCAGGCAGACAAGACTTGGAACGCAGTGCTGCTGCGCTATTTCAACCCCACA GGTGCCCATGCCTCTGGCTGCATTGGTGAGGATCCCCAGGGCATACCCAACAACCTCATG CCTTATGTCTCCCAGGTGGCGATCGGGCGACGGGAGGCCCTGAATGTCTTTGGCAATGAC TATGACACAGAGGATGGCACAGGTGTCCGGGATTACATCCATGTCGTGGATCTGGCCAAG GGCCACATTGCAGCCTTAAGGAAGCTGAAAGAACAGTGTGGCTGCCGGATCTACAACCTG GGCACGGGCACAGGCTATTCAGTGCTGCAGATGGTCCAGGCTATGGAGAAGGCCTCTGGG AAGAAGATCCCGTACAAGGTGGTGGCACGGCGGGAAGGTGATGTGGCAGCCTGTTACGCC AACCCCAGCCTGGCCCAAGAGGAGCTGGGGTGGACAGCAGCCTTAGGGCTGGACAGGATG TGTGAGGATCTCTGGCGCTGGCAGAAGCAGAATCCTTCAGGCTTTGGCACGCAAGCCTGA GGACCCTCCCCTACCAAGGACCAGGAAAAGCAGCAGCTGCCTGCTCTCCAGCCTCTGGCA GGAACTCAGGGCCCTGGAGCTGCTGGGGCCAAGCCAAGGCCTCCCCTACCTCAAACCCCA GCTGGGCCCGCTTAGCCCACCAGGCATGAGGCCAAGGGCTCCACTGACCAGGAGGCCGAG GTCTCTAACTCTTATCTTCCACAGGGTCCAAGAGTTCATCAGGACCCCCAAGAGTGAGTG AGGGGGCAAGGCTCTGGCACAAAACCTCCTCCTCCCAGGCACTCATTTATATTGCTCTGA AAGAGCTTTCCAAAGTATTTAAAAATAAAAACAAGTTTTCTTACACTGGGAAAAAAAAAA AAAAAAAAA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_001008216 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001008216.1, NP_001008217.1 |
| RefSeq Size | 1585 bp |
| RefSeq ORF | 1047 bp |
| Locus ID | 2582 |
| UniProt ID | Q14376 |
| Protein Families | Druggable Genome |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Metabolic pathways |
| Gene Summary | This gene encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and cognitive disability, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208709 | GALE (Myc-DDK-tagged)-Human UDP-galactose-4-epimerase (GALE), transcript variant 2 |
CNY 3656.00 |
|
| RC208709L3 | Lenti ORF clone of Human UDP-galactose-4-epimerase (GALE), transcript variant 2, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC208709L4 | Lenti ORF clone of Human UDP-galactose-4-epimerase (GALE), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RG208709 | GALE (tGFP-tagged) - Human UDP-galactose-4-epimerase (GALE), transcript variant 2 |
CNY 5256.00 |
