MARK1 (NM_018650) Human Untagged Clone
CAT＃: SC323553
MARK1 (untagged)-Kinase deficient mutant (K89M) of Human MAP/microtubule affinity-regulating kinase 1 (MARK1)
CNY 5560.00
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MARK; Par-1c; Par1c |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_018650, the custom clone sequence may differ by one or more nucleotides
ATGTCGGCCCGGACGCCATTGCCGACGGTGAACGAGCGGGACACGGAAAATCATACATCTGTGGATGGAT ATACTGAACCACACATCCAGCCTACCAAGTCGAGTAGCAGACAGAACATCCCCCGGTGTAGAAACTCCAT TACGTCAGCAACAGATGAACAGCCTCACATTGGAAATTACCGTTTACAAAAAACAATAGGGAAGGGAAAT TTTGCCAAAGTCAAATTGGCAAGACACGTTCTAACTGGTAGAGAGGTTGCTGTGAAAATAATAGACAAAA CTCAGCTAAATCCTACCAGTCTACAAAAGTTATTTCGAGAAGTACGAATAATGAAGATACTGAATCATCC TAATATAGTAAAATTGTTTGAAGTTATTGAAACAGAGAAGACTCTCTATTTAGTCATGGAATACGCGAGT GGGGGTGAAGTATTTGATTACTTAGTTGCCCATGGAAGAATGAAAGAGAAAGAGGCCCGTGCAAAATTTA GGCAGATTGTATCTGCTGTACAGTATTGTCATCAAAAGTACATTGTTCACCGTGATCTTAAGGCTGAAAA CCTTCTCCTTGATGGTGATATGAATATTAAAATTGCTGACTTTGGTTTTAGTAATGAATTTACAGTTGGG AACAAATTGGACACATTTTGTGGAAGCCCACCCTATGCTGCTCCCGAGCTTTTCCAAGGAAAGAAGTATG ATGGGCCTGAAGTGGATGTGTGGAGTCTGGGCGTCATTCTCTATACATTAGTCAGTGGCTCCTTGCCTTT CGATGGCCAGAATTTAAAGGAACTGCGAGAGCGAGTTTTACGAGGGAAGTACCGTATTCCCTTCTATATG TCCACAGACTGTGAAAATCTTCTGAAGAAATTATTAGTCCTGAATCCAATAAAGAGAGGCAGCTTGGAAC AAATAATGAAAGATCGATGGATGAATGTTGGTCATGAAGAGGAAGAACTAAAGCCATATACTGAGCCTGA TCCGGATTTCAATGACACAAAAAGAATAGACATTATGGTCACCATGGGCTTTGCACGAGATGAAATAAAT GATGCCTTAATAAATCAGAAGTATGATGAAGTTATGGCTACTTATATTCTTCTAGGTAGAAAACCACCTG AATTTGAAGGTGGTGAATCGTTATCCAGTGGAAACTTGTGTCAGAGGTCCCGGCCCAGTAGTGACTTAAA CAACAGCACTCTTCAGTCCCCTGCTCACCTGAAGGTCCAGAGAAGTATCTCAGCAAATCAGAAGCAGCGG CGTTTCAGTGATCATGCTGGTCCATCCATTCCTCCTGCTGTATCATATACCAAAAGACCTCAGGCTAACA GTGTGGAAAGTGAACAGAAAGAGGAGTGGGACAAAGATGTGGCTCGAAAACTTGGCAGCACAACAGTTGG ATCAAAAAGCGAGATGACTGCAAGCCCTCTTGTAGGGCCAGAGAGGAAAAAATCTTCAACTATTCCAAGT AACAATGTGTATTCTGGAGGTAGCATGGCAAGAAGGAATACATATGTCTGTGAAAGGACCACAGATCGAT ACGTAGCATTGCAGAATGGAAAAGACAGCAGCCTTACGGAGATGTCTGTGAGTAGCATATCTTCTGCAGG CTCTTCTGTGGCCTCTGCTGTCCCCTCAGCACGACCCCGCCACCAGAAGTCCATGTCCACTTCTGGTCAT CCTATTAAAGTCACACTGCCAACCATTAAAGACGGCTCTGAAGCTTACCGGCCTGGTACAACCCAGAGAG TGCCTGCTGCTTCCCCATCTGCTCACAGTATTAGTACTGCGACTCCAGACCGGACCCGTTTTCCCCGAGG GAGCTCAAGCCGAAGCACTTTCCATGGTGAACAGCTCCGGGAGCGACGCAGCGTTGCTTATAATGGGCCA CCTGCTTCACCATCCCATGAAACGGGTGCATTTGCACATGCCAGAAGGGGAACGTCAACTGGTATAATAA GCAAAATCACATCCAAATTTGTTCGCAGGGATCCAAGTGAAGGCGAAGCCAGTGGCAGAACCGACACCTC AAGAAGTACATCAGGGGAACCAAAAGAAAGAGACAAGGAAGAGGGTAAAGATTCTAAGCCGCGTTCTTTG CGGTTCACATGGAGTATGAAGACCACTAGTTCAATGGACCCTAATGACATGATGAGAGAAATCCGAAAAG TGTTAGATGCAAATAACTGTGATTATGAGCAAAAAGAGAGATTTTTGCTTTTCTGTGTCCATGGAGACGC TAGACAGGATAGCCTCGTGCAGTGGGAGATGGAAGTCTGCAAGTTGCCACGACTGTCACTTAATGGGGTT CGCTTCAAGCGAATATCTGGGACATCTATTGCCTTTAAGAACATTGCATCAAAAATAGCAAATGAGCTTA AGCTGTAA >OriGene 5' read for mutant NM_018650 unedited
CCCGCCGTTGAGCAATGGGCGGTAGGCGATGTACGGTGGGAGGCTCTATATAAGCAGAGCTCGTTTAGTG AACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCGCCGGGCA ACCGCCTCGCCCGAAGCCCTCCCTCGTTACTGTCCGCATACCCCGGCGGCGCCGCCGCGGGAAGCGGCTC CCCCTCCTCTTCCTCCGCGTCCTCTTCCCTCTTTCCCCCGCCGGGGCCGCTTGTTGCACCGCCCCGCGGC CTGCGGGAGCCGCTCGCCCCGGCCTTGTGCTCGCGTCCGCACCCCTTTCCTGTCGCCCCCCCGGGGCCCC GCACCACAGCCCGGCCGGCGAGACCCCCGGCCAGACCCCGCTGCCCGCACAAAATGTCGGCCCGGACGCC ATTGCCGACGGTGAACGAGCGGGACACGGAAATCATACATCTGTGGATGGATATACTGAACCACACATCC AGCCTACCAAGTCGAGTAGCAGACGACATCCCCCGGTGTAGAACTCATTACGTCAGCAACAGATGACAGC CTCCACATTGAATTACGGTTACCAAAAACAATAGGAGGAATTGGCCAAGTCAATTGGCAAGACCGTTCTA CTTGTAGAAGGTGCGCTGAGATGTATTATGACACAAACTTCGCTAATTCCCACCAGTGCTCTACAAGAGA AGTATCTCACCTGTGTTTCCTTACACGGTTAATTCCGAGATGTCGCAGATATTAGAGATACTCGGAACTC CTCTAATAGTGTAAATGTGTTGTAGATTTGGACACAGAGAACCCTCTAATACTCGGATATCCGGAATGTG GGGTGAGGATTTGATGACTCAATTGCCCTGAGAATGAGAGAAAGGCGCCGCTGCGCAATATGCGCATAGT TAGCCTGTCAGACAATGTAGTCCAAGTACTGTGACCCGATCTAGCTGAACCTCTCTCTATAGGGATGAAA TAATTTCGCGGCATGTGTTAAGAGTAACTG >SC323553 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AYAGAACATAGGGAGGGAATTTTGCCAAAGTCAAATTGGCAAGACACGTTCTAACTGGTAGAGAGGTTGC TGTGATGATAATAGACAAAACTCAGCTAAATCCTACCAGTCTACAAAAGAAATATATCTACCTGTTTTCT TTAACAGTTATTTCGAGAAGTACGAATAATGAAGATACTGAATCATCCTAATATAGTAAAATTGTTTGAA GTTATTGAAACAGAGAAGACTCTCTATTTAGTCATGGAATACGCG |
| Restriction Sites | Please inquire |
| ACCN | NM_018650 |
| Insert Size | 3180 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018650.2, NP_061120.2 |
| RefSeq Size | 4638 bp |
| RefSeq ORF | 2277 bp |
| Locus ID | 4139 |
| UniProt ID | Q9P0L2 |
| Domains | UBA, pkinase, TyrKc, KA1, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Serine/threonine-protein kinase (PubMed:23666762). Involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762). Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221556 | MARK1 (Myc-DDK-tagged)-Human MAP/microtubule affinity-regulating kinase 1 (MARK1) |
CNY 5824.00 |
|
| RC221556L1 | Lenti-ORF clone of MARK1 (Myc-DDK-tagged)-Human MAP/microtubule affinity-regulating kinase 1 (MARK1) |
CNY 8224.00 |
|
| RC221556L2 | Lenti-ORF clone of MARK1 (mGFP-tagged)-Human MAP/microtubule affinity-regulating kinase 1 (MARK1) |
CNY 5890.00 |
|
| RC221556L3 | Lenti-ORF clone of MARK1 (Myc-DDK-tagged)-Human MAP/microtubule affinity-regulating kinase 1 (MARK1) |
CNY 5890.00 |
|
| RC221556L4 | Lenti-ORF clone of MARK1 (mGFP-tagged)-Human MAP/microtubule affinity-regulating kinase 1 (MARK1) |
CNY 8224.00 |
|
| RG221556 | MARK1 (tGFP-tagged) - Human MAP/microtubule affinity-regulating kinase 1 (MARK1) |
CNY 7424.00 |
|
| SC113408 | MARK1 (untagged)-Human MAP/microtubule affinity-regulating kinase 1 (MARK1) |
CNY 5560.00 |
|
| SC318279 | MARK1 (untagged)-Human MAP/microtubule affinity-regulating kinase 1 (MARK1) |
CNY 5832.00 |
