ATPBD3 (CTU1) (NM_145232) Human Untagged Clone

CAT#: SC322256

CTU1 (untagged)-Human cytosolic thiouridylase subunit 1 homolog (S. pombe) (CTU1)



  "NM_145232" in other vectors (5)

CNY 3656.00


货期*
现货

规格
    • 10 ug

Product images

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ATPBD3; NCS6
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for SC322256 GAGAAGCCGACCCCTCCCGCAATGCCCGCCCCGCCGTGCGCCTCCTGCCATGCTGCACGC
GCCGCCCTCCGCCGTCCGCTCTCGGGCCAAGCGCTGTGCGGTGCCTGCTTCTGCGCCGCC
TTCGAGGCCGAGGTGCTGCACACGGTGCTCGCCGGCCGCCTGCTGCCGCCCGGCGCGGTG
GTGGCCGTGGGCGCCTCGGGCGGCAAGGACTCCACGGTGCTGGCGCACGTGAGGCGCGCG
CTGGCGCCGCGCCTGGGCATCTCACTGCAGCTCGTGGCCGTCGATGAGGGCATCGGTGGC
TACCGGGACGCGGCGTTGGCGGCCGTGCGGCGCCAGGCGGTGCGCTGGGAGCTGCCGCTC
ACGGTCGTGGCCTACGAAGACCTCTTTGGGGGCTGGACGATGGACGCCGTGGCCCGCAGC
ACAGCCGGCTCCGGCCGCAGCCGCTCCTGCTGCACCTTCTGTGGAGTGCTGCGGCGCCGG
GCGCTGGAGGAAGGGGCGCGCCGCGTGGGAGCCACGCACATCGTGACAGGTCACAACGCC
GACGACATGGCGGAGACCGTGCTCATGAACTTCCTACGGGGCGACGCGGGGCGGCTGGCC
CGGGGCGGGGGCCTGGGCTCTCCCGGCGAGGGGGGCGCCCTGCCGCGCTGCCGCCCGCTG
CAGTTCGCCTCGCAGAAGGAGGTGGTGCTGTACGCGCACTTCCGCCGCCTCGACTACTTC
TCCGAGGAGTGCGTCTACGCGCCCGAGGCCTTCCGCGGCCACGCCCGGGACCTGCTCAAG
CGCCTGGAGGCGGCGCGGCCGTCCGCGGTGCTGGACCTCGTGCACTCGGCCGAGCGCCTG
GCGCTGGCCCCGGCCGCGCGGCCCCCGCGCCCCGGCGCCTGCTCCCGCTGTGGGGCGCTG
GCCAGCCGCGCGCTCTGCCAGGCCTGCGCGCTCCTGGACGGCCTGAACCGCGGCCGGCCC
CGCCTGGCCATCGGCAAGGGCCGCCGGGGTCTGGACGAGGAGGCGACGCCGGGGACGCCC
GGGGATCCGGCCCGGCCCCCCGCCTCCAAGGCCGTCCCCACCTTCTAGCGACTTCGGGCC
TCCCGCCGGGATCTGATGCCGCGGGGTGGGGGCTGCCTGTAAATGACACTGTGAATAAAC
CCGTTACCTTAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_145232
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_145232.2, NP_660275.1
RefSeq Size 1166 bp
RefSeq ORF 1047 bp
Locus ID 90353
UniProt ID Q7Z7A3
Domains ATP_bind3
Gene Summary Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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