NOB1 (NM_014062) Human Untagged Clone
CAT＃: SC321941
NOB1 (untagged)-Human NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) (NOB1)
CNY 3656.00
CNY 6650.00
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ART-4; MST158; MSTP158; NOB1P; PSMD8BP1 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_014062.1
CATGGCTCCAGTGGAGCACGTTGTGGCGGATGCTGGGGCTTTCCTGCGGCATGCGGCTCT
GCAGGACATCGGGAAGAACATTTACACCATCCGGGAGGTGGTCACTGAGATTCGGGACAA GGCCACACGCAGGCGGCTCGCTGTCCTGCCCTACGAGCTGCGGTTCAAGGAGCCCTTACC GGAATACGTGCGGCTGGTGACTGAGTTTTCAAAGAAAACAGGAGACTACCCCAGCCTCTC TGCCACGGACATCCAAGTGCTTGCACTCACATACCAGTTGGAAGCAGAGTTTGTTGGGGT GTCTCACCTAAAACAAGAACCACAGAAGGTTAAGGTGAGCTCATCGATTCAGCACCCAGA AACACCTCTGCACATTTCTGGTTTCCATCTGCCCTACAAGCCTAAACCCCCACAAGAAAC AGAAAAAGGACACTCAGCTTGTGAGCCTGAGAACCTGGAATTTAGTTCCTTCATGTTCTG GAGAAACCCTTTGCCCAACATCGATCATGAACTGCAGGAGCTGCTGATTGACAGAGGTGA GGACGTTCCAAGTGAGGAGGAGGAGGAGGAAGAAAACGGGTTTGAAGACAGAAAAGATGA CAGCGATGACGACGGGGGTGGCTGGATAACCCCCAGTAACATCAAGCAGATCCAGCAGGA GCTGGAGCAGTGTGACGTCCCCGAGGACGTGCGGGTTGGCTGCCTGACCACAGACTTCGC CATGCAGAATGTTCTGCTGCAGATGGGGCTGCACGTGCTGGCGGTGAACGGCATGCTGAT TCGTGAGGCCCGGAGCTACATCTTGCGCTGCCATGGCTGTTTCAAGACAACGTCTGACAT GAGCCGAGTGTTCTGCTCACACTGTGGGAACAAGACCCTGAAGAAAGTGTCCGTGACCGT CAGCGACGACGGCACCCTGCACATGCACTTCTCCCGCAACCCCAAGGTGCTGAACCCCCG CGGCCTCCGGTACTCGCTTCCCACTCCCAAAGGGGGCAAATACGCCATCAACCCCCATCT CACCGAGGATCAGCGCTTCCCTCAGCTGCGACTCTCCCAAAAGGCCAGGCAGAAAACCAA CGTGTTCGCCCCTGACTACATCGCCGGGGTGTCACCCTTTGTCGAGAATGACATCTCCAG CCGCTCAGCTACCCTGCAGGTCCGGGACAGCACCTTGGGAGCTGGGCGGAGACGCTTAAA TCCCAACGCTTCCAGAAAGAAGTTTGTGAAGAAAAGGTGAAGAGCGAGTTCCCGCAGGCA AATTGGATGGGCGTCTGGCCGCCGTGGAGTTCCGGTGACCCATTTCCCCAGCCGTGTCGT CTCCAGGACCACCCGATGGAAATAACAGGCGGGCTTCACGGTGCGGCTCTGTCCGCCCAT GCCCCGCTGGGTCTGCAGGGAACTGGACTGTCCCATGGCCTGTGAGCACCGGAGCGCCTG GCTGCCTGCCAAGGAAGTGCAATTGCATAAAAACAGAAAGAACAACGCCCTGGAGCCAAT CTTCAAGAAAGGAATTTCCAAAGGATAATATTTTTCTAATAAATGCGGCTGCAACCTCCT GTGCATTTAATTAAATAGGCCAAATTTTTGCTGCTTAGGTCATCTCAAGGCTGATACTTG AGCTGTGTGCCCAGAGATCATGCATTTAGATTTATATTTTTGCCAGAAAATACAAGGTTA TAATAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_014062 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014062.1, NP_054781.1 |
| RefSeq Size | 1733 bp |
| RefSeq ORF | 1239 bp |
| Locus ID | 28987 |
| UniProt ID | Q9ULX3 |
| Gene Summary | In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA (Lamanna and Karbstein, 2009 [PubMed 19706509]).[supplied by OMIM, Nov 2010] Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209537 | NOB1 (Myc-DDK-tagged)-Human NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) (NOB1) |
CNY 3656.00 |
|
| RC209537L1 | Lenti ORF clone of Human NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) (NOB1), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC209537L2 | Lenti ORF clone of Human NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) (NOB1), mGFP tagged |
CNY 6056.00 |
|
| RC209537L3 | Lenti ORF clone of Human NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) (NOB1), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC209537L4 | Lenti ORF clone of Human NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) (NOB1), mGFP tagged |
CNY 5890.00 |
|
| RG209537 | NOB1 (tGFP-tagged) - Human NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) (NOB1) |
CNY 5256.00 |
