Ataxin 7 (ATXN7) (NM_001128149) Human Untagged Clone
CAT＃: SC323239
ATXN7 (untagged)-Human ataxin 7 (ATXN7), transcript variant SCA7c
CNY 14350.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ADCAII; OPCA3; SCA7; SGF73 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC323239 representing NM_001128149.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAAGGTAGCAAGACGCCTCTCCAAAGCAGCCCTTCTGCGCAGGAGCTGAAAGCTCCACTGGAAAGA AGACATAGCTCATCCAGCAAGCCGCCTTTGGCCGTTCCTCCCACTTCAGTATTTTCCTTCTTCCCTTCT CTGTCCAAAAGCAAAGGAGGCAGTGCAAGTGGAAGCAACCGTTCTTCCAGTGGAGGTGTTCTTAGCGCA TCCTCATCAAGTTCCAAGTTGTTGAAATCACCCAAAGAGAAACTGCAGCTCAGGGGGAACACCAGGCCA ATGCATCCCATTCAGCAAAGTAGAGTTCCCCATGGTAGAATCATGACACCCTCTGTGAAAGTGGAAAAG ATTCATCCGAAAATGGATGGCACACTACTGAAATCTGCGGTGGGGCCAACCTGTCCTGCTACTGTGAGT TCCTTAGTCAAGCCTGGCCTTAACTGCCCCTCAATACCAAAGCCAACCTTGCCTTCACCTGGACAGATT CTGAATGGCAAAGGGCTTCCTGCACCGCCCACTCTGGAAAAGAAACCTGAAGACAATTCCAATAATAGG AAATTTTTAAATAAGAGATTATCAGAAAGAGAGTTTGATCCTGACATCCACTGTGGGGTTATTGATCTC GACACCAAGAAGCCCTGCACCCGGTCTTTGACATGCAAGACACATTCCTTAACCCAGCGCAGGGCTGTC CAGGGTAGAAGAAAACGATTTGATGTGTTATTAGCCGAGCACAAAAACAAAACCAGGGAAAAGGAATTG ATTCGCCATCCGGACTCTCAGCAACCACCGCAGCCTCTCAGGGACCCGCATCCCGCCCCTCCTAGAACG TCACAGGAGCCGCACCAAAACCCTCACGGAGTGATTCCTTCCGAATCAAAGCCTTTTGTAGCTAGTAAA CCTAAACCTCACACCCCCAGTCTTCCAAGGCCTCCAGGCTGCCCTGCTCAGCAAGGTGGGAGTGCCCCC ATTGACCCTCCTCCAGTCCATGAATCTCCACACCCTCCCCTGCCTGCCACTGAGCCAGCTTCTCGGTTA TCCAGTGAGGAGGGCGAAGGCGATGACAAAGAAGAGTCTGTTGAAAAACTGGACTGTCATTATTCAGGT CATCATCCTCAGCCAGCATCTTTTTGCACATTTGGGAGCCGGCAGATAGGAAGAGGCTATTACGTGTTT GACTCCAGGTGGAATCGACTTCGCTGCGCCCTCAACCTCATGGTGGAGAAGCATCTGAATGCACAGCTA TGGAAGAAAATCCCACCAGTGCCCAGTACCACCTCACCCATCTCCACACGTATTCCTCACCGGACAAAC TCTGTGCCGACATCACAATGTGGAGTCAGCTATCTGGCAGCAGCCACCGTCTCTACATCCCCAGTCCTG CTCTCATCTACCTGCATCTCCCCAAATAGCAAATCGGTACCAGCTCATGGAACCACACTAAATGCACAG CCTGCTGCTTCAGGGGCGATGGATCCTGTGTGCAGTATGCAATCCAGACAAGTGTCCTCTTCATCCTCA TCCCCTTCCACGCCCTCTGGCCTTTCCTCGGTTCCTTCCTCCCCCATGTCCAGGAAACCTCAGAAATTG AAATCCAGCAAATCTTTGAGGCCCAAGGAGTCTTCTGGTAACAGCACTAACTGTCAAAATGCCAGTAGC AGTACCAGTGGCGGCTCAGGAAAGAAACGCAAAAACAGTTCCCCACTGTTGGTTCACTCTTCCTCCTCC TCTTCCTCCTCCTCCTCTTCTTCTCATTCCATGGAGTCTTTTAGGAAAAACTGTGTGGCTCACTCTGGG CCTCCCTACCCCTCAACGGTAACATCTTCCCATAGCATCGGCCTCAACTGTGTGACGAATAAAGCAAAT GCGGTGAACGTCCGGCATGACCAGTCAGGGAGGGGCCCCCCCACCGGGAGCCCTGCTGAATCCATCAAG AGGATGAGTGTGATGGTGAACAGCAGTGATTCTACTCTTTCTCTTGGGCCATTCATTCACCAGTCCAAT GAACTGCCTGTCAACTCCCACGGCAGTTTTTCCCACTCACACACTCCTCTAGACAAACTCATAGGAAAG AAAAGAAAGTGCTCACCCAGCTCGAGCAGCATCAACAACAGCAGCAGCAAACCCACAAAGGTTGCCAAA GTGCCAGCCGTGAACAATGTCCACATGAAACACACAGGCACCATCCCAGGGGCACAAGGACTGATGAAC AGTTCCCTCCTTCATCAGCCAAAGGCACGTCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001128149 |
| Insert Size | 2244 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001128149.2 |
| RefSeq Size | 6422 bp |
| RefSeq ORF | 2244 bp |
| Locus ID | 6314 |
| UniProt ID | O15265 |
| MW | 79.5 kDa |
| Gene Summary | The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the 'pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. This locus has been mapped to chromosome 3, and it has been determined that the diseased allele associated with spinocerebellar ataxia-7 contains 37-306 CAG repeats (near the N-terminus), compared to 4-35 in the normal allele. The encoded protein is a component of the SPT3/TAF9/GCN5 acetyltransferase (STAGA) and TBP-free TAF-containing (TFTC) chromatin remodeling complexes, and it thus plays a role in transcriptional regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016] Transcript Variant: This variant (SCA7c) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant SCA7a. The encoded isoform (c) has a distinct N-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226271 | ATXN7 (Myc-DDK-tagged)-Human ataxin 7 (ATXN7), transcript variant SCA7c |
CNY 6920.00 |
|
| RC226271L3 | Lenti ORF clone of Human ataxin 7 (ATXN7), transcript variant SCA7c, Myc-DDK-tagged |
CNY 9790.00 |
|
| RC226271L4 | Lenti ORF clone of Human ataxin 7 (ATXN7), transcript variant SCA7c, mGFP tagged |
CNY 9790.00 |
|
| RG226271 | ATXN7 (tGFP-tagged) - Human ataxin 7 (ATXN7), transcript variant SCA7c |
CNY 8650.00 |
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