RC3H2 (NM_018835) Human Untagged Clone
CAT＃: SC318475
RC3H2 (untagged)-Human ring finger and CCCH-type domains 2 (RC3H2), transcript variant 2
CNY 20520.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MNAB; RNF164 |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_018835, the custom clone sequence may differ by one or more nucleotides
ATGCCTGTGCAGGCAGCTCAATGGACAGAATTTCTGTCCTGTCCAATCTGCTATAATGAA TTTGATGAGAATGTGCACAAACCCATCAGTTTAGGTTGTTCACACACTGTTTGCAAGACC TGCTTGAATAAACTTCATCGAAAAGCTTGTCCTTTTGACCAGACTGCCATCAACACAGAT ATTGATGTACTTCCTGTCAACTTCGCACTTCTCCAGTTAGTTGGAGCCCAGGTACCAGAT CATCAGTCAATTAAGTTAAGTAATCTAGGTGAGAATAAACACTATGAGGTTGCAAAGAAA TGCGTTGAGGATTTGGCACTCTACTTAAAACCACTAAGTGGAGGTAAAGGTGTAGCTAGC TTGAACCAGAGTGCACTGAGCCGTCCAATGCAAAGGAAACTGGTGACACTTGTAAACTGT CAACTGGTGGAGGAAGAAGGTCGTGTAAGAGCCATGCGAGCAGCTCGTTCCCTTGGAGAA AGAACTGTAACAGAACTGATATTACAGCACCAGAACCCTCAGCAGTTGTCTGCCAATCTA TGGGCCGCTGTCAGGGCTCGAGGATGCCAGTTTTTAGGGCCAGCTATGCAAGAAGAGGCC TTGAAGCTGGTGTTACTGGCATTAGAAGATGGTTCTGCCCTCTCAAGGAAAGTTCTGGTA CTTTTTGTTGTGCAGAGACTAGAACCAAGATTTCCTCAGGCATCAAAAACAAGTATTGGT CATGTTGTGCAACTACTGTATCGAGCTTCTTGTTTTAAGGTTACCAAAAGAGATGAAGAC TCTTCCCTAATGCAGCTGAAGGAGGAATTTCGGAGTTATGAAGCATTACGCAGAGAACAT GATGCCCAAATTGTTCATATTGCCATGGAAGCAGGACTCCGTATTTCACCTGAACAGTGG TCCTCTCTTTTGTATGGTGATTTGGCTCATAAATCACACATGCAGTCTATCATTGATAAG CTACAGTCTCCAGAGTCATTTGCAAAGAGTGTCCAGGAATTGACAATTGTTTTGCAACGA ACAGGTGACCCAGCTAACTTAAATAGACTGAGGCCTCATTTAGAGCTTCTTGCAAACATA GACCCTAATCCAGACGCTGTTTCACCAACTTGGGAGCAGCTGGAAAATGCAATGGTAGCT GTTAAAACAGTAGTTCATGGCCTTGTGGACTTCATACAAAATTATAGTAGAAAAGGCCAT GAGACCCCTCAGCCTCAGCCAAACAGCAAATACAAGACTAGCATGTGCCGAGATTTGCGA CAGCAAGGGGGTTGTCCACGAGGAACAAATTGTACATTTGCCCATTCTCAGGAAGAGCTT GAAAAGTATCGATTAAGGAACAAAAAGATCAATGCCACTGTAAGAACGTTTCCTCTTCTA AATAAAGTTGGTGTAAACAACACTGTCACAACCACAGCCGGAAATGTCATTTCTGTCATA GGAAGTACTGAAACAACAGGGAAAATTGTTCCAAGTACAAACGGAATTTCAAATGCAGAA AACAGTGTTTCCCAGCTAATCTCACGTAGTACTGACAGTACCTTAAGAGCTCTGGAGACC GTGAAGAAAGTGGGAAAGGTTGGCGCTAATGGTCAGAATGCTGCTGGGCCCTCTGCAGAT TCTGTAACTGAAAATAAAATTGGTTCTCCACCCAAGACTCCTGTAAGTAATGTAGCAGCT ACCTCAGCTGGGCCCTCTAATGTTGGAACAGAGCTGAATTCTGTGCCTCAAAAATCCAGC CCATTTCTAACTAGAGTACCAGTATATCCTCCGCATTCTGAAAACATTCAGTATTTTCAA GATCCAAGGACTCAGATACCCTTTGAAGTCCCACAGTACCCACAGACAGGATACTATCCA CCACCTCCAACGGTACCAGCTGGTGTGGCTCCCTGTGTTCCTCGCTTTGTGAGGTCCAAT AACGTTCCAGAGTCCTCCCTCCCACCTGCTTCCATGCCATATGCCGATCATTACAGTACA TTTTCCCCTCGAGATCGAATGAATTCTTCTCCTTACCAGCCTCCTCCTCCGCAGCCGTAT GGACCAGTTCCTCCAGTACCTTCTGGAATGTATGCTCCTGTGTACGACAGCAGGCGCATC TGGCGCCCACCTATGTACCAACGAGATGACATTATTAGAAGCAATTCTTTACCTCCAATG GATGTGATGCACTCATCTGTCTATCAGACATCTTTGCGGGAAAGATATAACTCATTAGAT GGATATTATTCGGTGGCTTGTCAGCCACCAAGTGAGCCAAGGACAACTGTGCCTTTACCA AGGGAACCTTGTGGTCATTTGAAGACCAGTTGCGAGGAGCAGATAAGAAGAAAGCCAGAT CAGTGGGCACAGTACCACACTCAGAAAGCACCTCTTGTCTCTTCAACTCTTCCTGTGGCA ACACAGTCACCAACACCACCTTCTCCTCTGTTCAGTGTAGACTTTCGTGCGGATTTCTCA GAGAGTGTGAGTGGTACAAAATTTGAAGAAGATCATCTTTCCCATTATTCTCCCTGGTCT TGTGGCACCATAGGCTCCTGTATAAATGCCATTGATTCAGAGCCCAAAGATGTCATTGCT AATTCAAATGCTGTGTTAATGGACCTGGACAGTGGTGATGTTAAGAGAAGAGTACATTTA TTTGAAACCCAGAGAAGGACAAAAGAAGAAGATCCAATAATTCCCTTTAGTGATGGACCC ATCATCTCAAAATGGGGTGCGATTTCCAGATCTTCCCGTACAGGTTACCATACCACAGAT CCTGTCCAGGCCACTGCTTCCCAAGGAAGTGCGACTAAGCCCATCAGTGTATCAGATTAT GTCCCTTATGTCAATGCTGTTGATTCAAGGTGGAGTTCATATGGCAACGAGGCCACATCA TCAGCACACTATGTTGAAAGGGACAGATTCATTGTTACTGATTTATCTGGTCATAGAAAG CATTCCAGTACTGGGGACCTTTTGAGCCTTGAACTTCAGCAGGCCAAGAGCAACTCATTA CTTCTTCAGAGAGAGGCCAATGCTTTGGCCATGCAACAGAAGTGGAATTCCCTGGATGAA GGCCGTCACCTTACCTTAAACCTTTTAAGCAAGGAAATTGAACTAAGAAATGGAGAGGTA AAGAAACTGAACCTTTCGGCTTCATGCTTAATGTATTTGTTTTCTGCTGCTGCATCTTGG TTATACCATTAC |
| Restriction Sites | Please inquire |
| ACCN | NM_018835 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018835.2, NP_061323.2 |
| RefSeq Size | 3581 bp |
| RefSeq ORF | 3195 bp |
| Locus ID | 54542 |
| UniProt ID | Q9HBD1 |
| Domains | zf-CCCH |
| Protein Families | Druggable Genome |
| Gene Summary | Post-transcriptional repressor of mRNAs containing a conserved stem loop motif, called constitutive decay element (CDE), which is often located in the 3' UTR, as in HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF and in many more mRNAs. Binds to CDE and promotes mRNA deadenylation and degradation. This process does not involve miRNAs. In follicular helper T (Tfh) cells, represses of ICOS and TNFRSF4 expression, thus preventing spontaneous Tfh cell differentiation, germinal center B-cell differentiation in the absence of immunization and autoimmunity. In resting or LPS-stimulated macrophages, controls inflammation by suppressing TNF expression. Also recognizes CDE in its own mRNA and in that of paralogous RC3H1, possibly leading to feedback loop regulation (By similarity). miRNA-binding protein that regulates microRNA homeostasis. Enhances DICER-mediated processing of pre-MIR146a but reduces mature MIR146a levels through an increase of 3' end uridylation. Both inhibits ICOS mRNA expression and they may act together to exert the suppression (PubMed:25697406). Acts as a ubiquitin E3 ligase. Pairs with E2 enzymes UBE2B, UBE2D2, UBE2E2, UBE2E3, UBE2G2, UBE2K and UBE2Q2 and produces polyubiquitin chains (PubMed:26489670). Show the strongest activity when paired with UBE2N:UBE2V1 or UBE2N:UBE2V2 E2 complexes and generate both short and long polyubiquitin chains (PubMed:26489670). Involved in the ubiquitination of MAP3K5 (PubMed:24448648, PubMed:26489670) (By similarity). Able to interact with double-stranded RNA (dsRNA) (PubMed:26489670).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has multiple differences in the presence and absence of exons at its 3' end, compared to variant 1. These differences produce a unique 3' UTR, and a protein (isoform 2) that has a shorter and distinct C-terminus, compa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211760 | RC3H2 (Myc-DDK-tagged)-Human ring finger and CCCH-type domains 2 (RC3H2), transcript variant 2 |
CNY 10640.00 |
|
| RC211760L3 | Lenti-ORF clone of RC3H2 (Myc-DDK-tagged)-Human ring finger and CCCH-type domains 2 (RC3H2), transcript variant 2 |
CNY 12540.00 |
|
| RC211760L4 | Lenti-ORF clone of RC3H2 (mGFP-tagged)-Human ring finger and CCCH-type domains 2 (RC3H2), transcript variant 2 |
CNY 12540.00 |
|
| RG211760 | RC3H2 (tGFP-tagged) - Human ring finger and CCCH-type domains 2 (RC3H2), transcript variant 2 |
CNY 11690.00 |
