DDX60L (NM_001012967) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC316608 representing NM_001012967. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGTCAAAGGATCATGCAGTATTTTTCAGGGAAATGACACAGTTAATTTTGAATGAAATGCCAAAA GCTGGGTATTCCAGCATATTAAATGATTTTGTGGAATCTAATTTTTTTGTGATTGATGGAGATTCCTTG CTTGTCACATGCCTGGGTGTAAAATCATTCAAGTGGGGACAGAATCTCCACTTTTTCTATCTGGTTGAA TGCTATCTTGTGGATCTTCTGAGTAACGGAGGACAATTCACCATAGTTTTCTTTAAGGATGCTGAATAT GCATATTTTGATTTTCCTGAACTTCTTTCATTGAGGACCGCTTTAATTCTCCACCTTCAACACAATACT AACATTGATGTGCAAACGGAGTTTTCTGGATGCTTATCACAAGATTGGAAGTTATTCTTGGAACAGCAT TACCCGTATTTTCTGATAGTTTCAGAGGAAGGCCTGAGTGATTTACAAACGTACCTTTTTAACTTCCTA ATCATACATTCCTGGGGAATGAAAGTCAATGTTGTGCTTTCATCAGGGCATGAATCTGATACTCTCAGA TTTTATGCATATACTATGGAAAGCACAGACAGAAACCAAACTTTTTCCAAGGAGAATGAAACAGTGATT CAGAGTGCATATAAAAGCCTCATACAACACTTGGAAGAAATAAGGGTTTTAGTATTAGCAACTCATTTT GAACATTTGAAATGGAATGATATGATGGAAGAGGCGTATCAGACTCTATTTCTGCTTCAGCACCTATGG TCAGAAGGATCGGACATCCAGCGTGTTCTCTGTGTCACTTCATGTTCACTATCCTTGAGAATGTACCAT CGTGTCTTAGTGCACAGTAATTGCCTATCCCTGCAGGAGGTGGAAGATTTCTGCAGACTGCGTTGCCTC TGTGTGGCTTTTCAACTCCACTTACCCCTTTCTCAGAGAGCTTGTTCTCGAGTCATCACATGCTCTTGG ATTAGGAACAGTGATTCTTTCTTAAAAATGAACAAGTGGTGTGAATATTTCATTTTAAGCAACTTAAAC GTTTTTGGATGCTGGAATCTGAATTTAAATCATGTTTCTGACTTGTATGATGAGCAATTGTTAAAGAAT ATAGCCTTCTACTATGAATTTGAAAGTACTCAAGAACCACATTTGAATTTGGGAGATTCCATTAGGAGG GATTATGAAGACCTGTGGAATGTTGTGTCACACCTGGTTAAAGAATTTAACGTTGGAAAGTCTTTTCCT CTGAGAACAACAAGAAGACATTTTCTTAGACAAGAGAAATCGGTCATTCAAGAAATCTCCTTGGAAAAG ATGCCTAGTGTGGGCTTTATTCCAATGACATCTGCTGTAATTGATGAGTTTGTTGGAGATATGATGAAG GATTTGCCTATTCTAAAGAGTGATGATCCGGTTGTTCCTTCACTGTTTAAACAAAAGACATCTGATGAA CTTTTGCACTGGCATGCTCAAAGACTCCTTAGTGACGACTATGACAGGATCAAATGTCATGTTGATGAA CAATCTAGAGATCCTCATGTTCTTGATTTCCTGAAAAAGATTCAGGATTATCAGCAATTTTATGGGAAA TCGTTAGAATCAATCTCTACGAAAGTCATTGTGACTCAAACTACTCGGCCAAAGGAGGATTCCAGTGGT GCCAGTGGTGAAATATTACAGAATACCAAACCCCACCAAATTACCAAAAAGAGTAAGAAAAAGTCATTT CTCAAAGAAGATCAGAACAAAGCTCAGCAAAACGATGATCTGCTGTTTTCTATTGAAGAGGAGATGAAG AACAATTTACATTCTGGAATAAGGAAATTGGAAGATTATTTGACATCATGTGCAAGTAATTCAGTGAAA TTTGGAGTTGAAATGTTAGGATTAATTGCCTGCTTTAAAGCATGGAAAAAACATTGCCGAGGTGAAGGC AAAATTTCGAAAGATTTAAGTATAGCTGTTCAAATGATGAAAAGGATTCATTCACTCCTGGAGAGATAC CCAGAAATTTTGGAAGCAGAACATCATCAATATATAGCTAAATGCCTTAAATATTTAGGCTTTAATGAT CTGGCAAACTCTTTGGATCCAACTCTGATAGGAGATGACAAAAATAAGAAGAAATATTCGATTGACATT GGACCAGCTCGGTTTCAACTGCAATACATGGGCCATTACTTGATAAGAGATGAAAGAAAAGATCGGGAT CCCAGGGTCCAGGATTTTATTCCCAACGCATGGCAGCAGGAACTCCTGGATGTGGTAGATAAGAATGAG TCAGCAGTGATTGTTGCCCCAACGTCCTCAGGCAAAACCTATGCTTCCTACTACTGCATGGAGAAAGTG CTGAGGGAGAGCGATGTCGGGGTGGTTGTGTACGTTGCACCCGCAAAGTCCCTTGTTGGTCAAGTGGCT GCAACTGTTGAGAATCGTTTTACTAAAACGTTGCCTGCCGGCAGAACTCTATGCGGTGCTTTTACAAGA GATTATTGTCACAATGTACTAAACTGTCAGGTACTTATTACAGTGCCGGAATGTTTTGAAATCCTGTTG CTTGCTCCTCATCGCCAAAAATGGGTGGAAAGGATCAGATATGTTATATTTGATGAGGTCCATTATCTT GGCAGAGAAGTTGGAGCAAAATTTTGGGAGCTCCTCCTTGTCATTATTCGATGTCCCTTTTTGGTTCTT TCAGCTACCATAAATAACCCAAATCTTCTCACCAAGTGGCTGCAATCAGTAAAACAGTACTGGAAACAG GCAGACAAGATTATGGAAGAGAAATGTATTTCTGAAAAACAGGCTGACAAATGTCTCAACTTTCTCCAA GACCATTCATATAAAAATCAATCATATGAAGTTAGACTTGTGCTCTGTGGAGAGAGATACAATGATTTA GAGAAGCATATATGTTCAGTAAAACATGATGATGTTTATTTTGATCATTTTCATCCCTGTGCTGCGCTA ACGACAGATATTATTGAAAAGTATGGATTCCCACCTGATCTTACCCTCACCCCTCAAGAAAGCATCCAG CTTTATGATACCATGGCTCAAGTCTGGGAAACTTGGCCCAGGGCTCAGGAATTGTGTCCAGAGGAATTC ATTCTTTTTAAGAATAAGATAGTCATTAAGAAGTTGGATGCTAGAAAATATGAAGAAAACTTAAAGGCA GAATTGACAAATTGGATTAAAAATGGCCAAGTGAAGAAGGTCAAAAGAGTACTGAAGAACCTTAGTCCG GATTCATTGTCTAGTTCAAAAGATATGGTGAAAATGTTTCCTCTTCTTGTTGAAAAGTTAAGACAAATG GATAAGTTGCCTGCAATATTTTTTTTGTTTAAGAATGATGATGTGGGAAAAAGAGCTGGAAGTGTGTGC ACTTTTCTGGAGAAGACAGAGACAAAAAGCCATCCCCACACTGAATGTCATAGTTATGTCTTTGCAATA GATGAAGTACTTGAAAAAGTGAGGAAGACACAGAAAAGGATCACTAAAAAAAACCCAAAGAAGGCTGAA AAACTGGAAAGAAAAAAAGTGTATAGAGCTGAATATATTAATTTCCTGGAGAATCTGAAGATTCTGGAA ATTTCTGAGGACTGCACGTATGCTGATGTCAAAGCCCTACACACTGAAATTACCAGGAATAAAGACTCA ACTTTGGAGAGGGTATTACCGCGAGTGCGATTTACAAGACACGGCAAAGAACTGAAGGCTTTAGCACAA AGGGGGATTGGATATCATCACAGCAGCATGTATTTTAAAGAAAAAGAGTTTGTTGAGATACTCTTTGTA AAAGGGCTTATTAGGGTAGTGACAGCTACTGAAACACTTGCCTTAGGGATCCACATGCCATGCAAATCT GTTGTTTTTGCCCAAGACTCAGTCTATCTGGATGCTTTAAATTACAGACAGATGTCTGGTCGTGCTGGA AGAAGAGGTCAAGACCTGCTTGGAAATGTGTATTTCTTTGATATCCCATTGCCCAAAATAAAAAGACTC CTTGCATCCAGTGTTCCTGAGCTGAGAGGACAGTTCCCTCTCAGCATAACCCTGGTCCTGCGACTCATG CTGCTGGCTTCCAAGGGAGATGACCCAGAGGATGCCAAGGCAAAGGTGTTGTCAGTGCTAAAGCATTCA TTGCTGTCTTTTAAGAGACGAAGAGCCATGGAGACTTTGAAACTTTACTTTTTGTTTTCCTTGCAGCTC CTTATCAAAGAGGACTATTTAAATAAAAAGGGTAATCCAAAGAAATTTGCAGGACTTGCATCATATTTG CATGGTCATGAACCTTCAAATCTTGTTTTTGTAAATTTTCTCAAGAGAGGCCTTTTCCATAATCTCTGT AAGCCAGCCTGGAAAGGCTCACAACAATTTTCCCAAGATGTGATGGAAAAGCTCGTGTTAGTATTGGCA AATTTGTTTGGAAGAAAATATATTCCAGCAAAATTCCAAAATGCTAATTTAAGTTTTTCTCAGTCAAAG GTGATCCTTGCCGAACTCCCGGAGGATTTTAAAGCTGCTTTATATGAGTATAACCTGGCAGTAATGAAG GATTTTGCCTCCTTCCTGCTGATTGCTTCCAAGTCGGTGAACATGAAAAAAGAGCATCAACTCCCTTTG TCAAGAATCAAATTCACAGGTAAAGAATGTGAAGACTCCCAACTCGTGTCTCACTTGATGAGCTGCAAG AAAGGAAGAGTAGCCATTTCACCATTTGTTTGTCTTTCGGGGAACACAGATAATGATTTGCTTCGACCA GAGACTATCAACCAGGTCATCCTGCGCACAGTCGGTGTTAGTGGCACTCAGGCTCCTCTGCTGTGGCCA TGGAAATTAGATAACCGAGGAAGGAGAATGCCACTAAATGCATATGTGCTCAATTTCTATAAACACAAC TGCTTGACAAGATTAGACCAAAAAAATGGGATGCGTATGGGACAGCTTTTAAAGTGTTTGAAAGATTTT GCATTCAACATTCAGGCTATCAGTGACTCCTTGAGTGAACTATGTGAAAATAAGCGTGACAATGTAGTC CTGGCATTTAAACAATTGAGTCAAACCTTTTATGAGAAACTTCAAGAAATGCAAATTCAAATGAGTCAA AATCATTTAGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001012967
Insert Size	5121 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001012967.2
RefSeq Size	6781 bp
RefSeq ORF	5121 bp
Locus ID	91351
UniProt ID	Q5H9U9
MW	197.6 kDa
Gene Summary	This gene encodes a member of the DExD/H-box helicase family of proteins, a subset of the super family 2 helicases. Members of the DExD/H-box helicase family share a conserved functional core comprised of two RecA-like globular domains. These domains contain conserved motifs that mediate ATP binding, ATP hydrolysis, nucleic acid binding, and RNA unwinding. In addition to functions in RNA metabolism, members of this family are involved in anti-viral immunity and act as cytosolic sensors of viral nucleic acids. The protein encoded by this gene has been shown to inhibit hepatitis C virus replication in response to interferon stimulation in cell culture. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]

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