Myosin IIIB (MYO3B) (NM_138995) Human Untagged Clone
CAT＃: SC323595
MYO3B (untagged)-Kinase deficient mutant (K44M) of Human myosin IIIB (MYO3B), transcript variant 2
CNY 8904.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_138995, the custom clone sequence may differ by one or more nucleotides
ATGAAACATCTGTATGGATTATTTCACTATAATCCTATGATGCTTGGACTTGAATCACTTCCAGATCCCA CAGACACCTGGGAAATTATAGAGACCATTGGTAAAGGCACCTATGGCAAAGTCTACAAGGTAACTAACAA GAGAGATGGGAGCCTGGCTGCAGTGAAAATTCTGGATCCAGTCAGTGATATGGATGAAGAAATTGAGGCA GAATACAACATTTTGCAGTTCCTTCCTAATCATCCCAATGTTGTAAAGTTTTATGGGATGTTTTACAAAG CGGATCACTGTGTAGGGGGACAGCTGTGGCTGGTCCTGGAGCTGTGTAATGGGGGCTCAGTCACTGAGCT TGTCAAAGGTCTACTCAGATGTGGCCAGCGGTTGGATGAAGCAATGATCTCATACATCTTGTACGGGGCC CTCTTGGGCCTTCAGCATTTGCACAACAACCGAATCATCCACCGTGATGTGAAGGGGAATAACATTCTTC TGACAACAGAAGGAGGAGTTAAGCTCGTTGACTTTGGTGTTTCAGCTCAACTCACCAGTACACGTCTGCG GAGAAACACATCTGTTGGCACCCCGTTCTGGATGGCCCCTGAGGTCATTGCCTGTGAGCAGCAGTATGAC TCTTCCTATGACGCTCGCTGTGACGTCTGGTCCTTGGGGATCACAGCTATTGAACTGGGGGATGGAGACC CTCCCCTCTTTGACATGCATCCTGTGAAAACACTCTTTAAGATTCCAAGAAATCCTCCACCTACTTTACT TCATCCAGAAAAATGGTGTGAAGAATTCAACCACTTTATTTCACAGTGTCTTATTAAGGATTTTGAAAGG CGACCTTCCGTCACACATCTCCTTGACCACCCATTTATTAAAGGAGTACATGGAAAAGTTCTGTTTCTGC AAAAACAGCTGGCCAAGGTTCTCCAAGACCAGAAGCATCAAAATCCTGTTGCTAAAACCAGGCATGAGAG GATGCATACCAGAAGACCTTATCATGTGGAAGATGCTGAAAAATACTGCCTTGAGGATGATTTGGTCAAC CTAGAGGTTCTGGATGAGGATACAATTATCCATCAGTTGCAGAAACGTTATGCAGACTTGCTAATTTACA CATATGTTGGAGACATCTTAATTGCCTTAAACCCCTTCCAGAATCTAAGCATATACTCTCCACAGTTTTC CAGACTTTATCATGGGGTGAAACGCGCCTCCAATCCCCCCCACATATTTGCATCAGCAGATGCTGCTTAC CAGTGCATGGTTACTCTCAGCAAAGACCAGTGCATTGTCATCAGCGGAGAGAGTGGCTCTGGGAAGACAG AAAGCGCCCACCTGATTGTTCAGCATTTGACTTTCTTGGGAAAGGCCAATAATCAGACCTTGAGAGAGAA AATTCTACAAGTCAACTCCCTGGTGGAAGCCTTTGGGAACTCATGCACTGCCATCAATGACAACTCGAGC CGTTTTGGAAAATATCTGGAAATGATGTTTACACCAACTGGAGTTGTGATGGGGGCAAGAATCTCTGAAT ATCTCCTGGAAAAATCCAGAGTTATAAAACAGGCAGCGAGAGAGAAAAATTTTCATATATTTTACTATAT TTATGCTGGTCTTCATCACCAAAAGAAGCTTTCTGATTTCAGACTTCCTGAGGAAAAACCTCCTAGGTAC ATAGCTGATGAAACTGGAAGGGTGATGCACGACATAACTTCCAAGGAGTCTTACAGAAGACAATTCGAAG CAATTCAGCATTGCTTCAGGATTATAGGGTTCACGGACAAAGAGGTGCACTCAGTGTACAGAATTTTGGC TGGGATTTTGAATATTGGGAACATTGAGTTCGCAGCTATTTCCTCTCAACATCAGACTGATAAAAGTGAG GTGCCCAATGCTGAAGCTTTGCAAAATGCTGCCTCTGTTCTGTGCATTAGCCCTGAAGAGCTCCAGGAGG CCCTCACCTCCCACTGTGTGGTCACCCGGGGCGAGACCATCATCCGTGCCAACACTGTAGACAGGGCTGC GGACGTTCGAGACGCCATGTCCAAAGCCCTGTATGGGAGGCTCTTCAGCTGGATTGTGAATCGCATTAAT ACACTCCTGCAGCCAGACGAAAACATATGTAGTGCAGGAGGTGGAATGAATGTGGGGATCTTGGATATCT TTGGATTCGAGAATTTTCAGAGAAATTCATTTGAGCAGCTCTGCATAAACATCGCCAATGAGCAAATCCA GTACTATTTCAATCAGCATGTTTTTGCTCTTGAGCAGATGGAATATCAGAATGAAGGCATTGATGCTGTA CCCGTGGAATATGAGGACAACCGCCCGCTCTTGGACATGTTCCTCCAGAAACCCCTGGGACTGCTTGCAC TTTTGGATGAGGAAAGTCGGTTTCCCCAAGCAACTGACCAGACCCTGGTTGATAAATTTGAAGATAATCT ACGATGCAAATACTTCTGGAGGCCCAAAGGAGTGGAACTGTGCTTTGGCATTCAGCATTATGCTGGAAAG GTATTATATGATGCTTCTGGGGTTCTTGAGAAAAATAGAGACACTCTCCCTGCCGATGTGGTTGTGGTCC TGAGAACGTCAGAAAACAAGCTTCTTCAGCAGCTCTTCTCAATCCCTCTGACCAAAACAGGTAATTTGGC CCAGACAAGAGCTAGGATAACAGTGGCCTCAAGTTCTTTGCCTCCACATTTCAGTGCTGGGAAAGCCAAG GTGGACACTCTGGAGGTGATACGGCATCCGGAAGAAACCACCAACATGAAGAGGCAAACTGTGGCTTCTT ACTTCCGGTATTCTCTGATGGACCTGCTCTCCAAAATGGTGGTTGGACAGCCCCACTTTGTGCGCTGCAT TAAACCCAATGATGACCGAGAGGCCCTGCAGTTCTCTCGAGAGAGGGTGCTGGCCCAGCTCCGCTCCACA GGGATTCTGGAGACAGTCAGCATCCGCCGCCAGGGCTATTCCCACCGCATCCTTTTTGAAGAATTTGTGA AAAGGTATTATTACTTGGCATTCACAGCACATCAAACACCTCTTGCTAGCAAAGAGAGCTGTGTGGCTAT CTTGGAAAAGTCCAGATTAGATCACTGGGTACTGGGAAAAACAAAGGTTTTTCTCAAATATTACCATGTT GAGCAATTAAATTTGCTGCTTCGAGAAGTCATAGGCAGAGTGGTTGTGCTGCAGGCATATACCAAGGGGT GGCTTGGAGCCAGGAGATACAAAAGGGTCAGAGAGAAGAGAGAGAAGGGAGCCATTGCCATCCAGTCAGC CTGGAGAGGATATGATGCTCGGAGGAAATTTAAGAAAATAAGCAACAGAAGGAATGAGTCTGCTGCTCAT AATCAAGCAGGGGACACTTCAAACCAAAGCAGTGGGCCACATTCCCCCGTCGCAGCAGGTACGAGGGGAA GTGCCGAGGTTCAAGACTGCAGCGAGCCTGGTGACCATAAAGTTCTCAGGGGCTCTGTACATCGTAGGAG CCATTCACAAGCAGAATCCAACAATGGCCGTACACAGACTTCAAGCAACTCTCCTGCTGTCACAGAGAAA AATGGGCATTCACAAGCCCAGAGTTCTCCAAAAGGGTGCGATATCTTCGCAGGACATGCAAACAAGCACT CGGTTTCTGGGACTGATTTGCTGTCTTCTCGGATATGCCATCCTGCTCCAGATCAGCAAGGATTGAGTCT CTGGGGAGCCCCTCAAAAGCCTGGTTCAGAAAATGGTCTTGCACAGAAGCATCGAACACCTCGCCGACGA TGTCAGCAGCCCAAAATGCTGAGTAGCCCTGAGGACACCATGTACTATAACCAGTTAAATGGAACTCTAG AATATCAAGGGAGCAAGAGGAAGCCAAGAAAACTTGGCCAAATCAAAGTACTTGATGGGGAAGATGAATA TTACAAATCTCTGTCACCAGTGGACTGTATCCCTGAGGAGAACAACTCAGCCCACCCTTCCTTTTTTTCT TCATCCTCAAAAGGAGACTCTTTTGCTCAACATTAA >OriGene 5' read for mutant NM_138995 unedited
ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCAACAAGGAAACAT CTGTATGGATTATTTCACTATAATCCTATGATGCTTGGACTTGAATCACTTCCAGATCCCACAGACACCT GGGAAATTATAGAGACCATTGGTAAAGGCACCTATGGCAAAGTCTACAAGGTAACTAACAAGAGAGATGG GAGCCTGGCTGCAGTGATGATTCTGGATCCAGTCAGTGATATGGATGAAGAAATTGAGGCAGAATACAAC ATTTTTGCAGTTCCTTCCTAATCATCCCAATGTTTGTAAAGTTTTTATGGGGATGGTTTTAACAAAGCCG GATCACTGTGTAGGGGGACCAGCTGTGGCTGGTCCTGAGCTTGTGTAATGGGGGGCTCAGTCACTGAGCT TGTCAAGGTCTACTTAAATGTGGCAGCCGGTTGATTGAAGCCATGATTCTAAACTTCTTGTCCGGGGCCC CTCTTGGCCCTTCAGCATTGGCACAACACGGATATATCCCACCTTTGGTTGAAGGGAAATAACATCTCTT CGGCACAACAAAGGGGAGATTAAGCTGTTGACTTGGGTTTCACGCTACACTCACAATACGTCTCTGCGGA AACACATGTGTGCCCCCGCTTTGAGAGGCCCTGAGCTATCTGTAGACAGATAGACTTCTCATAACTCCGT GAACTGGCCTGGATACAATTAATGGGAGGAACCCCTTGACGCCTGGAACCTGATTCGAATTCGCAAATAT TCGGAAGTGGATTTTCGATACGCTTAAGTAGACTTGGCACTTTGACAAATTAGACCTG >SC323595 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
ACKTTGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCAACAAGGAAACATCTGTAT GGATTATTTCACTATAATCCTATGATGCTTGGACTTGAATCACTTCCAGATCCCACAGACACCTGGGAAA TTATAGAGACCATTGGTAAAGGCACCTATGGCAAAGTCTACAAGG |
| Restriction Sites | Please inquire |
| ACCN | NM_138995 |
| Insert Size | 4800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_138995.1, NP_620482.1 |
| RefSeq Size | 5560 bp |
| RefSeq ORF | 3837 bp |
| Locus ID | 140469 |
| UniProt ID | Q8WXR4 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes one of the class III myosins. Myosins are ATPases, activated by actin, that move along actin filaments in the cell. This class of myosins are characterized by an amino-terminal kinase domain and shown to be present in photoreceptors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (2) encodes the longer isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218508 | MYO3B (Myc-DDK-tagged)-Human myosin IIIB (MYO3B), transcript variant 2 |
CNY 9176.00 |
|
| RC218508L1 | Lenti-ORF clone of MYO3B (Myc-DDK-tagged)-Human myosin IIIB (MYO3B), transcript variant 2 |
CNY 11576.00 |
|
| RC218508L2 | Lenti-ORF clone of MYO3B (mGFP-tagged)-Human myosin IIIB (MYO3B), transcript variant 2 |
CNY 7600.00 |
|
| RC218508L3 | Lenti-ORF clone of MYO3B (Myc-DDK-tagged)-Human myosin IIIB (MYO3B), transcript variant 2 |
CNY 7600.00 |
|
| RC218508L4 | Lenti-ORF clone of MYO3B (mGFP-tagged)-Human myosin IIIB (MYO3B), transcript variant 2 |
CNY 7600.00 |
|
| RG218508 | MYO3B (tGFP-tagged) - Human myosin IIIB (MYO3B), transcript variant 2 |
CNY 6270.00 |
|
| SC315329 | MYO3B (untagged)-Human myosin IIIB (MYO3B), transcript variant 2 |
CNY 8904.00 |
|
| SC318603 | MYO3B (untagged)-Human myosin IIIB (MYO3B), transcript variant 2 |
CNY 10450.00 |
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