BLK (NM_001715) Human Untagged Clone
CAT＃: SC323517
BLK (untagged)-Kinase deficient mutant (K269M) of Human B lymphoid tyrosine kinase (BLK)
CNY 3768.00
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MODY11 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001715, the custom clone sequence may differ by one or more nucleotides
ATGGGGCTGGTAAGTAGCAAAAAGCCGGACAAGGAAAAGCCGATCAAAGAGAAGGACAAGGGCCAATGGA GCCCCCTGAAGGTCAGCGCCCAAGACAAGGACGCCCCGCCACTGCCGCCCCTGGTTGTCTTCAACCACCT TACTCCTCCACCGCCCGATGAACACCTGGATGAAGACAAGCATTTCGTGGTGGCTCTGTATGACTACACC GCTATGAATGATCGGGACCTGCAGATGCTGAAGGGGGAGAAGCTACAGGTCCTGAAGGGAACTGGAGACT GGTGGCTGGCCAGGTCACTCGTCACAGGAAGAGAAGGCTATGTGCCCAGTAACTTTGTGGCCCGAGTGGA GAGCCTGGAAATGGAAAGGTGGTTCTTTAGATCACAGGGTCGGAAGGAGGCTGAGAGGCAGCTTCTTGCT CCAATCAACAAGGCCGGCTCCTTTCTTATCAGAGAGAGTGAAACCAACAAAGGTGCCTTCTCCCTGTCTG TGAAGGATGTCACCACCCAGGGGGAGCTGATCAAGCACTATAAGATCCGCTGCCTGGATGAAGGGGGCTA CTACATCTCCCCCCGGATCACCTTCCCCTCGCTCCAGGCCCTGGTGCAGCACTATTCTAAGAAGGGGGAT GGTCTATGCCAGAGGCTGACCCTGCCCTGTGTGCGCCCGGCCCCGCAGAATCCCTGGGCCCAGGATGAAT GGGAGATCCCCCGGCAGTCTCTCAGGCTGGTCAGGAAACTCGGGTCTGGACAATTCGGCGAAGTCTGGAT GGGTTACTACAAAAACAACATGAAGGTGGCCATTAAGACGCTGAAGGAGGGAACCATGTCTCCAGAAGCC TTTCTGGGTGAGGCCAACGTGATGAAGGCTCTGCAGCACGAGCGGCTGGTCCGACTCTACGCAGTGGTCA CCAAGGAGCCCATCTACATTGTCACCGAGTACATGGCCAGAGGATGCCTGCTGGATTTCCTGAAGACAGA TGAAGGGAGCAGATTGTCACTCCCAAGGCTGATTGACATGTCGGCGCAGATTGCTGAAGGGATGGCATAC ATTGAGCGCATGAATTCCATCCACCGCGACCTGCGGGCGGCCAACATCCTGGTGTCTGAGGCCTTGTGCT GCAAAATTGCTGATTTTGGCTTGGCTCGAATCATCGACAGTGAATACACGGCCCAAGAGGGGGCCAAGTT CCCCATCAAGTGGACAGCCCCGGAAGCCATCCACTTCGGGGTCTTCACCATCAAAGCAGACGTGTGGTCG TTTGGAGTCCTCCTGATGGAAGTTGTCACTTATGGGCGGGTGCCATACCCAGGGATGAGCAACCCCGAGG TCATCCGCAACCTGGAGCGCGGCTACCGCATGCCGCGCCCCGACACCTGCCCGCCCGAGCTGTACCGCGG CGTCATCGCCGAGTGCTGGCGCAGCCGGCCCGAGGAGCGGCCCACCTTCGAGTTCCTGCAGTCGGTGCTG GAGGACTTCTACACGGCCACCGAGCGGCAGTACGAGCTGCAGCCCTAG >OriGene 5' read for mutant NM_001715 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGGAGGCTCTGAACG CAGACCGGGGGTGCTGCTACCTCTGTCTGCTGCCGGCAGAAAGCCACAAGCCATGAAAACTGATTGAGAT GAGAAGAATTCATCTGGGACTGGCTTTTGCTTTAGGATGGTGTTGGAAGTTGCTCGTTGTCGCTAGGAGC CTGCTCCACTGTAAGGGGTGTCGGGATCTGAAGAGCTATGGGTGAAACACCACTGAAGCATTGCCAAGGT TGTCTTCAACCACCCTTACTCCTCCCACCCGCCCCGATGAACCACCCTGGATGAAAGAACAAGCATTTCT TGGTTGGGCTCTGTATTAACTCCACCCGCTATGAATGGATCCGGAACCTGCAGAATGCTGAGGGGGGGAA AACCTACGGGTCCTGAAGGAAACTGGAGACGGGTGGCTGGCAGGTCACCCTCGTACAGGGGAAAGAAAGG GCTATGTGCCCAGTAACTTTTGGGCCGAGAGGGAGAGAGCTGTGAATAGGAAAGGGGGTCTCTTTATATC CCAGGTCCCAGAGAGACTCTGAAAAGACCTTTTCTTTTCTCATATACAAAGGCCGCTCCTCTTTTTTTAT CAGAGAGTGTGAACCCACAAGTGCCCTCTCCCTCTCTGTGGAAGAGATGTCACCCCCCGGGGGGAGGTGC TCACCCCTAGATATCTGCGCCAGAAGAGGCTACTCATTATTCCCCCGATGATCATCTCTCCTCAGCGCGT GGTGCACTATTTTTAAGAGATGTGTATCTCAGTGACATCCTCGTTGTGTCGCGCGCCATACTGTCGCAGT ATGGATACTCGAGATCCTAGCAGTCGGAATCGGCTTGCAATCCGGACTCATCGGTGTACAACACACGTCG >SC323517 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TCKCTAGCACTCGGGTCTGGACATTCGGCGAAGTCTGGATGGGTTACTACAAAAACAACATGAAGGTGGC CATTATGACGCTGAAGGAGGGAACCATGTCTCCAGAAGCCTTCCTGGGTGAGGCCAACGTGATGAAGGCT CTGCAGCACGAGCGGCTGGTCCGACTCTACGCAGTGGTCACCAAGGAGCCCATCTACATTGTCACCGAGT ACATGGCCAGAGGATGCCTGCTGGATTTCCTGAAGACAGATGAAG |
| Restriction Sites | Please inquire |
| ACCN | NM_001715 |
| Insert Size | 2000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001715.2, NP_001706.2 |
| RefSeq Size | 2642 bp |
| RefSeq ORF | 1518 bp |
| Locus ID | 640 |
| UniProt ID | P51451 |
| Domains | pkinase, SH2, TyrKc, SH3, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207123 | BLK (Myc-DDK-tagged)-Human B lymphoid tyrosine kinase (BLK). Note: ORF is codon optimized |
CNY 3760.00 |
|
| RC207123L3 | Lenti-ORF clone of BLK (Myc-DDK-tagged)-Human B lymphoid tyrosine kinase (BLK). Note: ORF is codon optimized |
CNY 5890.00 |
|
| RC207123L4 | Lenti-ORF clone of BLK (mGFP-tagged)-Human B lymphoid tyrosine kinase (BLK). Note: ORF is codon optimized |
CNY 5890.00 |
|
| RG207123 | BLK (tGFP-tagged) - Human B lymphoid tyrosine kinase (BLK). Note: ORF is codon optimized |
CNY 4370.00 |
|
| SC119056 | BLK (untagged)-Human B lymphoid tyrosine kinase (BLK) |
CNY 3768.00 |
|
| SC323504 | BLK (untagged)-Kinase deficient mutant (K269M) of Human B lymphoid tyrosine kinase (BLK) |
CNY 3768.00 |
