LIMKAIN B1 (KIAA0430) (NM_014647) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KIAA0430; LKAP; LMKB; PPP1R34
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC318664 representing NM_014647. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATGGAAGGAAACGGAACTGAGAACTCCTGCAGTAGAACACGTGGATGGCTTCAACAAGATAATGAT GCTAAGCCATGGCTTTGGAAATTCTCTAATTGCTTTTCTCGTCCTGAGCAGACGCTGCCACATAGTCCC CAAACGAAAGAGTACATGGAGAACAAGAAAGTTGCTGTGGAACTAAAGGATGTACCATCACCCCTTCAT GCTGGCTCTAAGCTTTTTCCAGCAGTCCCACTTCCTGATATTCGTTCTCTTCAGCAGCCTAAAATACAG CTTTCTTCTGTCCCCAAAGTAAGCTGCTGTGCTCATTGCCCTAATGAACCCTCCACTTCGCCAATGCGT TTTGGTGGTGGTGGTGGCGGTAGCGGAGGTACCAGTAGCTTGATTCACCCGGGCGCACTGTTAGACTCG CAAAGCACCAGGACAATCACGTGTCAGGTAGGGTCAGGGTTTGCTTTCCAGTCTGCATCTTCACTCCAG AATGCCTCAGCTAGGAACAATTTGGCAGGCATTGCAAGTGACTTTCCCAGCATGTGTCTAGAGAGTAAC CTGTCTTCCTGCAAACACCTGCCCTGTTGTGGAAAACTCCACTTCCAGTCATGTCATGGTAATGTGCAC AAGCTGCATCAGTTTCCGAGTCTGCAGGGCTGCACCTCCGCTGGCTATTTCCCCTGTTCTGATTTCACA AGCGGGGCTCCAGGGCATTTGGAAGAGCACATTTCACAGTCGGAGCTAACGCCTCACTTGTGCACCAAC TCTTTGCACCTTAATGTGGTACCTCCGGTTTGTTTAAAGGGCTCACTTTACTGCGAAGACTGTCTAAAC AAGCCAGCAAGAAATAGCATAATCGATGCGGCTAAAGTCTGGCCAAATATACCACCTCCAAATACTCAA CCTGCACCTCTTGCTGTCCCTCTGTGTAATGGCTGTGGAACCAAAGGAACAGGGAAGGAGACCACGTTG TTATTGGCGACCAGTCTAGGCAAAGCTGCTTCAAAATTTGGGTCACCAGAAGTTGCAGTAGCTGGACAG GTGCTAGAAAACTTACCCCCCATTGGAGTTTTTTGGGATATTGAAAACTGCTCCGTTCCCTCTGGCCGG TCAGCAACTGCTGTTGTGCAAAGAATCCGTGAGAAGTTTTTTAAAGGCCACAGAGAAGCAGAATTCATC TGTGTATGTGACATCAGTAAAGAAAACAAGGAAGTTATTCAAGAGCTGAATAATTGCCAGGTAACCGTT GCCCACATCAATGCTACTGCAAAGAATGCCGCTGATGATAAACTGCGGCAGAGTCTCCGCAGATTTGCA AATACACACACTGCTCCAGCCACAGTGGTTCTTGTGTCAACTGATGTCAATTTTGCATTGGAACTTAGT GACCTGAGACACAGGCATGGTTTCCACATTATTTTGGTCCATAAAAACCAGGCCTCAGAAGCACTGCTG CATCATGCTAACGAGCTGATCAGATTTGAAGAGTTCATTTCCGACTTGCCCCCCAGGTTACCACTAAAA ATGCCACAGTGCCACACTCTGCTCTATGTTTATAACCTACCAGCAAATAAGGATGGCAAGAGCGTCAGC AACAGGCTCAGACGCCTGTCCGATAATTGTGGTGGGAAAGTGCTGAGTATCACAGGCTGCAGTGCAATT CTCCGCTTCATAAACCAAGATAGTGCAGAGCGCGCTCAGAAGCGAATGGAAAACGAAGATGTCTTTGGT AATAGGATCATTGTGTCATTTACTCCAAAAAATAGAGAACTCTGTGAAACAAAGAGTTCAAATGCAATT GCTGATAAAGTGAAGTCTCCCAAAAAACTTAAGAATCCAAAATTGTGCCTCATCAAAGATGCAAGTGAA CAATCTTCCAGTGCCAAAGCCACGCCTGGAAAAGGGTCACAGGCAAATTCTGGATCTGCTACAAAAAAT ACAAATGTTAAAAGTTTACAGGAGCTGTGCCGCATGGAGTCAAAAACTGGTCATAGAAACAGTGAGCAC CAGCAAGGTCACCTGAGGCTGGTCGTACCCACTCACGGTAACTCAAGTGCTGCAGTGTCGACGCCGAAA AACTCGGGGGTGGCAGAACCCGTTTACAAAACCAGTCAGAAAAAGGAGAACCTCAGTGCCCGAAGTGTT ACCAGTTCTCCTGTAGAGAAAAAAGATAAAGAGGAGACTGTATTCCAAGTGAGTTACCCGTCTGCTTTT AGCAAGTTAGTTGCATCCAGGCAAGTCAGTCCTCTGCTCGCATCTCAGTCTTGGTCTTCTAGGAGTATG TCTCCAAACCTTTTAAACAGAGCATCCCCGCTTGCTTTCAACATTGCAAATTCGAGCAGCGAAGCCGAC TGCCCAGACCCATTTGCAAATGGTGCTGATGTCCAAGTCAGCAACATAGACTACAGATTATCCCGGAAG GAGCTGCAGCAGCTCCTGCAGGAAGCATTTGCCAGGCATGGCAAGGTGAAGAGTGTTGAGCTCAGCCCC CATACAGATTATCAACTCAAGGCTGTTGTGCAAATGGAAAACTTACAAGATGCGATCGGTGCAGTGAAT AGCCTCCACAGATACAAAATTGGCAGCAAAAAGATCCTGGTCTCACTTGCCACCGGGGCTGCCAGCAAA TCACTCTCTTTACTGAGTGCAGAAACAATGTCTGTTCTTCAGGATGCCCCTGCCTGTTGCCTGCCTCTG TTTAAATTTACAGATATCTATGAAAAAAAGTTTGGACACAAGTTGAATGTGTCAGATCTATATAAATTA ACAGACACGGTCGCAATCCGTGAACAAGGAAACGGACGGCTGGTGTGTCTCCTACCCAGCAGTCAGGCC CGCCAGAGCCCCTTGGGGTCTTCCCAGTCACACGACGGCTCCTCCACGAATTGCAGCCCAATTATATTT GAAGAGTTAGAATATCACGAGCCTGTCTGCAGACAGCATTGTTCCAATAAGGATTTCAGCGAACATGAA TTTGATCCAGACTCTTACAAGATTCCTTTTGTGATTCTTTCTTTGAAGACATTTGCGCCCCAGGTTCAC AGTCTTCTCCAGACCCACGAGGGCACCGTGCCTTTATTGAGCTTTCCAGATTGTTACATTGCAGAGTTT GGCGATCTAGAAGTAGTGCAAGAAAACCAAGGAGGTGTTCCCTTAGAACACTTCATTACCTGTGTTCCA GGTGTAAACATTGCCACTGCTCAGAATGGCATCAAAGTGGTTAAATGGATTCACAACAAGCCCCCGCCT CCCAACACTGACCCTTGGCTTCTGCGTTCGAAGAGTCCTGTAGGTAACCCCCAGCTGATCCAGTTCAGT AGAGAAGTGATTGACTTGCTGAAAAGCCAGCCATCTTGTGTCATACCCATCAGTCATTTCATCCCATCC TATCACCATCATTTTGCAAAGCAGTGCCGAGTGTCAGACTACGGATACTCCAAGCTGATTGAGTTATTA GAAGCAGTGCCTCATGTATTGCAGATTCTTGGAATGGGCTCCAAACGTCTGCTGACCCTTACCCACAGG GCCCAGGTGAAGCGCTTTACTCAGGATTTACTAAAACTTCTCAAATCCCAGGCCAGCAAACAGGTCATT GTGAGAGAATTCTCACAGGCTTATCACTGGTGTTTCTCAAAGGACTGGGATGTCACTGAATATGGTGTT TGTGAGTTGATTGACATCGTATCAGAGATTCCAGACACAACCATCTGCTTGTCCCAACAAGATAATGAA ATGGTGATTTGTATCCCCAAAAGAGAACGCACTCAGGATGAAATAGAAAGGACAAAACAGTTCTCCAAG GATGTCGTTGATTTGCTGCGTCACCAACCCCATTTCCGGATGCCCTTTAATAAATTTATCCCTTCTTAC CATCACCACTTTGGCCGGCAGTGCAAACTTGCGTACTATGGGTTTACCAAACTACTTGAACTTTTTGAA GCCATACCTGATACTTTACAAGTATTGGAATGTGGAGAAGAAAAGATCCTTACTCTGACAGAGGTGGAG CGGTTCAAAGCTCTAGCTGCCCAGTTTGTTAAACTCCTTCGGTCCCAAAAAGATAACTGCCTTATGATG ACAGATCTCCTTACAGAATATGCTAAAACTTTTGGTTATACATTTCGTCTCCAAGACTATGATGTCAGT TCCATTTCGGCTTTAACTCAGAAACTCTGCCATGTCGTGAAGGTTGCCGATATAGAATCTGGCAGACAG ATTCAGCTGATCAACCGAAAGTCTCTGCGATCTCTCACTGCCCAGTTGCTGGTATTGTTGATGTCTTGG GAAGGAACCACCCATCTTTCTGTTGAGGAGCTCAAGAGACATTACGAAAGTACCCACAACACTCCCCTT AACCCCTGTGAATATGGATTCATGACCTTGACCGAACTGCTGAAGAGCCTGCCATACTTGGTTGAGGTT TTCACTAATGATAAGATGGAAGAATGTGTGAAGCTCACAAGTCTGTATTTGTTTGCAAAGAATGTGCGG TCTTTACTTCATACTTACCACTACCAGCAGATTTTCCTTCATGAGTTTTCCATGGCCTATACCAAGTAT GTCGGAGAAACTCTGCAGCCCAAGACCTACGGCCACAGCAGCGTGGAGGAGCTCTTGGGAGCAATTCCA CAGGTGGTCTGGATAAAAGGACATGGTCATAAGAGAATTGTAGTGTTAAAAAATGACATGAAAAGTCGT TTGAGTTCACTCAGTCTCTCCCCTGCCAATCATGAAAACCAGCCCTCGGAGGGCGAGCGCATCCTGGAG GTGCCCGAATCGCACACAGCCTCGGAACTCAAGCTTGGAGCTGACGGCAGTGGGCCCAGTCACACAGAG CAGGAGCTTCTCCGCCTGACCGACGACTCCCCCGTCGACCTCCTGTGTGCGCCTGTCCCCTCGTGCCTG CCGTCCCCTCAGCTGAGACCAGACCCCGTTATCCTCCAATCTGCTGATCTCATTCAGTTTGAGGAGCGC CCTCAAGAGCCTTCTGAAATTATGATTTTAAACCAAGAAGAAAAAATGGAGATTCCAATACCAGGAAAG AGCAAAACTCTGACCTCTGACTCCAGCTCGTCCTGCATCTCAGCAGCTGTCCCCGTGCCTCCCTGCCCC TCCTCGGAAACCTCCGAGTCACTGCTCAGCAAGGACCCCGTGGAAAGCCCGGCCAAAAAGCAACCCAAA AATAGAGTCAAATTGGCAGCCAACTTTTCCTTAGCACCTATAACCAAGCTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_014647
Insert Size	5229 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_014647.3
RefSeq Size	7760 bp
RefSeq ORF	5229 bp
Locus ID	9665
UniProt ID	Q9Y4F3
MW	192.9 kDa
Gene Summary	This gene encodes a putative peroxisomal protein that appears to be conserved across Euteleostomi. In humans, it may be autoantigenic. [provided by RefSeq, Jul 2010] Transcript Variant: This variant (1) encodes isoform (1).

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