RSPH10B2 (NM_001099697) Human Untagged Clone
CAT＃: SC316842
RSPH10B2 (untagged)-Human radial spoke head 10 homolog B2 (Chlamydomonas) (RSPH10B2)
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC316842 representing NM_001099697.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTGAAAGAAAAGAAAAAAGCAGACAAAAAAGGGGAGAAGTCTGCCCGCTCTCCCTCATCTCTCTCT GATAATCTAGACTTTTCCAAACAAGATGGCAACACCACTAGGCAAGAGATGTCCCCAGCTGGTGTCCCA TTGCTGGGAATGCAGCTCAACGAAGTGAAACCCAAAAAAGACCGCCAAAACGTTCAGCAGAACGAAGAT GCCAGCCAATACGAAGAGTCCATTCTGACCAAACTCATAGTGGAAAGCTATGAAGGGGAAAAGGTTCGT GGGCTGTATGAGGGAGAAGGCTTCGCAGCCTTTCAAGGCGGTTGTACCTATCGTGGTATGTTTTCAGAA GGACTCATGCATGGACAGGGGACTTATATTTGGGCCGATGGATTAAAATATGAGGGCGACTTTGTGAAG AATGTCCCGATGAACCACGGCGTGTACACGTGGCCGGACGGCAGCATGTATGAAGGCGAAGTGGTCAAC GGCATGAGGAACGGATTCGGGATGTTCAAGTGCAGCACCCAGCCTGTGTCCTACATCGGCCACTGGTGC AATGGCAAGCGGCACGGGAAGGGCTCCATTTATTACAATCAAGAGGGTACGTGTTGGTACGAGGGAGAC TGGGTACAAAACATCAAAAAGGGCTGGGGAATAAGATGTTATAAATCTGGAAATATATACGAAGGCCAG TGGGAAGACAACATGCGCCACGGGGAGGGGAGGATGAGGTGGCTGACCACCAACGAAGAGTACACCGGG CGGTGGGAGAGGGGCATCCAGAATGGCTTTGGAACACACACATGGTTTCTAAAGAGAATCCGCAGTTCC CAGTATCCTTTGAGAAATGAATACATAGGGGAGTTTGTAAATGGATATCGTCACGGACGTGGCAAGTTT TATTATGCCAGTGGAGCCATGTATGATGGAGAATGGGTTTCCAATAAGAAACATGGCATGGGCCGATTA ACTTTCAAGAACGGGCGTGTGTACGAAGGCGCATTCTCCAATGACCACATAGCTGGGTTTCCGGATCTT GAAGTTGAATTCATCAGCTGCCTGGACCTGTCTTCAGGAGTTGCCCCAAGACTGTCCAGGAGCGCCGAA CTGATCAGAAAGCTTGATGGCAGTGAAAGTCATTCTGTGTTGGGATCGAGCATTGAGCTGGATCTAAAT TTGCTCCTGGACATGTACCCTGAGACAGTCCAACCTGAAGAAAAGAAGCAGGTGGAATATGCCGTCTTA AGAAATATTACAGAATTAAGAAGAATTTACAGCTTTTACAGCAGCCTGGGATGCGGCCACTCTCTGGAT AATACCTTTCTGATGACAAAGCTTCACTTCTGGAGATTTCTAAAAGATTGCAAATTTCATCACCACAAA CTAACTCTTGCTGATATGGACAGGATATTAAGTGCCAATAATGACATACCAGTTGAAGAAATCCATTCT CCATTTACAACAATACTTTTGAGAACATTTTTGAATTACCTCCTGCATTTGGCGTACCACATTTATCAT GAAGAATTCCAAAAGAGAAGCCCATCCCTCTTCTTGTGTTTTACAAAACTGATGACCGAGAACATTCGT CCAAATGCCTGCCAGATAAAAGGCAATTTATTCCGTGAGCAACAGCGGACGCTCTACTCTATGAGTTAC ATGAATAAGTGCTGGGAGATTTATCTCGCTTACTGCAGACCCAGTGCAGCGCCTCCCCACGAGCCTACG ATGAAGATGAGACACTTCCTCTGGATGCTGAAAGACTTTAAAATGATAAATAAAGAATTAACAGCAGCT ACATTTATGGAGGTCATAGCAGAGGATAATCGTTTCATATATGATGGAATTGACAGCAACTTTGAACCT GAGCTGGTTTTCCTGGAATTCTTTGAAGCTCTCTTAAGCTTTGCATTCATCTGTGTTACTGACCAAATG ACTAAATCCTATACAAATGTTCCAGCTGATGATGTGTCTGGAAATAAACATGAAACTATTTATACAATA CTAAATCAGGACGCCCAGAACAAGAGTCCCAGCGCGGTCATGAGCCACGAATCGGATGCTGCTCACTCT GACAGTGCCAGGTCATCTTCCAGCAAGTTAGAACTCTCGCCTGATGTTAACAAAATAAGGAAATCAGAG CCCAAGATCAAGAAGTCTGTAAGTCATGAAAGAGTCTCCAAAATGAATTTTAAATTGACTGGAAAAGGG ATCACCTTTTTCTCATCTGAGAGCAAGAAATATGAGAGACCCAAGGATGATCGAGAGGAAGAGTTCAAC ACGTGGGTCAATAATACGTACGTCTTCTTTGTGAACACGCTCTTTCATGCGTATAAACGTGAAGAAGCT ATCAAGGAGAAAATAAGGGCAGACAGGTTACGTAGCACAGCACAGGCCCAGCAGCGGAAGATGGAAGAT GACGAACTGGAAGCAAGGCTGAACATCTTTATCTTGAGAGAGGAAGAGGCCAAGAGACATGACTATGAG GTGGACATCACAGTGCTCAAGGAGCCGGCAGACGTGTCATCCTCTCACCTCATACTGGACCCTCCCAAG GAGGATGTGACCGTGTCCCCATCCAGCAAGACCATCACCAGCAAGAAGAAGAAAAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001099697 |
| Insert Size | 2613 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001099697.1 |
| RefSeq Size | 3106 bp |
| RefSeq ORF | 2613 bp |
| Locus ID | 728194 |
| UniProt ID | B2RC85 |
| Protein Families | Druggable Genome |
| MW | 100.5 kDa |
| Gene Summary | This gene encodes a protein component of the radial spoke head in flagella and motile cilia. Eukaryotic flagella and motile cilia share a common 9 + 2 structure, in which nine peripheral microtubule doublets (MTDs) surround a central-pair of microtubules (CP), with radial spokes connecting the MTDs to the CP. The radial spoke is a multi-protein complex that works as a mechanochemical transducer between the CP and the MTDs. The radial spoke contributes to the regulation of the activity of dynein motors, and thus to flagellar motility. PMID: 22754630 provides a good review of radial spokes. [provided by RefSeq, Jul 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221344 | RSPH10B2 (Myc-DDK-tagged)-Human radial spoke head 10 homolog B2 (Chlamydomonas) (RSPH10B2) |
CNY 6376.00 |
|
| RC221344L3 | Lenti-ORF clone of RSPH10B2 (Myc-DDK-tagged)-Human radial spoke head 10 homolog B2 (Chlamydomonas) (RSPH10B2) |
CNY 9030.00 |
|
| RC221344L4 | Lenti-ORF clone of RSPH10B2 (mGFP-tagged)-Human radial spoke head 10 homolog B2 (Chlamydomonas) (RSPH10B2) |
CNY 9030.00 |
|
| RG221344 | RSPH10B2 (tGFP-tagged) - Human radial spoke head 10 homolog B2 (Chlamydomonas) (RSPH10B2) |
CNY 7976.00 |
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