TUT7 (NM_024617) Human Untagged Clone
CAT＃: SC318636
ZCCHC6 (untagged)-Human zinc finger, CCHC domain containing 6 (ZCCHC6), transcript variant 1
CNY 28790.00
货期*
4周
规格
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PAPD6; TENT3B; ZCCHC6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318636 representing NM_024617.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGAGATACAGCAAAACCTTATTTCGTGAAGCGCACTAAAGACCGGGGGACTATGGATGATGATGAC TTCAGAAGGGGTCACCCCCAACAAGATTATTTAATAATAGATGACCATGCTAAAGGCCATGGCAGTAAA ATGGAAAAGGGCCTTCAAAAAAAGAAGATAACACCAGGGAACTATGGGAATACCCCCAGAAAAGGACCA TGTGCTGTTTCCAGCAATCCATATGCATTTAAAAACCCAATCTACAGTCAACCCGCTTGGATGAATGAC AGCCACAAAGATCAGAGTAAGAGATGGCTGTCTGATGAACATACTGGTAATTCAGACAACTGGAGAGAA TTCAAACCTGGACCTAGAATTCCTGTTATAAACCGACAAAGAAAAGACTCCTTTCAAGAAAATGAAGAT GGTTATAGGTGGCAAGACACAAGAGGCTGCAGAACTGTAAGACGACTGTTTCATAAAGACCTAACAAGC CTAGAAACCACGTCAGAAATGGAAGCAGGAAGTCCTGAAAACAAGAAGCAGAGGTCCAGACCTAGGAAG CCACGGAAGACTAGAAATGAGGAAAATGAGCAGGATGGAGACTTGGAAGGCCCTGTGATCGATGAGTCT GTACTTTCAACGAAGGAGCTGCTAGGCTTACAGCAGGCTGAGGAGAGACTGAAGAGAGACTGCATTGAC AGGCTAAAAAGGCGACCACGAAACTACCCTACAGCAAAGTACACCTGCAGACTCTGTGATGTTTTAATT GAATCCATTGCATTTGCCCATAAGCATATCAAGGAAAAGAGGCACAAGAAAAACATTAAGGAGAAGCAA GAGGAAGAGTTGCTCACTACGTTACCCCCACCAACACCCTCCCAGATAAATGCAGTTGGCATTGCCATT GACAAAGTGGTACAGGAATTTGGCTTACACAATGAGAACTTGGAACAGAGGCTGGAAATTAAACGTATC ATGGAAAATGTGTTCCAACACAAGTTACCAGATTGTTCCCTAAGATTATATGGGTCATCCTGTAGCAGA TTGGGTTTCAAAAATTCGGATGTAAACATTGACATCCAGTTTCCAGCCATTATGTCTCAGCCAGATGTC CTCTTACTTGTTCAAGAATGTTTAAAGAACAGTGACTCCTTTATTGATGTTGATGCAGACTTCCATGCT AGGGTGCCAGTGGTGGTGTGCAGAGAAAAGCAAAGTGGTCTTCTGTGTAAAGTGAGCGCAGGAAATGAA AATGCTTGTCTGACAACAAAGCATTTAACTGCCCTTGGAAAACTAGAACCAAAGCTGGTTCCTTTGGTG ATTGCATTTAGGTACTGGGCAAAGCTTTGCAGTATAGATCGCCCTGAAGAAGGAGGTCTGCCACCTTAT GTGTTTGCCCTGATGGCCATTTTCTTTCTTCAGCAGAGGAAAGAACCCCTTTTGCCTGTATATCTAGGA TCATGGATTGAAGGATTCTCATTAAGCAAACTAGGGAATTTCAACCTTCAAGACATTGAAAAAGATGTT GTGATCTGGGAACATACTGACAGTGCTGCAGGGGACACAGGCATAACAAAAGAAGAGGCACCAAGAGAA ACGCCGATTAAAAGGGGACAGGTGTCATTAATATTGGATGTGAAACACCAGCCTTCAGTACCAGTTGGG CAGCTCTGGGTGGAATTGCTGCGGTTCTATGCTTTAGAATTTAATTTGGCTGATTTAGTGATAAGTATT CGTGTCAAAGAATTGGTATCTCGGGAATTGAAGGATTGGCCCAAAAAGCGCATTGCCATTGAAGATCCC TACTCTGTTAAAAGAAATGTGGCAAGAACCCTAAATAGTCAACCTGTGTTTGAATATATACTTCATTGT TTAAGGACAACATACAAGTATTTTGCTCTTCCACACAAAATTACAAAATCCAGCCTTCTAAAGCCTCTG AATGCAATTACATGTATTTCAGAACATTCTAAAGAAGTAATAAATCATCATCCAGATGTACAAACAAAA GATGATAAGCTCAAAAACTCAGTTTTGGCCCAAGGTCCTGGTGCTACCAGTTCAGCTGCAAATACCTGT AAGGTACAGCCACTTACTCTTAAAGAGACTGCTGAAAGTTTTGGAAGCCCACCAAAAGAAGAAATGGGA AATGAACACATCAGTGTCCACCCTGAAAACTCAGACTGTATCCAAGCAGATGTTAACTCTGATGATTAC AAGGGTGATAAAGTATACCATCCAGAAACAGGAAGGAAAAACGAGAAAGAGAAAGTTGGAAGGAAGGGC AAGCATCTGTTGACTGTTGATCAGAAACGTGGAGAGCATGTTGTCTGTGGCAGCACACGTAATAATGAG TCAGAGAGCACTTTGGATTTAGAAGGCTTCCAAAATCCCACAGCTAAAGAGTGTGAGGGACTTGCCACT TTAGATAACAAGGCTGATCTTGATGGAGAAAGTACAGAAGGTACTGAGGAACTAGAAGACTCTCTAAAC CACTTTACCCACTCAGTACAGGGCCAGACATCAGAAATGATTCCCTCTGATGAAGAGGAGGAGGACGAC GAAGAAGAGGAGGAGGAAGAAGAACCTAGGCTCACCATTAACCAAAGGGAAGATGAAGATGGCATGGCT AATGAAGATGAGTTAGACAACACCTACACTGGGTCAGGGGATGAGGACGCCCTATCTGAAGAGGATGAT GAGTTAGGCGAAGCTGCTAAGTATGAAGACGTGAAAGAATGTGGAAAACATGTAGAAAGAGCTCTCCTA GTGGAACTTAATAAAATAAGTCTCAAGGAAGAAAATGTATGTGAAGAAAAAAATTCACCTGTGGATCAG TCTGATTTTTTTTATGAATTCAGTAAACTTATCTTCACCAAAGGCAAGTCTCCTACGGTAGTGTGCAGC TTATGCAAACGAGAGGGTCATCTAAAGAAGGACTGTCCTGAAGACTTCAAAAGAATCCAGCTAGAACCT CTGCCACCATTAACACCCAAGTTTTTAAATATCTTAGATCAAGTCTGTATCCAGTGTTATAAGGATTTT TCTCCAACAATTATAGAAGATCAGGCTCGTGAACATATTCGGCAAAACCTAGAAAGTTTCATAAGACAG GACTTTCCAGGAACTAAATTGAGCCTGTTTGGCTCCTCCAAAAATGGATTTGGGTTCAAACAGAGTGAC CTTGACGTCTGTATGACAATTAATGGACTTGAAACTGCTGAGGGATTGGACTGTGTCAGAACTATTGAA GAATTAGCAAGAGTCCTCAGAAAACATTCAGGTCTGAGAAACATCTTACCTATTACAACAGCAAAGGTG CCAATTGTGAAGTTCTTCCATTTGAGAAGTGGTCTGGAAGTAGATATCAGTTTGTATAACACATTGGCC CTTCATAACACAAGGCTTTTATCTGCTTATTCCGCCATTGATCCCAGAGTGAAGTATTTGTGCTATACC ATGAAAGTATTTACAAAGATGTGTGATATTGGTGATGCATCTAGAGGCAGCTTATCATCGTATGCATAT ACTCTTATGGTGCTATATTTTCTCCAGCAGAGGAATCCACCAGTCATTCCTGTCCTTCAAGAGATATAC AAAGGTGAAAAGAAACCTGAAATATTTGTTGATGGCTGGAATATTTATTTTTTTGATCAAATAGATGAA CTGCCTACCTATTGGTCAGAATGTGGAAAAAATACAGAATCTGTTGGGCAGTTATGGTTGGGCCTTCTT CGTTTCTACACAGAGGAATTTGATTTTAAAGAACATGTTATTAGCATCAGGAGAAAAAGTCTGCTTACA ACTTTTAAGAAACAGTGGACCTCAAAATACATTGTTATTGAAGATCCCTTTGATTTGAATCATAATCTT GGAGCTGGATTATCAAGGAAAATGACAAATTTTATAATGAAGGCTTTTATCAATGGTAGAAGAGTATTT GGTATTCCTGTCAAGGGATTTCCAAAGGACTACCCCTCAAAAATGGAATACTTTTTTGATCCAGATGTG TTAACTGAAGGAGAGCTGGCCCCAAATGATAGATGTTGTCGAATTTGTGGAAAAATCGGACACTTCATG AAGGACTGTCCTATGAGGAGAAAAGTAAGACGGCGGCGAGATCAGGAAGATGCCCTGAACCAAAGATAC CCTGAGAACAAGGAAAAAAGAAGCAAAGAGGACAAAGAAATTCACAACAAGTACACAGAAAGGGAGGTG TCAACAAAAGAAGATAAGCCCATACAGTGCACACCTCAGAAAGCCAAGCCAATGCGGGCAGCTGCTGAC CTGGGGAGGGAGAAGATCCTCAGGCCACCAGTAGAAAAATGGAAGAGACAGGATGACAAAGACTTAAGA GAAAAACGTTGTTTTATTTGTGGAAGAGAAGGGCACATTAAAAAGGAATGCCCACAGTTTAAAGGCTCT TCAGGTAGCCTTTCCAGTAAATATATGACTCAGGGAAAAGCCTCAGCGAAGAGGACCCAGCAGGAATCA TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_024617 |
| Insert Size | 4488 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024617.3 |
| RefSeq Size | 5696 bp |
| RefSeq ORF | 4488 bp |
| Locus ID | 79670 |
| UniProt ID | Q5VYS8 |
| Domains | zf-CCHC, PAP_assoc |
| MW | 171.2 kDa |
| Gene Summary | Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:19703396, PubMed:25480299). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (By similarity). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets (PubMed:25480299). Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (PubMed:25979828). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 uridylation is strongly enhanced in the presence of LIN28A (PubMed:22898984). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed:25979828, PubMed:28671666). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (PubMed:25979828). Does not play a role in replication-dependent histone mRNA degradation (PubMed:18172165). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed:25979828, PubMed:25480299, PubMed:19703396, PubMed:22898984, PubMed:18172165, PubMed:28671666). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (PubMed:30122351).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and it encodes the longer protein (isoform 1). Variants 1 and 2 encode the same protein isoform. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214200 | ZCCHC6 (Myc-DDK-tagged)-Human zinc finger, CCHC domain containing 6 (ZCCHC6), transcript variant 1 |
CNY 10232.00 |
|
| RC214200L3 | Lenti-ORF clone of ZCCHC6 (Myc-DDK-tagged)-Human zinc finger, CCHC domain containing 6 (ZCCHC6), transcript variant 1 |
CNY 16820.00 |
|
| RC214200L4 | Lenti-ORF clone of ZCCHC6 (mGFP-tagged)-Human zinc finger, CCHC domain containing 6 (ZCCHC6), transcript variant 1 |
CNY 16820.00 |
|
| RG214200 | ZCCHC6 (tGFP-tagged) - Human zinc finger, CCHC domain containing 6 (ZCCHC6), transcript variant 1 |
CNY 16440.00 |
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