FHOD3 (NM_025135) Human Untagged Clone
CAT＃: SC318619
FHOD3 (untagged)-Human formin homology 2 domain containing 3 (FHOD3)
CNY 27650.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CMH28; FHOS2; Formactin2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318619 representing NM_025135.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCACGCTGGCTTGCCGGGTGCAGTTCTTGGACGACACGGACCCTTTCAACAGCACCAACTTCCCC GAGCCCAGCCGGCCGCCGCTGTTCACGTTCCGCGAGGACCTCGCGCTCGGCACCCAGCTGGCGGGGGTC CATAGGCTGCTGCAGGCGCCGCACAAGCTGGATGACTGTACTCTGCAGCTCTCTCACAATGGCGCCTAC CTGGATTTGGAGGCCACCCTGGCAGAGCAGCGGGATGAGTTGGAAGGCTTCCAGGATGACGCCGGGCGG GGCAAGAAGCACAGCATCATCCTAAGGACGCAGCTGTCTGTGAGGGTCCATGCCTGCATCGAAAAACTA TACAACTCCAGCGGACGAGATTTGAGAAGGGCCCTCTTCTCCCTGAAGCAGATATTTCAGGATGACAAG GATTTGGTGCATGAATTTGTAGTGGCTGAAGGTCTGACATGTTTGATCAAGGTGGGAGCTGAGGCTGAT CAGAACTATCAGAACTACATCTTAAGGGCTTTGGGCCAGATTATGTTGTATGTGGATGGAATGAATGGA GTAATAAACCGCAATGAAACCATTCAGTGGCTGTACACTCTCATTGGGTCAAAGTTCCGCCTGGTGGTG AAGACAGCCCTGAAGCTGCTGCTCGTCTTTGTAGAGTACTCGGAGTCCAACGCACCTCTCCTAATTCAG GCTGTCACTGCTGTTGACACGAAAAGAGGGGTCAAACCTTGGTCAAATATCATGGAAATCCTGGAGGAA AAAGATGGAGTTGATACGGAGCTACTGGTTTATGCAATGACTTTGGTGAACAAGACGTTATCAGGACTA CCAGACCAAGACACCTTCTACGACGTCGTGGACTGCCTGGAGGAGCTGGGCATTGCTGCTGTGTCCCAG AGGCACTTGAACAAGAAAGGGACTGACCTGGACTTAGTGGAGCAACTCAACATTTATGAGGTGGCGCTC AGGCACGAGGATGGCGATGAGACCACGGAGCCACCCCCCAGTGGGTGCCGGGACCGGAGGAGGGCCAGC GTGTGTTCCAGTGGCGGAGGCGAGCACCGGGGCCTGGACCGCAGAAGGAGCCGCAGGCACTCGGTGCAG AGCATCAAGAGCACCCTGTCGGCCCCCACCAGTCCCTGCTCCCAGTCAGCTCCCAGCTTCAAGCCCAAC CAAGTGCGAGATCTGCGTGAAAAATACAGCAATTTTGGCAATAACTCTTATCACTCCTCAAGACCCTCA TCTGGATCCAGTGTGCCCACCACCCCCACATCATCCGTCTCACCCCCACAGGAGGCCAGGTTGGAAAGG TCATCACCGAGTGGTCTTCTCACATCATCCTTCAGGCAGCACCAAGAGTCACTGGCAGCAGAGAGAGAG AGGCGGCGGCAGGAGAGAGAAGAAAGGTTGCAGAGAATAGAGCGGGAAGAAAGAAACAAATTCAGCCGA GATTATTTAGACAAAAGAGAGGAGCAAAGGCAAGCAAGAGAAGAAAGATACAAATACTTGGAACAGTTG GCAGCTGAGGAGCACGAGAAGGAGCTGAGAAGCCGGAGTGTGAGCCGGGGCAGAGCCGACCTCTCCTTG GACCTGACCTCGCCAGCAGCCCCAGCCTGCCTGGCTCCTCTGAGCCATAGCCCCTCATCTTCAGACTCT CAAGAGGCTCTCACGGTGTCTGCCTCCTCCCCAGGAACCCCTCACCATCCCCAAGCAAGTGCCGGGGAT CCTGAACCCGAATCAGAGGCAGAACCGGAAGCAGAGGCAGGGGCGGGGCAGGTTGCTGATGAAGCTGGC CAGGACATAGCCTCTGCCCACGAGGGTGCAGAGACTGAAGTGGAGCAGGCACTAGAGCAAGAGCCGGAA GAAAGAGCCTCCCTCAGTGAAAAAGAGAGGCAGAACGAGGGGGTGAACGAGAGGGACAACTGCTCTGCC TCCAGCGTCTCGTCCTCCAGCAGCACGTTGGAGAGGGAGGAGAAGGAGGACAAGCTCTCCAGGGACAGG ACAACTGGTTTGTGGCCCGCAGGTGTCCAGGATGCAGGTGTAAATGGACAGTGTGGCGACATCCTCACC AACAAACGGTTCATGCTTGACATGCTGTATGCCCATAACAGGAAGTCTCCGGATGATGAGGAGAAGGGG GATGGGGAGGCTGGGAGGACCCAGCAGGAGGCAGAGGCGGTAGCCAGCCTTGCTACCAGGATATCCACC CTGCAGGCCAACTCTCAGACCCAGGATGAGAGTGTCAGGAGGGTGGATGTCGGCTGTTTGGACAATCGG GGCAGTGTGAAAGCATTTGCTGAGAAATTCAACAGTGGGGACCTGGGGAGAGGTTCCATCTCCCCTGAT GCTGAGCCCAATGACAAGGTCCCAGAAACAGCGCCGGTGCAGCCGAAGACAGAGTCTGATTACATCTGG GACCAGCTCATGGCCAATCCAAGAGAGCTCAGAATCCAAGACATGGATTTCACTGACCTGGGGGAGGAG GATGACATTGATGTCCTAGATGTGGACCTGGGTCACAGGGAGGCCCCTGGGCCACCTCCCCCACCCCCA CCCACCTTTCTGGGTTTGCCGCCCCCACCCCCTCCGCCCCTGTTGGACAGCATTCCTCCCCCTCCTGTC CCTGGTAATTTATTGGTTCCTCCTCCTCCAGTGTTCAACGCTCCTCAGGGCTTAGGGTGGTCCCAGGTA CCCAGGGGTCAGCCCACATTCACTAAGAAAAAGAAGACCATCCGTTTGTTCTGGAATGAAGTTCGGCCT TTTGACTGGCCATGTAAAAACAACCGACGCTGCAGAGAATTCCTGTGGTCAAAACTGGAACCCATTAAG GTGGACACTTCCAGACTGGAGCACCTGTTTGAGTCTAAATCCAAGGAACTGTCTGTCTCAAAGAAAACT GCTGCAGATGGAAAAAGGCAAGAGATCATTGTTCTGGATTCCAAGAGGAGTAACGCCATCAATATTGGT CTGACGGTGCTGCCCCCTCCAAGGACGATTAAGATCGCCATTTTGAATTTTGATGAGTATGCCTTAAAC AAAGAAGGAATCGAGAAAATTCTAACGATGATTCCCACCGATGAGGAGAAGCAGAAAATCCAGGAAGCT CAGCTGGCCAACCCTGAAATCCCCCTGGGCAGTGCAGAGCAGTTCCTCCTCACCCTGTCCTCCATCAGC GAGCTCTCTGCACGACTTCACCTCTGGGCATTCAAAATGGATTATGAAACTACAGAAAAGGAAGTAGCA GAACCACTCCTGGACCTGAAGGAAGGAATAGACCAGTTGGAGAACAATAAAACCTTGGGCTTTATCCTG TCTACTCTCTTAGCCATTGGGAACTTTCTAAATGGAACTAATGCCAAAGCGTTTGAGTTAAGCTACCTC GAGAAGGTTCCAGAAGTCAAAGACACAGTGCACAAGCAGTCGCTTCTCCACCATGTGTGCACCATGGTG GTAGAAAACTTCCCAGACAGCTCCGATCTGTACTCGGAGATCGGGGCCATCACCAGGTCAGCCAAGGTT GACTTTGATCAACTTCAGGATAATTTATGTCAGATGGAGAGAAGATGCAAAGCTTCATGGGATCACCTC AAGGCAATTGCAAAACATGAAATGAAACCAGTTTTAAAACAACGGATGTCAGAGTTCCTGAAAGACTGT GCAGAGCGAATTATAATTTTAAAGATTGTCCATAGAAGGATAATCAACAGATTCCACTCCTTTTTACTC TTTATGGGCCATCCACCTTATGCAATTCGGGAAGTGAACATAAACAAATTCTGCAGGATTATTAGTGAA TTTGCACTAGAGTATCGCACAACCAGGGAAAGGGTTTTGCAGCAGAAACAGAAACGGGCCAACCACAGA GAGAGAAATAAGACCAGAGGGAAGATGATCACCGATTCTGGCAAGTTCTCCGGCAGTTCTCCGGCGCCC CCAAGCCAGCCGCAGGGTCTGAGCTATGCGGAGGACGCGGCTGAGCACGAGAACATGAAGGCTGTGCTG AAAACCTCGTCCCCCTCCGTGGAGGACGCCACCCCCGCGCTGGGCGTCCGCACACGCAGCCGAGCAAGC CGAGGATCCACTAGTTCCTGGACTATGGGAACTGATGACTCGCCCAATGTCACAGATGATGCAGCTGAT GAGATCATGGACCGCATCGTCAAGTCAGCCACCCAAGTGCCCAGTCAGCGAGTGGTGCCGAGGGAGAGG AAACGATCCCGGGCCAACCGGAAATCTTTGCGAAGAACCCTGAAGAGCGGCCTGACCCCAGAAGAAGCC AGAGCCCTGGGCTTGGTTGGCACCTCGGAGTTGCAGCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_025135 |
| Insert Size | 4320 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025135.4 |
| RefSeq Size | 5157 bp |
| RefSeq ORF | 4320 bp |
| Locus ID | 80206 |
| UniProt ID | Q2V2M9 |
| Domains | FH2 |
| MW | 160.8 kDa |
| Gene Summary | The protein encoded by this gene is a member of the diaphanous-related formins (DRF), and contains multiple domains, including GBD (GTPase-binding domain), DID (diaphanous inhibitory domain), FH1 (formin homology 1), FH2 (formin homology 2), and DAD (diaphanous auto-regulatory domain) domains. This protein is thought to play a role in actin filament polymerization in cardiomyocytes. Mutations in this gene have been associated with dilated cardiomyopathy (DCM), characterized by dilation of the ventricular chamber, leading to impairment of systolic pump function and subsequent heart failure. Increased levels of the protein encoded by this gene have been observed in individuals with hypertrophic cardiomyopathy (HCM). Alternative splicing results in multiple transcript variants encoding different isoforms. A muscle-specific isoform has been shown to possess a casein kinase 2 (CK2) phosphorylation site at the C-terminal end of the FH2 domain. Phosphorylation of this site alters its interaction with sequestosome 1 (SQSTM1), and targets this isoform to myofibrils, while other isoforms form cytoplasmic aggregates. [provided by RefSeq, Aug 2015] Transcript Variant: This variant (1) encodes isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221215 | FHOD3 (Myc-DDK-tagged)-Human formin homology 2 domain containing 3 (FHOD3) |
CNY 9848.00 |
|
| RC221215L3 | Lenti ORF clone of Human formin homology 2 domain containing 3 (FHOD3), Myc-DDK-tagged |
CNY 12248.00 |
|
| RC221215L4 | Lenti ORF clone of Human formin homology 2 domain containing 3 (FHOD3), mGFP tagged |
CNY 16250.00 |
|
| RG221215 | FHOD3 (tGFP-tagged) - Human formin homology 2 domain containing 3 (FHOD3) |
CNY 15770.00 |
|
| SC110946 | FHOD3 (untagged)-Human formin homology 2 domain containing 3 (FHOD3) |
CNY 3656.00 |
