BLNK (NM_001114094) Human Untagged Clone
CAT＃: SC318876
BLNK (untagged)-Human B-cell linker (BLNK), transcript variant 2
CNY 6940.00
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AGM4; BASH; bca; BLNK-S; LY57; SLP-65; SLP65 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318876 representing NM_001114094.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACAAGCTTAATAAAATAACCGTCCCCGCCAGTCAGAAGTTGAGGCAGCTTCAAAAGATGGTCCAT GATATTAAAAACAATGAAGGTGGAATAATGAATAAAATCAAAAAGCTAAAAGTCAAAGCACCTCCAAGT GTTCCTCGAAGGGACTACGCTTCAGAGAGCCCTGCTGACGAAGAGGAGCAGTGGTCCGATGACTTTGAC AGCGACTATGAAAATCCAGATGAGCACTCGGACTCAGAGATGTACGTGATGCCCGCCGAGGAGAACGCT GATGACAGCTACGAGCCGCCTCCAGTAGAGCAGGAAACCAGGCCGGTTCACCCAGCCCTGCCCTTCGCC AGAGGCGAGTATATAGACAATCGATCAAGCCAGAGGCATTCCCCACCCTTCAGCAAGACACTTCCCAGT AAGCCCAGCTGGCCTTCAGAGAAAGCAAGGCTCACCTCCACCCTGCCGGCCCTGACTGCTTTGCAGAAA CCTCAAGTCCCACCCAAACCCAAAGGCCTCCTTGAGGATGAGGCTGATTATGTGGTCCCCGTGGAAGAT AATGATGAAAACTATATTCATCCCACAGAAAGCAGTTCACCTCCACCTGAAAAAGGTCGAAACAGTGGG GCCTGGGAAACCAAGTCACCTCCACCAGCTGCACCATCCCCGTTGCCACGGGCCGGGAAAAAACCAACG ACACCACTGAAGACAACTCCAGTTGCCTCTCAACAGAATGCTTCAAGTGTTTGTGAAGAAAAACCTATA CCTGCTGAACGCCACCGAGGGTCAAGTCACAGACAAGAAGCTGTGCAGTCACCAGTGTTTCCTCCTGCC CAGAAACAAATCCACCAAAAACCCATACCTCTGCCAAGATTTACAGAAGGGGGAAACCCAACTGTGGAT GGGCCCCTACCCAGCTTTTCATCTAATTCCACTATTTCAGAACAGGAAGCTGGCGTTCTCTGCAAGCCA TGGTATGCTGGAGCCTGTGATCGAAAGTCTGCTGAAGAGGCATTGCACAGATCAAACAAGGATGGATCA TTTCTTATTCGGAAAAGCTCTGGCCATGATTCCAAACAACCATATACACTAGTTGTATTCTTTAATAAG CGAGTATATAATATTCCTGTGCGATTTATTGAAGCAACAAAACAATATGCCTTGGGCAGAAAGAAAAAT GGTGAAGAGTACTTTGGAAGTGTTGCTGAAATCATCAGGAATCATCAACATAGTCCTTTGGTTCTTATT GACAGTCAGAATAACACAAAAGATTCCACCAGACTGAAGTATGCAGTTAAAGTTTCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001114094 |
| Insert Size | 1302 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001114094.1 |
| RefSeq Size | 1760 bp |
| RefSeq ORF | 1302 bp |
| Locus ID | 29760 |
| UniProt ID | Q8WV28 |
| Protein Families | Druggable Genome |
| Protein Pathways | B cell receptor signaling pathway, Primary immunodeficiency |
| MW | 48.2 kDa |
| Gene Summary | This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012] Transcript Variant: This variant (2) lacks an in-frame exon in the mid-coding region compared to variant 1, resulting in a shorter isoform (2, also known as BLNK-s) compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225711 | BLNK (Myc-DDK-tagged)-Human B-cell linker (BLNK), transcript variant 2 |
CNY 5488.00 |
|
| RC225711L1 | Lenti ORF clone of Human B-cell linker (BLNK), transcript variant 2, Myc-DDK-tagged |
CNY 7888.00 |
|
| RC225711L2 | Lenti ORF clone of Human B-cell linker (BLNK), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RC225711L3 | Lenti ORF clone of Human B-cell linker (BLNK), transcript variant 2, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC225711L4 | Lenti ORF clone of Human B-cell linker (BLNK), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RG225711 | BLNK (tGFP-tagged) - Human B-cell linker (BLNK), transcript variant 2 |
CNY 4370.00 |
