ARHGEF10 (NM_014629) Human Untagged Clone
CAT＃: SC318604
ARHGEF10 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 10 (ARHGEF10)
CNY 25840.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GEF10; SNCV |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318604 representing NM_014629.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACCAGCGAGAGCCCCTGCCTCCCGCTCCTGCAGAAAATGAAATGAAATATGATACCAATAATAAT GAAGAGGAAGAGGGAGAACAGTTCGATTTTGACAGTGGAGATGAAATCCCAGAAGCGGACAGACAGGCC CCATCCGCCCCTGAGACAGGAGGTGCTGGAGCCAGTGAAGCCCCTGCACCCACAGGAGGTGAGGATGGA GCTGGAGCAGAAACCACCCCAGTGGCAGAGCCTACTAAGCTGGTGCTCCCGATGAAAGTCAACCCATAT TCTGTCATCGACATCACGCCATTCCAGGAGGACCAGCCGCCCACCCCCGTGCCCAGCGCTGAGGAGGAG AATGTGGGTCTCCATGTGCCCTGCGGGTACTTGGTGCCTGTACCCTGCGGCTATGCGGTGCCCTCCAAC CTGCCCCTCCTGCTGCCCGCCTACTCCAGCCCGGTCATCATCTGCGCCACGTCCCTGGACGAAGAAGAA ACACCAGAAGTCACAGAAGATCGCCAGCCCAATTCTCTGAGTTCCGAGGAGCCTCCAACCAGTGAAGAT CAAGTCGGTCGAGAGGACAGCGCACTTGCCCGCTGGGCCGCAGACCCGGCCAACACAGCCTGGATGGAG AATCCAGAGGAAGCAATTTACGATGACGTTCCAAGGGAAAACTCAGACTCTGAACCAGATGAAATGATT TATGATGATGTTGAGAATGGGGATGAAGGTGGAAACAGCTCCTTGGAATACGGATGGAGTTCGAGTGAA TTTGAAAGTTACGAAGAGCAGAGTGACTCGGAGTGCAAGAATGGGATTCCCAGGTCCTTCCTGCGCAGC AACCACAAAAAGCAACTTTCTCATGACCTAACCCGTTTAAAGGAGCACTATGAGAAAAAGATGAGAGAT TTGATGGCAAGCACGGTGGGCGTGGTGGAGATTCAGCAGCTCAGGCAGAAGCATGAACTGAAGATGCAG AAGCTCGTGAAGGCCGCGAAGGACGGCACCAAGGACGGGCTGGAGAGGACCAGGGCAGCCGTGAAGAGG GGCCGCTCCTTCATCAGGACCAAGTCTCTCATCGCACAGGATCACAGATCTTCTCTTGAGGAAGAACAG AATTTGTTCATTGATGTTGACTGCAAGCACCCGGAAGCCATCTTGACCCCGATGCCCGAGGGTTTATCT CAGCAGCAGGTTGTAAGAAGATATATACTGGGTTCAGTTGTCGACAGTGAAAAGAACTACGTAGATGCT CTTAAGAGGATTTTGGAGCAATATGAGAAGCCGCTGTCTGAGATGGAGCCAAAGGTTCTGAGTGAGAGG AAGCTGAAGACGGTGTTCTACCGAGTCAAAGAGATCCTGCAGTGCCACTCGCTATTTCAGATCGCGCTG GCCAGCCGCGTTTCCGAGTGGGACTCCGTGGAAATGATAGGCGATGTCTTCGTGGCTTCGTTTTCTAAG TCCATGGTGCTGGATGCATACAGTGAATATGTGAACAATTTCAGCACAGCCGTGGCAGTCCTCAAGAAA ACATGTGCCACAAAGCCCGCTTTTCTTGAATTTTTAAAGCAGGAACAGGAGGCCAGCCCCGATCGAACC ACGCTCTACAGCCTGATGATGAAGCCCATCCAGAGGTTCCCACAGTTCATCCTCCTGCTCCAGGACATG CTGAAGAACACCTCCAAAGGCCACCCCGACAGGCTGCCTCTTCAGATGGCCCTGACAGAGCTCGAAACA CTAGCAGAGAAGTTAAATGAAAGAAAGAGAGATGCTGATCAACGCTGTGAAGTGAAGCAAATAGCCAAA GCCATAAACGAAAGATACCTGAACAAGCTTCTCAGCAGTGGAAGCCGATACCTCATTCGATCAGATGAT ATGATAGAAACAGTTTACAACGACAGAGGAGAGATTGTTAAAACCAAAGAACGCCGAGTCTTCATGTTA AATGATGTGTTAATGTGTGCCACCGTCAGCTCACGCCCCTCTCATGACAGCCGTGTGATGAGCAGCCAG AGGTACTTGCTGAAGTGGAGCGTTCCACTGGGACATGTGGACGCCATCGAGTATGGCAGCAGCGCAGGC ACGGGCGAGCACAGCAGGCACCTTGCCGTTCACCCGCCGGAGAGCCTGGCCGTGGTTGCTAACGCGAAA CCAAACAAAGTTTACATGGGGCCAGGACAACTGTATCAAGATTTACAAAACTTGTTGCATGACTTAAAT GTAATTGGCCAAATCACTCAGCTGATAGGAAACCTTAAAGGAAACTATCAGAACTTAAACCAGTCAGTA GCCCATGACTGGACATCAGGTTTACAAAGGCTTATTTTGAAGAAAGAAGATGAAATCAGAGCTGCGGAC TGCTGCAGAATTCAGTTACAGCTTCCCGGGAAGCAGGACAAATCTGGGCGACCGACGTTCTTTACAGCT GTGTTCAATACGTTCACCCCTGCCATCAAGGAGTCCTGGGTCAACAGCTTACAGATGGCCAAGCTCGCC CTAGAAGAGGAGAACCACATGGGCTGGTTCTGTGTGGAAGACGATGGGAATCACATTAAAAAGGAGAAG CATCCTCTCCTCGTCGGACACATGCCCGTGATGGTGGCCAAGCAGCAGGAGTTCAAGATTGAATGTGCT GCTTATAACCCTGAACCTTACCTAAATAATGAAAGCCAGCCAGATTCATTTTCCACGGCACATGGTTTC CTGTGGATCGGAAGTTGCACCCATCAAATGGGTCAGATTGCCATCGTCTCGTTTCAAAATTCCACTCCC AAAGTCATTGAGTGCTTCAACGTGGAATCTCGCATCCTGTGCATGCTGTACGTTCCCGTCGAGGAGAAG CGCAGAGAGCCTGGGGCACCCCCGGACCCCGAGACCCCGGCCGTGAGAGCTTCTGATGTCCCCACGATC TGTGTAGGGACGGAGGAGGGAAGCATTTCCATTTATAAAAGCAGTCAAGGCTCCAAGAAAGTGAGACTT CAGCACTTTTTCACTCCTGAGAAGTCCACAGTCATGAGCCTGGCTTGCACGTCTCAGAGCCTGTACGCT GGCCTGGTCAACGGGGCAGTCGCCAGCTACGCCAGAGCCCCAGATGGATCCTGGGATTCAGAACCTCAA AAAGTGATCAAGTTAGGCGTCCTACCAGTTAGAAGTCTACTCATGATGGAAGACACGTTGTGGGCGGCT TCCGGAGGTCAAGTCTTCATCATCAGTGTGGAGACTCATGCTGTAGAGGGTCAGCTGGAGGCCCACCAG GAGGAAGGCATGGTGATCTCCCACATGGCCGTGTCCGGCGTCGGGATCTGGATTGCCTTCACCTCAGGG TCCACGCTCCGCCTTTTTCACACGGAAACTCTCAAGCACCTGCAGGACATCAACATCGCCACCCCTGTT CACAACATGCTGCCAGGGCACCAGCGGCTGTCGGTGACGAGCCTGCTCGTCTGCCACGGATTGCTGATG GTCGGCACCAGCCTGGGAGTCCTCGTGGCCCTGCCGGTCCCACGTCTGCAAGGGATTCCCAAAGTGACC GGAAGAGGCATGGTCTCCTACCATGCACACAACAGTCCTGTCAAATTCATCGTCCTGGCCACGGCTCTG CACGAGAAAGACAAGGACAAATCCAGGGACAGCCTGGCTCCTGGCCCCGAGCCTCAGGACGAAGACCAG AAGGACGCACTTCCGAGTGGAGGAGCTGGTTCATCTCTGAGCCAGGGTGACCCTGACGCAGCCATCTGG TTGGGAGATTCGCTGGGATCGATGACTCAGAAAAGCGACCTGTCCTCCTCATCTGGGTCCCTGAGCTTG TCTCACGGCTCCAGCTCTCTAGAGCACAGATCAGAGGACAGCACCATCTATGATCTCCTGAAGGATCCT GTCTCGCTGAGAAGCAAAGCACGCCGGGCCAAGAAAGCCAAGGCCAGCTCGGCGCTGGTGGTCTGTGGA GGGCAGGGCCACCGCCGGGTGCACAGGAAGGCCCGGCAGCCCCACCAGGAAGAGCTGGCGCCGACCGTC ATGGTCTGGCAGATCCCTCTGCTGAATATATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_014629 |
| Insert Size | 4035 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014629.3 |
| RefSeq Size | 5602 bp |
| RefSeq ORF | 4035 bp |
| Locus ID | 9639 |
| UniProt ID | O15013 |
| Domains | RhoGEF |
| Protein Families | Druggable Genome |
| MW | 148.9 kDa |
| Gene Summary | This gene encodes a Rho guanine nucleotide exchange factor (GEF). Rho GEFs regulate the activity of small Rho GTPases by stimulating the exchange of guanine diphosphate (GDP) for guanine triphosphate (GTP) and may play a role in neural morphogenesis. Mutations in this gene are associated with slowed nerve conduction velocity (SNCV). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] Transcript Variant: This variant (1) encodes isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221903 | ARHGEF10 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 10 (ARHGEF10) |
CNY 9200.00 |
|
| RC221903L3 | Lenti-ORF clone of ARHGEF10 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 10 (ARHGEF10) |
CNY 15300.00 |
|
| RC221903L4 | Lenti-ORF clone of ARHGEF10 (mGFP-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 10 (ARHGEF10) |
CNY 15300.00 |
|
| RG221903 | ARHGEF10 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 10 (ARHGEF10) |
CNY 14730.00 |
|
| SC114960 | ARHGEF10 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 10 (ARHGEF10) |
CNY 12536.00 |
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