EML4 (NM_019063) Human Untagged Clone
CAT＃: SC318431
EML4 (untagged)-Human echinoderm microtubule associated protein like 4 (EML4), transcript variant 1
CNY 18704.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C2orf2; ELP120; EMAP-4; EMAPL4; ROPP120 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_019063 edited
ATGGACGGTTTCGCCGGCAGTCTCGATGATAGTATTTCTGCTGCAAGTACTTCTGATGTT CAAGATCGCCTGTCAGCTCTTGAGTCACGAGTTCAGCAACAAGAAGATGAAATCACTGTG CTAAAGGCGGCTTTGGCTGATGTTTTGAGGCGTCTTGCAATCTCTGAAGATCATGTGGCC TCAGTG:AAAAAATCAGTCTCAAGTAAAGGCCAACCAAGCCCTCGAGCAGTTATTCCCAT GTCCTGTATAACCAATGGAAGTGGTGCAAACAGAAAACCAAGTCATACCAGTGCTGTCTC AATTGCAGGAAAAGAAACTCTTTCATCTGCTGCTAAAAGTGGTACAGAAAAAAAGAAAGA AAAACCACAAGGACAGAGAGAAAAAAAAGAGGAATCTCATTCTAATGATCAAAGTCCACA AATTCGAGCATCACCTTCTCCCCAGCCCTCTTCACAACCTCTCCAAATACACAGACAAAC TCCAGAAAGCAAGAATGCTACTCCCACCAAAAGCATAAAACGACCATCACCAGCTGAAAA GTCACATAATTCTTGGGAAAATTCAGATGATAGCCGTAATAAATTGTCGAAAATACCTTC AACACCCAAATTAATACCAAAAGTTACCAAAACTGCAGACAAGCATAAAGATGTCATCAT CAACCAAGAAGGAGAATATATTAAAATGTTTATGCGCGGTCGGCCAATTACCATGTTCAT TCCTTCCGATGTTGACAACTATGATGACATCAGAACGGAACTGCCTCCTGAGAAGCTCAA ACTGGAGTGGGCATATGGTTATCGAGGAAAGGACTGTAGAGCTAATGTTTACCTTCTTCC GACCGGGGAAATAGTTTATTTCATTGCATCAGTAGTAGTACTATTTAATTATGAGGAGAG AACTCAGCGACACTACCTGGGCCATACAGACTGTGTGAAATGCCTTGCTATACATCCTGA CAAAATTAGGATTGCAACTGGACAGATAGCTGGCGTGGATAAAGATGGAAGGCCTCTACA ACCCCACGTCAGAGTGTGGGATTCTGTTACTCTATCCACACTGCAGATTATTGGACTTGG CACTTTTGAGCGTGGAGTAGGATGCCTGGATTTTTCAAAAGCAGATTCAGGTGTTCATTT ATGTGTTATTGATGACTCCAATGAGCATATGCTTACTGTATGGGACTGGCAGAGGAAAGC AAAAGGAGCAGAAATAAAGACAACAAATGAAGTTGTTTTGGCTGTGGAGTTTCACCCAAC AGATGCAAATACCATAATTACATGCGGTAAATCTCATATTTTCTTCTGGACCTGGAGCGG CAATTCACTAACAAGAAAACAGGGAATTTTTGGGAAATATGAAAAGCCAAAATTTGTGCA GTGTTTAGCATTCTTGGGGAATGGAGATGTTCTTACTGGAGACTCAGGTGGAGTCATGCT TATATGGAGCAAAACTACTGTAGAGCCCACACCTGGGAAAGGACCTAAAGGTGTATATCA AATCAGCAAACAAATCAAAGCTCATGATGGCAGTGTGTTCACACTTTGTCAGATGAGAAA TGGGATGTTATTAACTGGAGGAGGGAAAGACAGAAAAATAATTCTGTGGGATCATGATCT GAATCCTGAAAGAGAAATAGAGGTTCCTGATCAGTATGGCACAATCAGAGCTGTAGCAGA AGGAAAGGCAGATCAATTTTTAGTAGGCACATCACGAAACTTTATTTTACGAGGAACATT TAATGATGGCTTCCAAATAGAAGTACAGGGTCATACAGATGAGCTTTGGGGTCTTGCCAC ACATCCCTTCAAAGATTTGCTCTTGACATGTGCTCAGGACAGGCAGGTGTGCCTGTGGAA CTCAATGGAACACAGGCTGGAATGGACCAGGCTGGTAGATGAACCAGGACACTGTGCAGA TTTTCATCCAAGTGGCACAGTGGTGGCCATAGGAACGCACTCAGGCAGGTGGTTTGTTCT GGATGCAGAAACCAGAGATCTAGTTTCTATCCACACAGACGGGAATGAACAGCTCTCTGT GATGCGCTACTCAATAGATGGTACCTTCCTGGCTGTAGGATCTCATGACAACTTTATTTA CCTCTATGTAGTCTCTGAAAATGGAAGAAAATATAGCAGATATGGAAGGTGCACTGGACA TTCCAGCTACATCACACACCTTGACTGGTCCCCAGACAACAAGTATATAATGTCTAACTC GGGAGACTATGAAATATTGTACTGGGACATTCCAAATGGCTGCAAACTAATCAGGAATCG ATCGGATTGTAAGGACATTGATTGGACGACATATACCTGTGTGCTAGGATTTCAAGTATT TGGTGTCTGGCCAGAAGGATCTGATGGGACAGATATCAATGCACTGGTGCGATCCCACAA TAGAAAGGTGATAGCTGTTGCCGATGACTTTTGTAAAGTCCATCTGTTTCAGTATCCCTG CTCCAAAGCAAAGGCTCCCAGTCACAAGTACAGTGCCCACAGCAGCCATGTCACCAATGT CAGTTTTACTCACAATGACAGTCACCTGATATCAACTGGTGGAAAAGACATGAGCATCAT TCAGTGGAAACTTGTGGAAAAGTTATCTTTGCCTCAGAATGAGACTGTAGCGGATACTAC TCTAACCAAAGCCCCCGTCTCTTCCACTGAAAGTGTCATCCAATCTAATACTCCCACACC GCCTCCTTCTCAGCCCTTAAATGAGACAGCTGAAGAGGAAAGTAGAATAAGCAGTTCTCC CACACTTCTGGAGAACAGCCTGGAACAAACTGTGGAGCCAAGTGAAGACCACAGCGAGGA GGAGAGTGAAGAGGGCAGCGGAGACCTTGGTGAGCCTCTTTATGAAGAGCCATGCAACGA GATAAGCAAGGAGCAGGCCAAAGCCACCCTTCTGGAGGACCAGCAAGACCCTTCGCCCTC GTCCTAA |
| Restriction Sites | Please inquire |
| ACCN | NM_019063 |
| Insert Size | 5577 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019063.2, NP_061936.2 |
| RefSeq Size | 5577 bp |
| RefSeq ORF | 5565 bp |
| Locus ID | 27436 |
| UniProt ID | Q9HC35 |
| Domains | WD40, HELP |
| Protein Families | Druggable Genome |
| Gene Summary | This gene is a member of the echinoderm microtubule associated protein-like family. The encoded WD-repeat protein may be involved in microtubule formation. Abnormal fusion of parts of this gene with portions of the anaplastic lymphoma receptor tyrosine kinase gene, which generates EML4-ALK fusion transcripts, is one of the primary mutations associated with non-small cell lung cancer. Alternative splicing of this gene results in two transcript variants. [provided by RefSeq, Jan 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214653 | EML4 (Myc-DDK-tagged)-Human echinoderm microtubule associated protein like 4 (EML4), transcript variant 1 |
CNY 11496.00 |
|
| RC214653L1 | Lenti-ORF clone of EML4 (Myc-DDK-tagged)-Human echinoderm microtubule associated protein like 4 (EML4), transcript variant 1 |
CNY 13896.00 |
|
| RC214653L2 | Lenti-ORF clone of EML4 (mGFP-tagged)-Human echinoderm microtubule associated protein like 4 (EML4), transcript variant 1 |
CNY 5890.00 |
|
| RC214653L3 | Lenti-ORF clone of EML4 (Myc-DDK-tagged)-Human echinoderm microtubule associated protein like 4 (EML4), transcript variant 1 |
CNY 5890.00 |
|
| RC214653L4 | Lenti-ORF clone of EML4 (mGFP-tagged)-Human echinoderm microtubule associated protein like 4 (EML4), transcript variant 1 |
CNY 5890.00 |
|
| RG214653 | EML4 (tGFP-tagged) - Human echinoderm microtubule associated protein like 4 (EML4), transcript variant 1 |
CNY 4370.00 |
