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PIF1 (NM_025049) Human Untagged Clone
CAT＃: SC318097
PIF1 (untagged)-Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1)
CNY 7,168.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C15orf20; PIF |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_025049 edited
TGAGTGTCGGCACAAACCAGCAGAGGCGGTGACGATGCTCTCGGGCATAGAGGCGGCGGC AGGGGAATATGAGGACTCGGAGCTGCGGTGCCGCGTGGCTGTGGAGGAGCTGAGCCCGGG CGGGCAGCCGCGAAGGCGCCAGGCCCTGCGCACCGCGGAGCTGAGCCTGGGTCGCAACGA GCGCCGCGAGTTGATGCTGCGGCTGCAAGCGCCAGGGCCCGCGGGGCGGCCGCGCTGCTT CCCTCTGCGCGCCGCGCGCCTCTTCACGCGTTTCGCCGAGGCCGGGCGCAGCACCCTGCG GCTCCCCGCCCACGACACCCCCGGGGCCGGCGCAGTGCAGCTGCTGCTCTCGGACTGCCC CCCAGACCGCCTGCGCCGCTTCCTGCGCACATTGCGCCTCAAGCTGGCTGCGGCCCCGGG TCCCGGGCCGGCCTCCGCCCGAGCGCAGCTGCTGGGCCCGCGGCCCCGCGACTTCGTCAC CATCAGCCCTGTGCAGCCCGAGGAGCGGCGGCTCAGGGCGGCCACCCGGGTTCCGGACAC TACGCTGGTGAAGCGGCCTGTGGAGCCCCAGGCTGGGGCCGAGCCTAGCACAGAAGCCCC AAGGTGGCCCCTGCCTGTGAAGAGGCTGAGCTTGCCCTCCACCAAGCCACAGCTTTCTGA GGAACAGGCTGCTGTGCTGAGGGCCGTCCTGAAAGGCCAGAGCATCTTCTTCACTGGGAG TGCAGGAACAGGGAAGTCATATCTGCTAAAGCGAATCCTGGGCTCACTGCCCCCCACAGG CACTGTGGCCACTGCCAGCACTGGGGTGGCAGCCTGCCACATCGGGGGCACCACCCTCCA TGCCTTTGCAGGCATCGGCTCAGGCCAGGCTCCTCTAGCCCAGTGTGTGGCCCTGGCCCA AAGGCCAGGCGTGCGGCAGGGCTGGCTGAACTGCCAGCGGTTGGTCATTGACGAGATCTC AATGGTGGAGGCAGACCTGTTTGACAAACTGGAGGCCGTGGCCAGAGCTGTCCGGCAGCA GAACAAGCCATTCGGAGGGATCCAGCTCATCATCTGTGGGGACTTTCTGCAGCTGCCACC TGTGACCAAGGGCTCCCAGCCCCCACGGTTCTGCTTCCAGTCCAAGAGCTGGAAGAGGTG TGTGCCAGTGACCCTGGAGCTGACCAAGGTGTGGAGGCAGGCAGACCAGACCTTCATCTC TCTACTGCAGGCCGTGAGGCTAGGCAGGTGTTCAGATGAGGTGACCCGCCAGCTCCAGGC CACAGCTTCCCACAAGGTGGGGCGAGATGGGATTGTGGCCACGAGGCTCTGCACCCACCA GGATGATGTGGCCCTCACCAACGAGAGGCGGCTTCAGGAGCTGCCAGGTAAGGTACACAG ATTTGAGGCTATGGACAGCAACCCTGAGCTGGCCAGTACCCTGGATGCCCAGTGTCCTGT TAGCCAGCTCCTTCAACTAAAGCTGGGGGCCCAGGTGATGCTGGTGAAAAACTTATCGGT GTCTCGGGGCCTGGTGAATGGTGCCCGAGGGGTGGTAGTTGGGTTCGAGGCAGAAGGGAG AGGGCTACCCCAGGTGCGGTTCCTGTGTGGAGTCACTGAGGTCATCCACGCTGACCGCTG GACGGTGCAGGCCACCGGGGGCCAGCTCCTCAGTCGGCAGCAGCTGCCCCTCCAGCTGGC CTGGGCGATGTCCATCCACAAGAGCCAAGGCATGACCCTGGATTGTGTGGAGATTTCTCT GGGCCGTGTGTTTGCCAGTGGCCAGGCCTATGTGGCCCTTTCTCGGGCCCGCAGCCTGCA GGGCCTACGTGTGCTGGACTTTGACCCCATGGCGGTTCGCTGTGACCCCCGTGTGCTGCA CTTCTATGCCACCCTGCGGCGGGGCAGGAGCCTCAGTCTGGAGTCCCCAGATGATGATGA GGCAGCCTCAGACCAGGAGAACATGGACCCAATCCTCTGAGCCTCACCCACAAAGAGAAG ACAAAGGGTGGCCTGTGGCCTCCCCGTCCTCCTGCTCCCTAGTGGCCCAGGGCCTCAGGG AATAACTGGAGTAGGCAGGCAGTGTCCCCTTCTGTATTTTTTAGGGACTCTCAGCCTCCT GGCAGGGTTAAAGGGAGAGTACTTTAAACCCATATACCAGCTGTGCTTCAGTTTCTCTCA CTTTGCCCTGGGTAAGCTGCTGTAGGGTCAGAAGTAACCCTTTCTGTGCCAGTTGAGAAT GAGCCTGTGTGGTAGCTGATGTCAGAGGACAAAGCTCTCTGCAAGGGCTGGACACAGAGC TGCAGAGTCCTGAACATCCCTCCTTTCAGGCTGCAGAAGGGAGAGGCAATGAAGACAGGT GCTCCGGAAGCAGCATCAGGGCTCTTGGAGGGGACTGGTGGGGACTCAGGCTGGGTGCAG CCTCCAAACAGAGAACGGAACTTAGGTGTGTCTCTACAGCTAGGCCCAGCCTAGCCCAGC CCAGAACAAACACCCTTCAGAGCCTAACCAAAGAACATAAGCTGCAAAATGTGCACCCAT ATTTTAAGCTGCTTTTTCAGGGGATAAATAGTGTTTGGGGACATTGAAATGGATGTTCTC AGGTTGTATTTATTTCGGACAAATAAACTAGAGAATTGTGTAAAAAAAAAAAAAAAAAAA AAA >OriGene 5' read for NM_025049 unedited
CCTCATTCCCCCGCCCGTTGNCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATAT AAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGC CGCGAATTCGGCACGAGGTGAGTGTCGGCACAAACCAGCAGAGGCGGTGACGATGCTCTC GGGCATAGAGGCGGCGGCAGGGGAATATGAGGACTCGGAGCTGCGGTGCCGCGTGGCTGT GGAGGAGCTGAGCCCGGGCGGGCAGCCGCGAAGGCGCCAGGCCCTGCGCACCGCGGAGCT GAGCCTGGGTCGCAACGAGCGCCGCGAGTTGATGCTGCGGCTGCAAGCGCCAGGGCCCGC GGGGCGGCCGCGCTGCTTCCCTCTGCGCGCCGCGCGCCTCTTCACGCGTTTCGCCGAGGC CGGGCGCAGCACCCTGCGGCTCCCCGCCCACGACACCCCCGGGGCCGGCGCAGTGCAGCT GCTGCTCTCGGACTGCCCCCCAGACCGCCTGCGCCGCTTCCTGCGCACATTGCGCCTCAA GCTGGCTGCGGCCCCGGGTCCCGGGCCGGCCTCCGCCCGAGCGCAGCTGCTGGGCCCGCG GCCCCGCGACTTCGTCACCATCAGCCCTGTGCAGCCCGAGGAGCGGCGGCTCAGGGCGGC CACCCGGGTTCCGGACACTACGCTGGTGAAGCGGCCTGTGGAGCCCCACGCTGGGGCCGC GCCTAGCACAGAAGCCCTAGGTGGCCCCTGCCTGTGAAGAGGCTGCACTTGCCCTCCACC AGCCCAGTTTCTGAGCACAGGCTGTGGTCTGGGTCCTCCTGACAGGCCATACATTTCTTC ATGGTCTGACGAACCGGGAATCCTATTTGTTCAAGCGATCCTGGTCTCCTGTCCCCCCGG CCTG |
| Restriction Sites | Please inquire |
| ACCN | NM_025049 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025049.2, NP_079325.2 |
| RefSeq Size | 2659 bp |
| RefSeq ORF | 1926 bp |
| Locus ID | 80119 |
| UniProt ID | Q9H611 |
| Gene Summary | This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus. [provided by RefSeq, Nov 2013] Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. This variant can initiate translation from both an upstream and a downstream AUG start codon. The isoform represented in this variant (a, also known as alpha or alpha-1) initiates translation from the upstream AUG. This isoform localizes to the nucleus (PMID: 17827721). Variants 1 and 2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223376 | PIF1 (Myc-DDK-tagged)-Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1) |
CNY 7,160.00 |
|
| RC223376L1 | Lenti ORF clone of Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1), Myc-DDK-tagged |
CNY 9,560.00 |
|
| RC223376L2 | Lenti ORF clone of Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1), mGFP tagged |
CNY 9,560.00 |
|
| RC223376L3 | Lenti ORF clone of Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC223376L4 | Lenti ORF clone of Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1), mGFP tagged |
CNY 9,560.00 |
|
| RG223376 | PIF1 (tGFP-tagged) - Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1) |
CNY 8,760.00 |
|
| SC312706 | PIF1 (untagged)-Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1) |
CNY 3,600.00 |
