PRAGMIN (PRAG1) (NM_001080826) Human Untagged Clone
CAT＃: SC317167
SGK223 (untagged)-Human homolog of rat pragma of Rnd2 (SGK223)
CNY 27080.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NACK; PEAK2; PRAGMIN; SGK223 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC317167 representing NM_001080826.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCACCAGACCCTCTGCCTGAACCCCGAGAGCCTGAAAATGTCTGCGTGCAGTGACTTTGTGGAGCAC ATCTGGAAACCCGGGTCCTGCAAGAACTGCTTCTGCCTGCGGAGCGACCACCAGCTGGTGGCCGGCCCT CCCCAGCCCAGAGCGGGCAGCCTGCCCCCTCCACCGCGCCTGCCTCCCAGGCCTGAGAACTGCCGCCTG GAAGATGAAGGTGTGAACAGCTCACCTTACTCCAAGCCCACAATTGCCGTGAAGCCCACCATGATGAGC TCCGAGGCCTCTGATGTGTGGACAGAGGCCAACCTGAGTGCCGAAGTCTCGCAGGTCATCTGGAGACGA GCCCCTGGCAAGCTCCCCCTCCCGAAGCAGGAGGATGCCCCCGTCGTCTACCTGGGCAGCTTCCGAGGT GTACAGAAGCCTGCTGGTCCCTCTACCTCCCCTGATGGCAATTCTCGCTGTCCCCCAGCTTACACCATG GTCGGCCTGCACAACCTTGAGCCCCGCGGCGAGAGGAACATTGCCTTCCACCCGGTGAGCTTCCCGGAG GAGAAGGCTGTGCACAAAGAAAAACCCTCATTTCCTTACCAAGACCGGCCCTCCACCCAGGAGAGCTTC CGCCAGAAACTGGCTGCCTTTGCTGGGACCACATCTGGCTGTCACCAGGGCCCTGGGCCCCTGCGGGAA TCCCTGCCCTCGGAGGATGACAGTGATCAAAGGTGCTCGCCCTCCGGGGACAGCGAGGGTGGAGAGTAC TGCTCCATCCTGGACTGCTGCCCTGGGAGCCCTGTTGCCAAGGCTGCCTCCCAGACTGCAGGTTCCCGG GGCAGGCATGGTGGCAGGGACTGCTCACCCACGTGCTGGGAGCAGGGGAAGTGTTCCGGGCCCGCAGAG CAGGAGAAGCGGGGCCCGAGCTTCCCCAAGGAGTGCTGTAGCCAGGGCCCCACTGCCCACCCATCCTGC CTGGGCCCCAAGAAACTGTCCCTCACCTCGGAGGCTGCCATTTCTTCCGACGGCCTCTCTTGTGGCAGC GGCAGCGGCAGCGGCAGCGGCGCCAGTAGCCCCTTCGTCCCCCACCTCGAGAGTGATTACTGCTCCCTC ATGAAGGAACCTGCCCCAGAGAAGCAGCAGGACCCTGGCTGCCCAGGGGTGACCCCTAGCAGATGCCTT GGGCTGACGGGGGAGCCCCAGCCCCCGGCCCACCCCCGGGAGGCTACACAGCCTGAACCCATCTATGCT GAGAGCACCAAGAGGAAGAAGGCAGCTCCGGTGCCTTCCAAGTCACAGGCCAAGATAGAACATGCAGCT GCTGCCCAGGGCCAAGGCCAGGTATGCACAGGTAATGCCTGGGCCCAGAAAGCAGCATCTGGCTGGGGC CGGGACAGCCCAGACCCAACTCCCCAGGTGTCAGCCACCATCACAGTCATGGCGGCCCACCCGGAAGAG GACCATCGGACGATCTACCTGAGCAGCCCTGACTCTGCAGTGGGGGTGCAGTGGCCACGAGGGCCTGTG AGCCAGAACTCCGAGGTAGGTGAAGAGGAGACTTCGGCTGGGCAGGGGCTGAGCTCCAGGGAAAGCCAT GCTCACAGTGCCAGCGAGAGCAAGCCCAAGGAGAGGCCCGCCATTCCCCCCAAGTTGTCCAAGAGTAGC CCTGTAGGGTCCCCGGTGTCACCGTCTGCTGGAGGGCCCCCAGTGTCACCGCTGGCTGACCTTAGTGAT GGGAGCTCTGGCGGCAGCAGCATTGGGCCCCAGCCTCCATCCCAAGGTCCTGCTGACCCCGCTCCTTCC TGCCGGACCAACGGTGTCGCTATCAGTGACCCATCCAGGTGTCCCCAGCCTGCCGCCTCGTCAGCCTCG GAACAGAGGCGGCCCAGGTTCCAGGCAGGCACCTGGAGTCGTCAGTGCCGGATAGAGGAAGAAGAGGAG GTGGAGCAGGAATTGCTGAGTCACAGCTGGGGAAGAGAGACCAAAAATGGCCCCACGGACCATTCAAAC TCCACGACCTGGCACCGTCTCCACCCCACAGATGGCTCCTCTGGGCAGAACAGCAAAGTTGGGACCGGG ATGAGCAAATCCGCCTCTTTTGCCTTTGAGTTCCCCAAGGACAGAAGTGGGATTGAGACATTCTCACCT CCTCCTCCGCCTCCAAAGTCGCGGCACCTTCTAAAAATGAACAAGAGCAGCTCTGATTTGGAAAAAGTG AGCCAGGGCTCTGCAGAAAGCCTCAGCCCATCCTTCAGGGGTGTCCACGTCAGCTTCACCACCGGCTCC ACGGACAGCCTGGCCTCAGACTCTAGGACCTGCAGCGATGGAGGTCCCTCGTCTGAGCTGGCTCACTCG CCCACCAACAGCGGGAAGAAGCTCTTTGCTCCCGTTCCGTTTCCTTCAGGCTCCACTGAGGACGTGTCC CCCAGTGGCCCCCAGCAGCCCCCTCCACTCCCCCAGAAAAAGATAGTGAGCCGGGCAGCCTCTTCACCG GATGGCTTCTTCTGGACCCAAGGCTCCCCCAAGCCCGGAACAGCAAGCCCCAAGCTGAACCTAAGCCAC TCGGAAACCAACGTCCACGACGAATCTCACTTTAGCTATTCGTTGAGCCCCGGGAACCGCCACCATCCT GTCTTCTCCTCTTCCGATCCTCTGGAGAAAGCTTTCAAAGGCAGTGGCCACTGGCTTCCGGCAGCAGGG CTGGCGGGCAACAGAGGCGGCTGCGGGAGCCCTGGCCTCCAGTGCAAAGGGGCCCCCTCCGCCTCATCC TCCCAGCTGAGCGTGTCCAGTCAAGCCTCCACCGGGAGCACCCAGCTTCAGCTGCACGGTCTCCTGAGC AACATCAGCAGCAAGGAGGGCACCTATGCCAAGCTGGGGGGACTCTACACCCAGTCCCTGGCCCGCCTT GTAGCCAAATGTGAGGACCTCTTCATGGGCGGCCAGAAAAAGGAGCTCCACTTCAATGAGAATAACTGG TCGCTCTTCAAGCTGACTTGTAACAAGCCCTGCTGTGACTCGGGGGATGCCATTTATTACTGTGCCACC TGCTCTGAGGACCCCGGCAGCACCTATGCTGTGAAAATCTGCAAAGCCCCTGAGCCCAAAACAGTCTCC TACTGCAGCCCGTCCGTGCCCGTGCACTTTAACATCCAGCAGGACTGCGGCCACTTCGTCGCCTCGGTG CCGTCCAGCATGCTCAGCTCCCCCGACGCGCCCAAGGACCCTGTGCCTGCCCTGCCCACACACCCCCCT GCCCAGGAGCAGGACTGCGTGGTGGTCATCACCCGAGAGGTGCCACATCAGACCGCCTCCGACTTCGTG CGGGACTCGGCGGCCAGCCACCAGGCGGAGCCCGAGGCGTACGAGCGGCGCGTGTGCTTCCTGCTTCTG CAACTCTGCAACGGGCTGGAGCACCTGAAGGAGCACGGGATCATCCACCGGGACCTGTGCCTGGAGAAC CTGCTGCTGGTGCACTGCACCCTCCAGGCCGGCCCCGGGCCCGCCCCCGCCCCCGCCCCGGCTCCCGCC CCCGCCGCCGCCGCGCCTCCCTGCTCCTCTGCCGCCCCGCCTGCTGGTGGCACTCTCAGCCCCGCAGCC GGCCCCGCCTCCCCGGAAGGGCCCCGGGAGAAGCAGCTGCCCCGGCTCATCATCAGCAACTTTTTGAAG GCCAAGCAGAAGCCGGGCGGCACCCCAAACCTGCAGCAGAAGAAGAGCCAGGCCCGGCTGGCCCCCGAG ATCGTGTCTGCTTCCCAGTACCGCAAGTTCGATGAGTTCCAGACAGGCATCCTCATCTACGAGCTGCTG CACCAACCCAACCCGTTCGAGGTGCGCGCCCAGCTGCGGGAGAGAGACTACCGGCAGGAGGACCTGCCG CCGCTGCCCGCGCTGTCCCTCTACTCACCCGGCCTGCAGCAGCTGGCACATCTGCTACTGGAGGCCGAC CCCATCAAGCGTATCCGCATCGGCGAGGCCAAGCGCGTGCTGCAGTGCCTGCTGTGGGGGCCTCGGCGC GAGCTGGTGCAGCAGCCGGGCACCTCGGAGGAGGCGCTGTGCGGCACGCTGCACAACTGGATCGACATG AAGCGGGCCCTGATGATGATGAAGTTTGCGGAGAAGGCGGTGGATCGCAGGCGGGGCGTGGAGCTGGAG GACTGGCTTTGCTGCCAGTACCTGGCGTCTGCGGAGCCCGGGGCCCTCTTACAGTCGCTGAAGCTCCTG CAGCTTCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001080826 |
| Insert Size | 4221 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001080826.2 |
| RefSeq Size | 4726 bp |
| RefSeq ORF | 4221 bp |
| Locus ID | 157285 |
| UniProt ID | Q86YV5 |
| Protein Families | Protein Kinase |
| MW | 149.6 kDa |
| Gene Summary | This gene encodes an enzyme that belongs to the tyrosine protein kinase family. A similar protein in rat binds to Rho family GTPase 2 (Rnd2) and regulates neurite outgrowth via activation of Ras homolog gene family, member A (RhoA). [provided by RefSeq, Mar 2014] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226391 | SGK223 (Myc-DDK-tagged)-Human homolog of rat pragma of Rnd2 (SGK223) |
CNY 9592.00 |
|
| RC226391L1 | Lenti ORF clone of Human homolog of rat pragma of Rnd2 (SGK223), Myc-DDK-tagged |
CNY 11992.00 |
|
| RC226391L2 | Lenti ORF clone of Human homolog of rat pragma of Rnd2 (SGK223), mGFP tagged |
CNY 15870.00 |
|
| RC226391L3 | Lenti ORF clone of Human homolog of rat pragma of Rnd2 (SGK223), Myc-DDK-tagged |
CNY 11992.00 |
|
| RC226391L4 | Lenti ORF clone of Human homolog of rat pragma of Rnd2 (SGK223), mGFP tagged |
CNY 15870.00 |
|
| RG226391 | SGK223 (tGFP-tagged) - Human homolog of rat pragma of Rnd2 (SGK223) |
CNY 15390.00 |
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