CEP78 (NM_032171) Human Untagged Clone
CAT＃: SC316565
CEP78 (untagged)-Human centrosomal protein 78kDa (CEP78), transcript variant 2
CNY 13590.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C9orf81; CRDHL; IP63 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC316565 representing NM_032171.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATCGACTCCGTGAAGCTGCGCCGCGACAGCGCGGCGGACTTCTTCTCCCACTACGAGTACCTGTGC GCGCTGCAGAACTCGGTGCCGCTGCCCGCCGTGCGCGCCTGTCTCCGGGAGGGCGTGCTGGATTTCAAC GCCGACCGCCTCCGCGGGGTGGACTGGGCGCCTCTGCTGAGCACCCTCAAGATCAATAAAGACCTGCCC TTGGTCTCCATCAAGAGCTTCTTCCAGCCCTGGCTGGGGGACACAGGTTCTGACATGAATAAATTTTGC AGAAGTCGTGTTCCTGCGATAAGATACAAAGATGTGACCTTCCAGTTGTGTAAAGCTCTTAAAGGCTGT TTAAGTATATCAAGTGTGCTAAAGAACCTGGAGCTAAATGGACTAATTCTGAGAGAGAGGGATTTAACT ATTCTAGCAAAGGGATTGAATAAATCGGCTTCTTTGGTGCACCTGTCTCTTGCAAATTGTCCAATTGGA GATGGAGGTTTAGAAATTATTTGTCAAGGTATAAAGAGCTCTATCACTCTTAAGACAGTCAACTTCACA GGATGTAATCTGACATGGCAGGGAGCAGATCACATGGCCAAGATCTTAAAGTATCAGACCATGAGAAGG CATGAAGAAACCTGGGCTGAGAGTCTTCGCTATAGGAGACCTGATCTTGACTGTATGGCTGGCTTAAGA CGTATCACACTGAATTGCAACACACTTATTGGTGACCTAGGTGCATGTGCTTTTGCAGACTCTCTCAGT GAGGATTTATGGCTGAGAGCTCTTGACCTGCAACAGTGCGGCCTCACCAATGAAGGAGCAAAGGCTTTG CTAGAGGCCCTTGAAACCAATACAACTCTGGTCGTTCTGGATATAAGAAAAAATCCACTCATTGATCAT TCTATGATGAAAGCAGTTATCAAAAAAGTCCTCCAGAATGGAAGGAGTGCCAAATCAGAGTACCAGTGG ATAACTTCTCCATCAGTGAAGGAACCATCCAAAACTGCTAAACAGAAAAGGAGAACTATAATTCTAGGA AGTGGTCACAAAGGAAAAGCTACTATTAGAATTGTAGGATTGGCTACAAAGAAACCTGTAAGTAGTGGC AGAAAACACTCCCTTGGTAAAGAATATTATGCGCCCGCACCTCTTCCACCTGGTGTGTCTGGTTTCTTG CCGTGGCGTACTGCAGAACGTGCAAAAAGACACAGGGGTTTCCCATTAATCAAAACACGTGATATATGT AATCAGTTGCAGCAACCAGGTTTTCCTGTGACTGTGACAGTAGAGAGTCCTTCATCCTCTGAAGTTGAA GAGGTTGATGATTCTTCAGAGAGTGTTCATGAAGTGCCTGAGAAAACTAGTATAGAACAAGAAGCATTA CAGGAAAAACTGGAGGAGTGCCTAAAGCAGTTAAAGGAAGAAAGAGTGATAAGGCTTAAGGTTGATAAA CGAGTCAGTGAGCTGGAACATGAAAATGCCCAGTTAAGAAATATAAATTTCTCTTTGTCTGAAGCCCTT CATGCACAGTCATTGACAAATATGATCCTGGATGATGAAGGTGTTTTGGGCAGCATTGAGAATTCTTTT CAGAAGTTTCATGCTTTCTTGGATCTCCTTAAAGATGCTGGGCTTGGGCAGCTTGCCACAATGGCTGGG ATAGATCAGTCAGATTTTCAATTACTAGGTCATCCCCAGATGACTTCTACTGTTAGTAATCCACCTAAA GAAGAAAAGAAGGCGCTTGAAGATGAAAAACCAGAACCGAAGCAGAATGCCCTAGGGCAAATGCAAAAT ATCCAGTTTCAGAAAATTACAGGTGATGCTAGAATTCCTTTGCCTCTCGACTCCTTTCCTGTCCCAGTT TCTACTCCAGAGGGCTTAGGAACTTCCAGCAACAACCTAGGAGTCCCAGCTACTGAGCAGCGGCAGGAG TCTTTTGAAGGATTCATTGCTAGAATGTGTTCTCCTTCACCAGATGCGACTTCTGGAACTGGAAGTCAA AGAAAAGAAGAGGAGTTGTCCAGAAATAGCAGATCTTCTTCAGAGAAAAAGACCAAAACAGGTGAATAT ACCAAAAAACACTCTGATAAGCAACACCCTGGAAAGGACCTGCATTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_032171 |
| Insert Size | 2121 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032171.1 |
| RefSeq Size | 2719 bp |
| RefSeq ORF | 2121 bp |
| Locus ID | 84131 |
| UniProt ID | Q5JTW2 |
| MW | 78.3 kDa |
| Gene Summary | This gene encodes a centrosomal protein that is both required for the regulation of centrosome-related events during the cell cycle, and required for ciliogenesis. The encoded protein has an N-terminal leucine-rich repeat (LRR) domain with six consecutive LRR repeats, and a C-terminal coiled-coil domain. It interacts with the N-terminal catalytic domain of polo-like kinase 4 (PLK4) and colocalizes with PLK4 to the distal end of the centriole. Naturally occurring mutations in this gene cause defects in primary cilia that result in retinal degeneration and sensorineural hearing loss which are associated with cone-rod degeneration disease as well as Usher syndrome. Low expression of this gene is associated with poor prognosis of colorectal cancer patients. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region compared to variant 1. The encoded isoform (b) is shorter than isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220007 | CEP78 (Myc-DDK-tagged)-Human centrosomal protein 78kDa (CEP78), transcript variant 2 |
CNY 5168.00 |
|
| RC220007L1 | Lenti ORF clone of Human centrosomal protein 78kDa (CEP78), transcript variant 2, Myc-DDK-tagged |
CNY 9310.00 |
|
| RC220007L2 | Lenti ORF clone of Human centrosomal protein 78kDa (CEP78), transcript variant 2, mGFP tagged |
CNY 9310.00 |
|
| RC220007L3 | Lenti ORF clone of Human centrosomal protein 78kDa (CEP78), transcript variant 2, Myc-DDK-tagged |
CNY 9310.00 |
|
| RC220007L4 | Lenti ORF clone of Human centrosomal protein 78kDa (CEP78), transcript variant 2, mGFP tagged |
CNY 9310.00 |
|
| RG220007 | CEP78 (tGFP-tagged) - Human centrosomal protein 78kDa (CEP78), transcript variant 2 |
CNY 8170.00 |
