Myosin IIIB (MYO3B) (NM_001083615) Human Untagged Clone
CAT＃: SC316082
MYO3B (untagged)-Human myosin IIIB (MYO3B), transcript variant 1
CNY 25370.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC316082 representing NM_001083615.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAACATCTGTATGGATTATTTCACTATAATCCTATGATGCTTGGACTTGAATCACTTCCAGATCCC ACAGACACCTGGGAAATTATAGAGACCATTGGTAAAGGCACCTATGGCAAAGTCTACAAGGTAACTAAC AAGAGAGATGGGAGCCTGGCTGCAGTGAAAATTCTGGATCCAGTCAGTGATATGGATGAAGAAATTGAG GCAGAATACAACATTTTGCAGTTCCTTCCTAATCATCCCAATGTTGTAAAGTTTTATGGGATGTTTTAC AAAGCGGATCACTGTGTAGGGGGACAGCTGTGGCTGGTCCTGGAGCTGTGTAATGGGGGCTCAGTCACT GAGCTTGTCAAAGGTCTACTCAGATGTGGCCAGCGGTTGGATGAAGCAATGATCTCATACATCTTGTAC GGGGCCCTCTTGGGCCTTCAGCATTTGCACAACAACCGAATCATCCACCGTGATGTGAAGGGGAATAAC ATTCTTCTGACAACAGAAGGAGGAGTTAAGCTCGTTGACTTTGGTGTTTCAGCTCAACTCACCAGTACA CGTCTGCGGAGAAACACATCTGTTGGCACCCCGTTCTGGATGGCCCCTGAGGTCATTGCCTGTGAGCAG CAGTATGACTCTTCCTATGACGCTCGCTGTGACGTCTGGTCCTTGGGGATCACAGCTATTGAACTGGGG GATGGAGACCCTCCCCTCTTTGACATGCATCCTGTGAAAACACTCTTTAAGATTCCAAGAAATCCTCCA CCTACTTTACTTCATCCAGAAAAATGGTGTGAAGAATTCAACCACTTTATTTCACAGTGTCTTATTAAG GATTTTGAAAGGCGACCTTCCGTCACACATCTCCTTGACCACCCATTTATTAAAGGAGTACATGGAAAA GTTCTGTTTCTGCAAAAACAGCTGGCCAAGGTTCTCCAAGACCAGAAGCATCAAAATCCTGTTGCTAAA ACCAGGCATGAGAGGATGCATACCAGAAGACCTTATCATGTGGAAGATGCTGAAAAATACTGCCTTGAG GATGATTTGGTCAACCTAGAGGTTCTGGATGAGGATACAATTATCCATCAGTTGCAGAAACGTTATGCA GACTTGCTAATTTACACATATGTTGGAGACATCTTAATTGCCTTAAACCCCTTCCAGAATCTAAGCATA TACTCTCCACAGTTTTCCAGACTTTATCATGGGGTGAAACGCGCCTCCAATCCCCCCCACATATTTGCA TCAGCAGATGCTGCTTACCAGTGCATGGTTACTCTCAGCAAAGACCAGTGCATTGTCATCAGCGGAGAG AGTGGCTCTGGGAAGACAGAAAGCGCCCACCTGATTGTTCAGCATTTGACTTTCTTGGGAAAGGCCAAT AATCAGACCTTGAGAGAGAAAATTCTACAAGTCAACTCCCTGGTGGAAGCCTTTGGGAACTCATGCACT GCCATCAATGACAACTCGAGCCGTTTTGGAAAATATCTGGAAATGATGTTTACACCAACTGGAGTTGTG ATGGGGGCAAGAATCTCTGAATATCTCCTGGAAAAATCCAGAGTTATAAAACAGGCAGCGAGAGAGAAA AATTTTCATATATTTTACTATATTTATGCTGGTCTTCATCACCAAAAGAAGCTTTCTGATTTCAGACTT CCTGAGGAAAAACCTCCTAGGTACATAGCTGATGAAACTGGAAGGGTGATGCACGACATAACTTCCAAG GAGTCTTACAGAAGACAATTCGAAGCAATTCAGCATTGCTTCAGGATTATAGGGTTCACGGACAAAGAG GTGCACTCAGTGTACAGAATTTTGGCTGGGATTTTGAATATTGGGAACATTGAGTTCGCAGCTATTTCC TCTCAACATCAGACTGATAAAAGTGAGGTGCCCAATGCTGAAGCTTTGCAAAATGCTGCCTCTGTTCTG TGCATTAGCCCTGAAGAGCTCCAGGAGGCCCTCACCTCCCACTGTGTGGTCACCCGGGGCGAGACCATC ATCCGTGCCAACACTGTAGACAGGGCTGCGGACGTTCGAGACGCCATGTCCAAAGCCCTGTATGGGAGG CTCTTCAGCTGGATTGTGAATCGCATTAATACACTCCTGCAGCCAGACGAAAACATATGTAGTGCAGGA GGTGGAATGAATGTGGGGATCTTGGATATCTTTGGATTCGAGAATTTTCAGAGAAATTCATTTGAGCAG CTCTGCATAAACATCGCCAATGAGCAAATCCAGTACTATTTCAATCAGCATGTTTTTGCTCTTGAGCAG ATGGAATATCAGAATGAAGGCATTGATGCTGTACCCGTGGAATATGAGGACAACCGCCCGCTCTTGGAC ATGTTCCTCCAGAAACCCCTGGGACTGCTTGCACTTTTGGATGAGGAAAGTCGGTTTCCCCAAGCAACT GACCAGACCCTGGTTGATAAATTTGAAGATAATCTACGATGCAAATACTTCTGGAGGCCCAAAGGAGTG GAACTGTGCTTTGGCATTCAGCATTATGCTGGAAAGGTATTATATGATGCTTCTGGGGTTCTTGAGAAA AATAGAGACACTCTCCCTGCCGATGTGGTTGTGGTCCTGAGAACGTCAGAAAACAAGCTTCTTCAGCAG CTCTTCTCAATCCCTCTGACCAAAACAGGTAATTTGGCCCAGACAAGAGCTAGGATAACAGTGGCCTCA AGTTCTTTGCCTCCACATTTCAGTGCTGGGAAAGCCAAGGTGGACACTCTGGAGGTGATACGGCATCCG GAAGAAACCACCAACATGAAGAGGCAAACTGTGGCTTCTTACTTCCGGTATTCTCTGATGGACCTGCTC TCCAAAATGGTGGTTGGACAGCCCCACTTTGTGCGCTGCATTAAACCCAATGATGACCGAGAGGCCCTG CAGTTCTCTCGAGAGAGGGTGCTGGCCCAGCTCCGCTCCACAGGGATTCTGGAGACAGTCAGCATCCGC CGCCAGGGCTATTCCCACCGCATCCTTTTTGAAGAATTTGTGAAAAGGTATTATTACTTGGCATTCACA GCACATCAAACACCTCTTGCTAGCAAAGAGAGCTGTGTGGCTATCTTGGAAAAGTCCAGATTAGATCAC TGGGTACTGGGAAAAACAAAGGTTTTTCTCAAATATTACCATGTTGAGCAATTAAATTTGCTGCTTCGA GAAGTCATAGGCAGAGTGGTTGTGCTGCAGGCATATACCAAGGGGTGGCTTGGAGCCAGGAGATACAAA AGGGTCAGAGAGAAGAGAGAGAAGGGAGCCATTGCCATCCAGTCAGGGGACACTTCAAACCAAAGCAGT GGGCCACATTCCCCCGTCGCAGCAGGTACGAGGGGAAGTGCCGAGGTTCAAGACTGCAGCGAGCCTGGT GACCATAAAGTTCTCAGGGGCTCTGTACATCGTAGGAGCCATTCACAAGCAGAATCCAACAATGGCCGT ACACAGACTTCAAGCAACTCTCCTGCTGTCACAGAGAAAAATGGGCATTCACAAGCCCAGAGTTCTCCA AAAGGGTGCGATATCTTCGCAGGACATGCAAACAAGCACTCGGTTTCTGGGACTGATTTGCTGTCTTCT CGGATATGCCATCCTGCTCCAGATCAGCAAGGATTGAGTCTCTGGGGAGCCCCTCAAAAGCCTGGTTCA GAAAATGGTCTTGCACAGAAGCATCGAACACCTCGCCGACGATGTCAGCAGCCCAAAATGCTGAGTAGC CCTGAGGACACCATGTACTATAACCAGTTAAATGGAACTCTAGAATATCAAGGGAGCAAGAGGAAGCCA AGAAAACTTGGCCAAATCAAAGTACTTGATGGGGAAGATGAATATTACAAATCTCTGTCACCAGTGGAC TGTATCCCTGAGGAGAACAACTCAGCCCACCCTTCCTTTTTTTCTTCATCCTCAAAAGGAGACTCTTTT GCTCAACATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001083615 |
| Insert Size | 3945 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001083615.3 |
| RefSeq Size | 6149 bp |
| RefSeq ORF | 3945 bp |
| Locus ID | 140469 |
| UniProt ID | Q8WXR4 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 148.6 kDa |
| Gene Summary | This gene encodes one of the class III myosins. Myosins are ATPases, activated by actin, that move along actin filaments in the cell. This class of myosins are characterized by an amino-terminal kinase domain and shown to be present in photoreceptors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (1) lacks an alternate in-frame exon in the coding region, compared to variant 2. This results in a shorter protein (isoform 1), compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216078 | MYO3B (Myc-DDK-tagged)-Human myosin IIIB (MYO3B), transcript variant 1 |
CNY 9152.00 |
|
| RC216078L3 | Lenti ORF clone of Human myosin IIIB (MYO3B), transcript variant 1, Myc-DDK-tagged |
CNY 15010.00 |
|
| RC216078L4 | Lenti ORF clone of Human myosin IIIB (MYO3B), transcript variant 1, mGFP tagged |
CNY 15010.00 |
|
| RG216078 | MYO3B (tGFP-tagged) - Human myosin IIIB (MYO3B), transcript variant 1 |
CNY 14440.00 |
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