ZSWIM8 (NM_015037) Human Untagged Clone
CAT＃: SC318680
ZSWIM8 (untagged)-Human KIAA0913 (KIAA0913)
CNY 35530.00
货期*
9周
规格
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KIAA0913 |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_015037, the custom clone sequence may differ by one or more nucleotides
ATGGAGCTGATGTTTGCAGAGTGGGAGGACGGAGAGCGCTTCTCATTCGAGGATTCGGAC CGTTTTGAGGAGGATTCACTCTGTTCCTTCATCTCCGAGGCCGAGAGCCTCTGCCAGAAC TGGCGGGGATGGCGCAAACAGTCAGCGGGGCCCAATTCCCCCACTGGCGGCGGTGGCGGA GGTGGCAGTGGCGGTACCAGAATGCGAGATGGACTGGTGATCCCATTGGTGGAGCTGTCA GCAAAGCAGGTGGCATTTCATATCCCATTTGAAGTGGTGGAGAAAGTTTACCCACCAGTG CCTGAGCAGCTACAGCTCCGAATTGCTTTTTGGAGCTTCCCTGAGAATGAAGAGGACATT CGGCTGTATTCGTGCCTGGCCAATGGCAGTGCGGATGAGTTTCAGCGAGGGGATCAGCTC TTCCGCATGCGGGCTGTGAAGGACCCATTGCAGATAGGGTTCCACCTGAGTGCTACAGTG GTGCCACCTCAGATGGTCCCTCCTAAAGGGGCCTACAACGTGGCTGTGATGTTTGACCGC TGCCGGGTCACTTCCTGCAGCTGTACCTGTGGGGCTGGGGCCAAATGGTGCACCCACGTC GTGGCACTCTGTCTCTTCCGCATCCACAACGCTTCTGCAGTCTGCCTGCGAGCCCCAGTC TCAGAGTCCCTGTCCCGGCTACAGAGGGACCAGCTGCAAAAGTTTGCTCAGTACCTCATC AGTGAGCTCCCTCAGCAGATCCTCCCCACAGCTCAGCGTCTCCTGGACGAACTCCTGTCT TCCCAGTCAACAGCCATCAATACAGTGTGTGGAGCTCCGGACCCCACAGCAGGGCCCTCA GCATCGGACCAGAGTACTTGGTATCTGGATGAATCGACACTCACTGACAACATCAAAAAG ACACTGCACAAGTTCTGTGGCCCCTCCCCTGTGGTCTTCAGTGATGTGAACTCCATGTAT CTGTCTTCCACGGAGCCGCCAGCCGCTGCTGAATGGGCATGTCTGCTGCGCCCTCTGAGG GGCCGTGAGCCAGAGGGCGTCTGGAACCTGCTAAGCATTGTGCGGGAGATGTTCAAGCGG AGGGACAGCAATGCTGCCCCCTTGTTGGAAATCCTCACTGACCAGTGCCTCACCTATGAA CAGATAACAGGTTGGTGGTATAGCGTACGTACCTCAGCCTCACACAGCAGTGCCAGTGGG CACACGGGCCGTAGCAACGGGCAGTCAGAGGTGGCAGCCCATGCCTGTGCCAGCATGTGT GACGAGATGGTCACACTGTGGAGGCTGGCCGTGCTGGACCCTGCACTCAGCCCCCAGCGG CGCCGGGAACTGTGTACGCAGCTGCGGCAGTGGCAACTGAAGGTGATTGAGAACGTCAAG CGGGGCCAACACAAGAAGACGCTGGAGCGGCTCTTCCCCGGCTTCCGGCCAGCGGTGGAG GCCTGCTACTTCAACTGGGAAGAGGCCTACCCACTTCCTGGTGTCACCTACAGCGGCACT GACAGGAAGCTGGCACTGTGCTGGGCCCGGGCCCTGCCCTCTCGGCCAGGTGCCTCCCGC TCTGGGGGCCTGGAGGAATCCCGGGACCGGCCCCGACCCCTTCCTACTGAGCCAGCTGTG CGGCCCAAGGAGCCTGGGACCAAGCGAAAGGGCTTGGGTGAGGGGGTCCCCTCATCACAG CGGGGTCCCCGCCGCCTCTCAGCTGAAGGGGGAGATAAAGCTCTACATAAGATGGGTCCA GGTGGGGGCAAAGCCAAGGCACTGGGTGGGGCTGGCAGTGGGAGCAAGGGCTCAGCAGGT GGCGGAAGCAAGCGACGGCTGAGCAGCGAAGACAGCTCCCTGGAGCCAGACCTGGCCGAG ATGAGCCTGGATGACAGCAGCCTGGCCCTGGGCGCAGAGGCCAGCACCTTCGGGGGATTC CCTGAGAGCCCTCCACCCTGTCCTCTCCACGGTGGCTCCCGAGGCCCTTCCACTTTCCTT CCTGAGCCCCCAGATACTTATGAAGAAGATGGTGGTGTGTACTTCTCGGAAGGGCCTGAG CCTCCCACAGCCTCTGTTGGCCCCCCTGGCCTACTGCCTGGGGATGTCTGTACCCAGGAC GACCTCCCTTCTACAGATGAGAGTGGCAATGGGCTTCCCAAAACCAAAGAGGCAGCCCCT GCAGTTGGAGAGGAGGATGATGACTACCAGGCGTACTATCTGAATGCCCAGGATGGGGCT GGGGGCGAGGAAGAGAAGGCCGAGGGCGGGGCTGGGGAGGAGCACGACCTGTTTGCTGGG CTGAAGCCACTGGAACAGGAGAGTCGCATGGAGGTACTGTTTGCCTGTGCTGAGGCCCTG CATGCGCATGGCTATAGCAGTGAGGCCTCCCGTCTCACTGTGGAGCTTGCCCAGGATCTG CTAGCCAACCCACCCGACCTCAAGGTAGAGCCGCCCCCTGCCAAGGGCAAGAAGAACAAG GTATCCACGAGCCGTCAGACCTGGGTGGCTACCAACACCCTGAGCAAGGCGGCCTTCCTG TTGACAGTGCTAAGTGAGCGTCCAGAGCACCACAACCTGGCCTTCCGAGTTGGCATGTTT GCCTTGGAGCTACAGAGGCCTCCAGCTTCTACCAAGGCCTTGGAGGTGAAGCTGGCATAC CAGGAGTCTGAGGTGGCTGCCCTGCTCAAGAAGATCCCTCTGGGTCCAAGTGAGATGAGT ACCATGCGGTGCCGGGCAGAGGAACTTCGGGAGGGGACACTCTGTGACTATCGGCCTGTG TTGCCTCTCATGCTGGCCAGTTTCATCTTTGACGTTCTCTGTGCTCCAGTGGTTTCTCCC ACAGGTTCCCGGCCCCCAAGTCGCAACTGGAACAGCGAGACACCTGGGGATGAGGAGCTT GGATTTGAAGCAGCAGTTGCTGCCTTGGGCATGAAGACAACAGTGAGCGAGGCAGAACAT CCCCTCTTATGTGAAGGCACACGTCGGGAGAAGGGTGACCTGGCATTAGCACTAATGATC ACTTACAAGGACGACCAGGCCAAGCTTAAGAAGATCTTAGACAAACTCTTGGACCGAGAG AGCCAGACACATAAGCCACAGACGCTGAGTTCTTTCTACTCATCTAGCCGCCCAACCACA GCCAGCCAGAGGTCTCCTTCAAAGCACGGGGGCCCATCTGCCCCAGGGGCCCTGCAACCA CTGACCTCAGGCTCTGCAGGGCCTGCTCAACCAGGGAGTGTGGCAGGGGCTGGGCCAGGC CCCACTGAGGGCTTCACAGAGAAGAATGTGCCTGAGAGTTCCCCACATTCCCCCTGTGAG GGTCTTCCATCTGAGGCAGCTTTGACCCCAAGGCCAGAAGGGAAGGTTCCTAGCCGCTTG GCACTTGGCAGTCGTGGAGGCTATAATGGACGGGGATGGGGGTCCCCAGGACGGCCTAAG AAGAAGCACACAGGCATGGCCAGCATTGACAGCAGTGCCCCTGAAACAACATCGGATAGT TCCCCCACCTTAAGCCGGAGACCACTTCGAGGGGGCTGGGCCCCCACCTCCTGGGGTCGA GGTCAGGACAGTGACAGCATTAGCAGCTCTTCTTCGGACTCCCTGGGCTCCTCATCCTCC AGTGGAAGTCGCCGGGCCAGTGCCAGTGGAGGAGCCCGGGCGAAGACTGTTGAAGTTGGC AGGTACAAGGGCCGCCGCCCCGAGAGTCATGCCCCTCATGTACCCAATCAGCCATCAGAG GCAGCTGCACACTTCTACTTCGAGCTGGCGAAGACAGTGCTGATCAAGGCAGGGGGCAAC AGCAGCACTTCCATTTTCACACATCCATCTTCCTCAGGGGGCCACCAGGGTCCTCACCGC AACCTGCACCTTTGCGCCTTCGAGATTGGGCTTTATGCCCTTGGCCTGCACAACTTTGTT TCTCCCAACTGGCTCTCACGTACTTATTCTTCCCACGTTTCCTGGATTACAGGCCAGGCC ATGGAGATAGGCAGCGCAGCCCTGACTATACTGGTAGAATGCTGGGATGGGCACCTGACA CCCCCTGAGGTTGCATCCCTGGCTGACAGGGCATCACGGGCAAGAGACTCCAATATGGTG AGGGCGGCAGCAGAGCTGGCCCTGAGCTGCCTGCCTCACGCCCATGCATTGAACCCTAAT GAGATCCAGCGGGCCCTGGTGCAGTGCAAGGAACAGGACAACCTGATGTTGGAGAAGGCC TGCATGGCAGTGGAAGAGGCAGCTAAGGGTGGGGGCGTGTACCCTGAAGTGTTGTTTGAG GTTGCTCACCAGTGGTTCTGGCTGTATGAGCAAACTGCAGGTGGCTCATCCACAGCCCGT GAAGGGGCTACAAGCTGTAGTGCCAGTGGGATCAGGGCAGGTGGGGAAGCTGGGCGGGGT ATGCCTGAGGGTAGAGGGGGCCCAGGGACTGAGCCGGTTACAGTGGCAGCGGCAGCAGTG ACAGCAGCAGCCACAGTGGTGCCCGTCATATCGGTGGGGTCTAGTTTATACCCGGGTCCA GGACTGGGGCATGGCCACTCCCCTGGCCTGCACCCCTACACTGCTCTACAGCCCCACCTG CCCTGTAGCCCTCAGTATCTCACTCACCCAGCTCACCCTGCCCACCCCATGCCTCACATG CCCCGGCCTGCCGTCTTCCCTGTGCCCAGCTCTGCATACCCACAGGGTGTGCATCCTGCA TTCCTAGGGGCTCAGTACCCTTATTCAGTGACTCCTCCCTCACTTGCTGCCACTGCTGTG TCTTTCCCCGTTCCTTCCATGGCACCCATCACAGTACATCCCTACCACACAGAGCCAGGG CTTCCACTGCCCACCAGTGTGGCCTGTGAGTTGTGGGGCCAGGGAACAGTGAGCAGTGTC CATCCAGCATCCACGTTTCCAGCCATCCAAGGTGCCTCACTGCCTGCCCTGACCACACAG CCCAGCCCTCTGGTGAGCGGAGGTTTTCCACCGCCCGAGGAGGAGACACACAGTCAGCCA GTCAATCCCCACAGCCTGCACCACCTGCATGCTGCCTACCGTGTCGGAATGCTGGCACTG GAGATGCTGGGTCGCCGGGCACACAACGATCACCCCAACAACTTCTCCCGCTCCCCCCCC TACACTGATGATGTCAAATGGTTGCTGGGGCTGGCAGCAAAGCTGGGAGTGAACTACGTG CACCAGTTCTGTGTGGGGGCAGCCAAGGGGGTGCTGAGCCCGTTTGTGCTGCAGGAGATC GTCATGGAGACGCTGCAGCGGCTGAGTCCCGCTCATGCCCACAACCACCTGCGTGCCCCG GCCTTCCACCAACTGGTGCAGCGCTGCCAGCAGGCATACATGCAGTACATCCACCACCGC TTGATTCACCTGACTCCTGCGGACTACGACGACTTTGTGAATGCGATCCGGAGTGCCCGC AGCGCCTTCTGCCTGACGCCCATGGGCATGATGCAGTTCAACGACATCCTACAGAACCTC AAGCGCAGCAAACAGACCAAGGAGCTGTGGCAGCGGGTCTCACTCGAGATGGCCACCTTC TCCCCC |
| Restriction Sites | Please inquire |
| ACCN | NM_015037 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015037.2, NP_055852.2 |
| RefSeq Size | 5851 bp |
| RefSeq ORF | 5529 bp |
| Locus ID | 23053 |
| UniProt ID | A7E2V4 |
| Gene Summary | Substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex that promotes target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs) (PubMed:33184234, PubMed:33184237). The SCF-like E3 ubiquitin-protein ligase complex acts by catalyzing ubiquitination and subsequent degradation of AGO proteins (AGO1, AGO2, AGO3 and/or AGO4), thereby exposing miRNAs for degradation (PubMed:33184234, PubMed:33184237). Specifically recognizes and binds AGO proteins when they are engaged with a TDMD target (PubMed:33184234). May also acts as a regulator of axon guidance: specifically recognizes misfolded ROBO3 and promotes its ubiquitination and subsequent degradation (PubMed:24012004).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes isoform (1). |
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