PDZD3 (NM_024791) Human Untagged Clone
CAT＃: SC317823
PDZD3 (untagged)-Human PDZ domain containing 3 (PDZD3), transcript variant 2
CNY 7890.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | IKEPP; NHERF4; PDZK2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC317823 representing NM_024791.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGAAAGCCGCAGATCTGCAGGACACAGCCTCGTTAACTCTGAAGTTTAAGTTTAACCCAAAGCTG GGCATTGATAATCCTGTCCTCTCCCTGGCCGAAGACCACGACCCCTATGATCCCTGGAGCCTGGAGCGG CCTCGCTTCTGTTTACTGAGCAAAGAGGAGGGCAAGAGTTTTGGCTTCCACCTGCAGCAGGAGCTGGGC AGGGCTGGGCATGTGGTGTGCAGGGTGGACCCAGGCACCTCTGCCCAGCGCCAGGGTCTTCAGGAAGGA GACAGGATCCTGGCGGTGAACAATGATGTTGTGGAACACGAAGACTATGCGGTGGTGGTACGCCGCATC CGGGCCAGCAGCCCTCGGGTGTTGCTGACAGTATTGGCACGGCATGCACATGACGTGGCCCGAGCTCAG CTGGGAGAAGATGCCCACCTCTGTCCCACCCTAGGCCCAGGGGTCCGGCCCCGGCTGTGCCACATAGTG AAAGATGAGGGTGGTTTTGGCTTCAGTGTCACCCATGGCAATCAGGGTCCTTTCTGGTTGGTGCTAAGT ACTGGAGGAGCAGCTGAGCGGGCAGGGGTGCCCCCCGGGGCCCGGCTGCTGGAAGTGAATGGGCTTTGG CAGAGTGGACAGCAGGTGACCTTGCTGGTGGCAGGGCCAGAGGTGGAAGAACAGTGTCGCCAGCTGGGA TTGCCCCTGGCTGCACCCCTGGCAGAGGGCTGGGCACTGCCCACCAAGCCCCGCTGCCTGCACCTGGAG AAAGGGCCCCAGGGTTTTGGGTTCCTGCTCCGGGAGGAAAAGGGCCTTGACGGTCGCCCTGGACAGTTC CTGTGGGAGGTGGACCCGGGACTGCCAGCCAAGAAGGCTGGGATGCAGGCTGGGGACCGGCTGGTGGCT GTGGCTGGGGAGAGCGTGGAGGGGCTGGGCCATGAGGAGACAGTGTCCAGGATCCAGGGGCAGGGCTCC TGTGTCTCCCTCACTGTCGTCGACCCTGAGGCGGACCGCTTCTTCAGCATGGTTCGCCTGTCCCCACTC CTCTTCTTGGAGAACACAGAGGCTCCCGCCTCGCCCCGGGGCAGCAGCTCAGCCTCACTGGTTGAGACA GAGGACCCTTCACTTGAAGACACAAGCGTGCCTTCTGTCCCTCTTGGCTCCCGACAGTGCTTCCTGTAC CCTGGGCCTGGTGGCAGCTATGGCTTCCGACTCAGTTGTGTGGCCAGTGGGCCTCGTCTCTTCATCTCC CAGGTGACTCCAGGAGGCTCAGCTGCCCGGGCTGGGCTGCAAGTGGGAGACGTGATTCTGGAAGTGAAC GGGTATCCTGTTGGGGGACAGAATGACCTGGAGAGGCTTCAGCAGCTGCCTGAGGCTGAGCCACCCCTC TGCCTGAAGCTGGCAGCCAGGTCTCTGCGGGGCTTGGAAGCCTGGATTCCCCCTGGGGCTGCAGAGGAC TGGGCTCTGGCCTCGGATCTACTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_024791 |
| Insert Size | 1476 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024791.3 |
| RefSeq Size | 2245 bp |
| RefSeq ORF | 1476 bp |
| Locus ID | 79849 |
| UniProt ID | Q86UT5 |
| Protein Families | Druggable Genome |
| MW | 52.5 kDa |
| Gene Summary | Guanylyl cyclase C (GCC, or GUCY2C; MIM 601330) produces cGMP following the binding of either endogenous ligands or heat-stable enterotoxins secreted by E. coli and other enteric bacteria. Activation of GCC initiates a signaling cascade that leads to phosphorylation of the cystic fibrosis transmembrane conductance regulator (CFTR; MIM 602421), followed by a net efflux of ions and water into the intestinal lumen. IKEPP is a regulatory protein that associates with GCC and regulates the amount of cGMP produced following receptor stimulation (Scott et al., 2002 [PubMed 11950846]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206415 | PDZD3 (Myc-DDK-tagged)-Human PDZ domain containing 3 (PDZD3), transcript variant 2 |
CNY 3656.00 |
|
| RC206415L1 | Lenti ORF clone of Human PDZ domain containing 3 (PDZD3), transcript variant 2, Myc-DDK-tagged |
CNY 6056.00 |
|
| RC206415L2 | Lenti ORF clone of Human PDZ domain containing 3 (PDZD3), transcript variant 2, mGFP tagged |
CNY 6180.00 |
|
| RC206415L3 | Lenti ORF clone of Human PDZ domain containing 3 (PDZD3), transcript variant 2, Myc-DDK-tagged |
CNY 6180.00 |
|
| RC206415L4 | Lenti ORF clone of Human PDZ domain containing 3 (PDZD3), transcript variant 2, mGFP tagged |
CNY 6180.00 |
|
| RG206415 | PDZD3 (tGFP-tagged) - Human PDZ domain containing 3 (PDZD3), transcript variant 2 |
CNY 5256.00 |
|
| SC122970 | PDZD3 (untagged)-Human PDZ domain containing 3 (PDZD3), transcript variant 2 |
CNY 2400.00 |
