LIN54 (NM_001115007) Human Untagged Clone
CAT＃: SC318923
LIN54 (untagged)-Human lin-54 homolog (C. elegans) (LIN54), transcript variant 2
CNY 8460.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CXCDC1; JC8.6; MIP120; TCX1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318923 representing NM_001115007.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGGTGGTGCCAGCTGAGATTGCTAAGAAGCCTCGAACGCCAACCTCTGGTCCAGTAATCACGAAG CTGATCTTTGCAAAACCAATTAATAGTAAAGCAGTTACAGGACAGACAACTCAAGTTTCACCACCAGTC ATTGCAGGTAGGGTTCTTTCACAGTCTACTCCCGGAACTCCATCAAAGACCATAACAATATCTGAAAGT GGTGTTATTGGATCAACTTTAAATTCTACAACACAGACACCAAATAAAATAGCCATCTCACCTTTGAAA TCGCCAAATAAGGCAGTGAAATCAACTGTGCAGACCATCACTGTTGGAGGAGTGAGCACATCACAGTTT AAGACAATTATTCCTCTGGCAACTGCTCCCAATGTCCAGCAGATTCAAGTGCCTGGAAGCAAGTTTCAT TATGTCCGACTTGTTACTGCCACATCAGCCAGTAGCTCAACCCAGCCAGTTAGTCAGAATCCCAGTACA AACACTCAGCCTCTTCAGCAAGCAAAGCCAGTGGTTGTTAATACAACCCCAGTGCGGATGTCAGTTCCA ATTGTCTCAGCTCAGGCTGTCAAACAAGTTGTTCCAAAACCAATCAATCCAACTTCACAAATAGTAACT ACTAGCCAGCCACAGCAACGGCTTATCATGCCTGCCACACCACTGCCACAGATCCAGCCCAACCTCACT AACCTGCCACCAGGCACTGTCCTGGCACCAGCTCCGGGAACAGGGAATGTGGGTTATGCAGTGCTTCCA GCTCAGTATGTTACTCAGCTACAGCAGTCTTCATATGTATCAATAGCAAGCAACTCTACCTTTACTGGA ACATCTGGTATCCAGACCCAGGCACGGCTTCCATTCAATGGCATAATCCCATCAGAGTCGGCCAGTCGG CCCCGAAAGCCCTGTAATTGTACAAAATCACTGTGTTTGAAATTGTATTGTGATTGCTTTGCAAATGGT GAATTTTGCAACAACTGCAATTGTACTAATTGTTACAACAATTTGGAACATGAAAATGAAAGGCAAAAA GCAATAAAGGCATGCCTTGACAGAAATCCAGAAGCCTTTAAGCCTAAGATAGGGAAAGGAAAGGAGGGA GAATCTGATCGACGTCATAGCAAAGGGTGTAATTGCAAACGATCAGGATGTCTTAAAAACTACTGTGAA TGCTATGAGGCAAAAATAATGTGTTCCTCAATATGCAAATGTATTGGCTGTAAGAATTTTGAAGAAAGC CCGGAAAGGAAGACATTGATGCATTTGGCAGATGCAGCTGAAGTAAGGGTACAGCAACAAACAGCAGCC AAGACGAAGTTATCCTCTCAAATTTCAGACTTGCTTACTAGGCCAACACCAGCTTTAAATAGTGGAGGC GGAAAATTGCCATTTACATTTGTAACTAAGGAAGTAGCTGAAGCCACATGTAATTGCCTCCTTGCCCAG GCAGAGCAGGCAGACAAGAAGGGAAAATCAAAGGCAGCAGCGGAACGGATGATACTTGAGGAATTCGGA CGATGTTTGATGAGTGTCATCAACTCTGCAGGAAAGGCAAAAAGTGACCCTTGTGCCATGAATTGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001115007 |
| Insert Size | 1587 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001115007.2 |
| RefSeq Size | 5279 bp |
| RefSeq ORF | 1587 bp |
| Locus ID | 132660 |
| UniProt ID | Q6MZP7 |
| MW | 56.3 kDa |
| Gene Summary | LIN54 is a component of the LIN, or DREAM, complex, an essential regulator of cell cycle genes (Schmit et al., 2009 [PubMed 19725879]).[supplied by OMIM, Dec 2010] Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. Variants 2, 3 and 7 encode the same isoform (b) which is shorter than isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225902 | LIN54 (Myc-DDK-tagged)-Human lin-54 homolog (C. elegans) (LIN54), transcript variant 2 |
CNY 3936.00 |
|
| RC225902L3 | Lenti ORF clone of Human lin-54 homolog (C. elegans) (LIN54), transcript variant 2, Myc-DDK-tagged |
CNY 6460.00 |
|
| RC225902L4 | Lenti ORF clone of Human lin-54 homolog (C. elegans) (LIN54), transcript variant 2, mGFP tagged |
CNY 6460.00 |
|
| RG225902 | LIN54 (tGFP-tagged) - Human lin-54 homolog (C. elegans) (LIN54), transcript variant 2 |
CNY 5040.00 |
