T Plastin (PLS3) (NM_005032) Human Untagged Clone
CAT＃: SC321932
PLS3 (untagged)-Human plastin 3 (PLS3), transcript variant 1
CNY 7048.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BMND18; T-plastin |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_005032.3
ATCGTCGACCCACGCGTCCGCCCACGCGTCCGCTTTAGAGCCACAGCTGCAAAGATTCCG
AGGTGCAGAAGTTGTCTGAGTGCGTTGGTCGGCGGCAGTCGGGCCAGACCCAGGACTCTG CGACTTTACATCTTTAAATGGATGAGATGGCTACCACTCAGATTTCCAAAGATGAGCTTG ATGAACTCAAAGAGGCCTTTGCAAAAGTTGATCTCAACAGCAACGGATTCATTTGTGACT ATGAACTTCATGAGCTCTTCAAGGAAGCTAATATGCCATTACCAGGATATAAAGTGAGAG AAATTATTCAGAAACTCATGCTGGATGGTGACAGGAATAAAGATGGGAAAATAAGTTTTG ACGAATTTGTTTATATTTTTCAAGAGGTAAAAAGTAGTGATATTGCCAAGACCTTCCGCA AAGCAATCAACAGGAAAGAAGGTATTTGTGCTCTGGGTGGAACTTCAGAGTTGTCCAGCG AAGGAACACAGCATTCTTACTCAGAGGAAGAAAAATATGCTTTTGTTAACTGGATAAACA AAGCTTTGGAAAATGATCCTGATTGTAGACATGTTATACCAATGAACCCTAACACCGATG ACCTGTTCAAAGCTGTTGGTGATGGAATTGTGCTTTGTAAAATGATTAACCTTTCAGTTC CTGATACCATTGATGAAAGAGCAATCAACAAGAAGAAACTTACACCCTTCATCATTCAGG AAAACTTGAACTTGGCACTGAACTCTGCTTCTGCCATTGGGTGTCATGTTGTGAACATTG GTGCAGAAGATTTGAGGGCTGGGAAACCTCATCTGGTTTTGGGACTGCTTTGGCAGATCA TTAAGATCGGTTTGTTCGCTGACATTGAATTAAGCAGGAATGAAGCCTTGGCTGCTTTAC TCCGAGATGGTGAGACTTTGGAGGAACTTATGAAATTGTCTCCAGAAGAGCTTCTGCTTA GATGGGCAAACTTTCATTTGGAAAACTCGGGCTGGCAAAAAATTAACAACTTTAGTGCTG ACATCAAGGATTCCAAAGCCTATTTCCATCTTCTCAATCAAATCGCACCAAAAGGACAAA AGGAAGGTGAACCACGGATAGATATTAACATGTCAGGTTTCAATGAAACAGATGATTTGA AGAGAGCTGAGAGTATGCTTCAACAAGCAGATAAATTAGGTTGCAGACAGTTTGTTACCC CTGCTGATGTTGTCAGTGGAAACCCCAAACTCAACTTAGCTTTCGTGGCTAACCTGTTTA ATAAATACCCAGCACTAACTAAGCCAGAGAACCAGGATATTGACTGGACTCTATTAGAAG GAGAAACTCGTGAAGAAAGAACCTTCCGTAACTGGATGAACTCTCTTGGTGTCAATCCTC ACGTAAACCATCTCTATGCTGACCTGCAAGATGCCCTGGTAATCTTACAGTTATATGAAC GAATTAAAGTTCCTGTTGACTGGAGTAAGGTTAATAAACCTCCATACCCGAAACTGGGAG CCAACATGAAAAAGCTAGAAAACTGCAACTATGCTGTTGAATTAGGGAAGCATCCTGCTA AATTCTCCCTGGTTGGCATTGGAGGGCAAGACCTGAATGATGGGAACCAAACCCTGACTT TAGCTTTAGTCTGGCAGCTGATGAGAAGATATACCCTCAATGTCCTGGAAGATCTTGGAG ATGGTCAGAAAGCCAATGACGACATCATTGTGAACTGGGTGAACAGAACGTTGAGTGAAG CTGGAAAATCAACTTCCATTCAGAGTTTTAAGGACAAGACGATCAGCTCCAGTTTGGCAG TTGTGGATTTAATTGATGCCATCCAGCCAGGCTGTATAAACTATGACCTTGTGAAGAGTG GCAATCTAACAGAAGATGACAAGCACAATAATGCCAAGTATGCAGTGTCAATGGCTAGAA GAATCGGAGCCAGAGTGTATGCTCTCCCTGAAGACCTTGTGGAAGTAAAGCCCAAGATGG TCATGACTGTGTTTGCATGTTTGATGGGCAGGGGAATGAAGAGAGTGTAAAATAACCAAT CTGAATAAAACAGCCATGCTCCCAGGTGCATGATTCGCAGGTCAGCTATTTCCAGGTGAA GTGCTTATGGCTTAAGGAACTCTTGGCCATTCAAAGGACTTTTCATTTTGATTAACAGGA CTAGCTTATCATGAGAGCCCTCAGGGGAAAGGGTTTAAGAAAAACAACTCCTCTTTCCCA TAGTCAGAGTTGAATTTGTCAGGCACGCCTGAAATGTGCTCATAGCCAAAACATTTTACT CTCTCCTCCTAGAATGCTGCCCTTGACATTTCCCATTGCTGTATGTTATTTCTTGCTCTG TTATCTTTTGCCCTCTTAGAATGTCCCTCTCTTGGGACTTGCTTAGATGATGGGATATGA ATATTATTAGACAGTAATTTTGCTTTCCATCCAGTATGCTAGTTCTTATTCGAGAACTAT GGTCAGAGCGTATTTGGATATGAGTATCCTTTGCTTATCTTTGTAGTACTGAAAATTTGC CGAAGTAACTGGCTGTGCAGAATGTAATAGAAGCTTTTCTTATTCTTTTATTCTTAAGAT CAGTATCTTTTTACAGTATTCTTTCTACATGATCCTTTTTTGTACATTTAAGAATATTTT GATTATATTAAACAAGACTGCTGATTTTGCTACTTTTTTTAAGGGGTCTTCAAGTAAGTA AAACATACATCGTAGCTAGAAGAAAAATGTACCTTAAATTTGCATCTTCCCTCTCATACC CAAGCTGTAAACAATTGAAATATTTTGTCTTAAATCACTTGGTTCAATACATGCTTATTT GTTTTAAAACCTGTATCATCAAACTCTCTCTCTAAATTTAAAATGCTGTTGAATATGATA CTTTTGAGGAGAGAGTGTGCTCAGAACTTAGACGGGATTTGGTAGGCCAAGTATGCTAAG TGTACAATATATTTTTTAATTTTACACCTGAAACAAAGAAATGTGGTCACTAAAAATAAA AGTATATATGTAGGAATTAATGTACTCTTGCTTTGTCAAGCTGTTTGCTATAGTTTCCAA GGTATTATGTTACTCTAACTCTGAAAAGTGATGTAATCTGGTAGCAATGTAGTAGTTCAA ATAAAGGCATTTACATAATAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_005032 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005032.3, NP_005023.2 |
| RefSeq Size | 3089 bp |
| RefSeq ORF | 1893 bp |
| Locus ID | 5358 |
| UniProt ID | P13797 |
| Domains | CH, EFh |
| Gene Summary | Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010] Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1 and 2 encode the same isoform. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208934 | PLS3 (Myc-DDK-tagged)-Human plastin 3 (PLS3), transcript variant 1 |
CNY 7504.00 |
|
| RC208934L3 | Lenti-ORF clone of PLS3 (Myc-DDK-tagged)-Human plastin 3 (PLS3), transcript variant 1 |
CNY 6460.00 |
|
| RC208934L4 | Lenti-ORF clone of PLS3 (mGFP-tagged)-Human plastin 3 (PLS3), transcript variant 1 |
CNY 9904.00 |
|
| RG208934 | PLS3 (tGFP-tagged) - Human plastin 3 (PLS3), transcript variant 1 |
CNY 9104.00 |
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