OC90 (NM_001080399) Human Untagged Clone
CAT＃: SC315773
OC90 (untagged)-Human otoconin 90 (OC90)
CNY 7700.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PLA2L |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC315773 representing NM_001080399.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATTGCGTTTCTCCTCACCAGTGTGCTGATGATCCCCCATGCCGGAGGCCATCCTCTGGACACTCCA CATCTTCCACAGGAGCTGCCTCCAGGACTCCCAAACAATATCAATATCACTTTCTTCAGTGGGATGTTT AAAAATGTGGAAAGTGTGGCTGAAATTTTTGATTGCCTGGGCCCCCACTTCACCTGGCTGCAGGCTGTC TTCACCAATTTCCCTGTGCTGATCCAGTTTGTCAATGGTATGAAGTGTGTGGCTGGTCTCTGCCCCCGA GACTTTGAAGACTATGGTTGCACCTGCAGGTTTGAGATGGAAGGGTTGCCTGTGGATGAATCTGACAGC TGCTGCTTCCAGCACCGCAGGTGCTATGAGGAGGCCGCTGAGATGGACTGTCTCCAAGACCCCGCCAAA CTTAGCACAGAGGTCAATTGTGTCAGCAAGAAGATCATATGTGAGTCCAAGGACAACTGTGAGCACCTG CTGTGTACCTGTGATAAGGCTGCCATAGAGTGCTTGGCTCGATCCAGCCTCAACTCTTCCCTGAACCTT CTGGACACCTCCTTCTGCCTGGCTCAGACTCCAGAGACAACCATCAAGGAAGACTTGACAACACTTCTG CCCAGAGTGGTTCCTGTGGAGCCCACAGACACCAGCCTGACAGCCCTTTCAGGAGAAGAAGCAGGCCAC GATCAGGAAGGAGTGGGAGCTGCTAGGGCTACGTCCCCTCCAGGATCTGCAGAGATAGTTGCAACAAGG GTTACAGCTAAAATTGTAACCCTTGTCCCTGCTGGCATTAAATCTCTGGGGCTGGCAGTGTCATCTGTT GAAAATGATCCTGAGGAGACCACTGAAAAAGCCTGTGACAGATTCACCTTCCTGCACCTGGGAAGTGGG GACAACATGCAGGTGATGCCACAGCTTGGAGAGATGCTCTTTTGTCTGACATCCCGGTGCCCGGAGGAA TTTGAGTCTTATGGCTGTTACTGTGGACAAGAAGGAAGAGGCGAGCCAAGGGATGACCTAGACAGGTGC TGCTTGTCCCATCACTGCTGCCTAGAGCAAGTGAGAAGGCTGGGCTGCCTGCTTGAGAGGCTTCCTTGG TCACCGGTGGTGTGTGTGGATCATACGCCCAAGTGTGGGGGCCAAAGCCTGTGTGAGAAGTTGCTCTGT GCCTGTGACCAGACGGCAGCTGAGTGCATGACCTCTGCCTCCTTTAACCAAAGCCTCAAGTCCCCAAGC AGACTCGGGTGCCCTGGGCAGCCAGCAGCCTGTGAAGACAGCCTGCACCCTGTGCCCGCAGCCCCCACC CTGGGCTCCAGCTCTGAGGAGGACAGCGAGGAGGACCCTCCACAGGAGGACCTCGGCAGAGCCAAGAGG TTTCTGCGGAAGTCACTGGGTCCCTTGGGGATCGGGCCTCTTCATGGAAGATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001080399 |
| Insert Size | 1434 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001080399.2 |
| RefSeq Size | 1782 bp |
| RefSeq ORF | 1434 bp |
| Locus ID | 729330 |
| UniProt ID | Q02509 |
| Protein Families | Secreted Protein |
| MW | 51.7 kDa |
| Gene Summary | Major protein of the otoconia, a calcium carbonate structure in the saccule and utricle of the ear. Together with OTOL1, acts as a scaffold for otoconia biomineralization: sequesters calcium and forms interconnecting fibrils between otoconia that are incorporated into the calcium crystal structure. Together with OTOL1, modulates calcite crystal morphology and growth kinetics. It is unlikely that this protein has phospholipase A2 activity.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221622 | OC90 (Myc-DDK-tagged)-Human otoconin 90 (OC90) |
CNY 3656.00 |
|
| RC221622L3 | Lenti ORF clone of Human otoconin 90 (OC90), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC221622L4 | Lenti ORF clone of Human otoconin 90 (OC90), mGFP tagged |
CNY 5890.00 |
|
| RG221622 | OC90 (tGFP-tagged) - Human otoconin 90 (OC90) |
CNY 4370.00 |
